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Biomedical subjects

M Büttner

Publications and source records attributed to M Büttner.

At least 55 records · Page 3Linked to original sources

[Extraskeletal Ewing sarcoma: differential diagnosis of a soft tissue tumor].

We report on a 30-year-old man from Vietnam who complained about diffuse pain in the left epigastrium. Using the ultrasound a pleural effusion in the left recessus costodiaphragmaticus was found. The chest X-ray showed a paravertebral soft-tissue tumor at the left side, with CT and NMR a sarcoma was suspected. The patient was treated by local resection of the tumor, histologically an extraosseous Ewing's sarcoma was identified. Due to the following chemotherapy and local radiation therapy no local recurrence and metastases have developed for 32 months.

Adult↗

[Behavior of Orf virus in permissive and nonpermissive systems].

Dogs were immunized i.m. with attenuated poxvirus vaccines (vaccinia virus, Orf-virus) and a bovine herpesvirus-1 (BHV-1) vaccine. After intradermal (i.d.) application of the vaccine viruses a specific delayed type hypersensitivity (DTH) reaction of the skin occurred only with vaccinia virus. The i.d. application of Orf-virus caused a short-term, non-specific inflammatory reaction of the skin, even in dogs not immunized with Orf-virus. Out of 30 sera from Orf-virus immunized beagles (n = 4) only eight were found reactive to Orf-virus in a competition ELISA. Three sera from dogs not Orf-virus immunized but skin-tested with the virus contained low antibody titers. Using indirect immunofluorescence (IIF) in flow cytometry, the existence of Orf-virus antigens was examined on the surface and in the cytoplasm of permissive (BFK and Vero)- and questionable permissive MDCK cells. The canine kidney MDCK cell line was found to be non-permissive for Orf-virus replication; the occurrence of an Orf-(ecthyma contagiosum) like disease in dogs is unlikely.

Animals↗

[What physicians should currently observe in the area of dental health].

Several factors govern the development of human dental caries. The actual caries incidence in children depends greatly on fluoride consumption, reduction of fermentable sugar intake (in favour of the use of sugar substitutes), dental health habits and regular dental check-ups. A significant reduction of dental caries in children and young adults is achieved in many countries following these principles. However, there remains a group, about 25%, with a particularly high incidence of caries. Today, efforts are directed at detecting these risk subjects. Mutans streptococci and lactobacilli are the main causative bacteria of human dental caries. The initiation of dental caries is preceded by colonisation of the dentition by S. mutans, usually in early childhood. The early establishment of these bacteria in the mouth of human infants is dependent on intrafamilial--mainly maternal--transmission of saliva. The higher the level of S. mutans in the maternal saliva, the more likely is the colonisation of primary dentition by cariogenic bacteria. The earlier the colonisation, the higher the incidence of caries. Today we have new diagnostic aids: Simple microbiological tests to detect the individuals with high salivary levels of these cariogenic bacteria, so called SM-millionaires. The tests are also a good educational aid for motivating patients to individual prophylaxis. What could a paediatrician or gynaecologist do to help the dentist? He could inform the parents early enough to avoid saliva contacts. For example, the mother should not take the child's spoon or pacifier in her mouth. The dentist meets the children much later, at an age where they already have caries. The older methods, mentioned at the begin, are also still valuable.

Adolescent↗

[Clinical findings and diagnosis of a severs parapoxvirus epidemic in Finnish reindeer].

During the winter of 1992/93 a parapox epidemic in reindeer occurred in the southern and central areas of Finland which killed 400 animals and 2750 animals showed clinical symptoms. At least ten cases of human infections and disease were reported. In addition to the uncertain epidemiology, the diagnosis using conventional methods was difficult and time consuming. Based upon published sequence data of the parapoxvirus (PPV) ovis prototype strain NZ-2, two different polymerase chain reaction (PCR) protocols were performed. The detection of a PPV specific nucleotide sequence encoding a region of the 42 kD major envelope protein proved to be reproducible. DNA extraction from scab samples was not absolutely necessary prior to the PCR procedure. PCR for the detection of PPV infection can be recommended as a flanking diagnostic method, especially when electron microscopy shows negative results. PCR may be a useful method to differentiate sheep-poxvirus and to reveal epidemiology and identity of new PPV isolates.

Animals↗

Colonic-crypt-derived epithelia express induced ion transport differentiation in monolayer cultures on permeable matrix substrata.

The processes of transport differentiation from stem cell to the terminally differentiated cell in intact colonic crypts are difficult to study because access to the lumen is limited. Colonocytes were isolated from the lower two-thirds of rat distal colon crypts and grown to confluence on reconstituted basement membranes and permeable support in primary culture. Crypt and surface cells were distinguished by the uptake of [3H]thymidine and [3H]leucine and by brushborder fluorescence binding. Ion concentrations in apical and basolateral compartments of filter monolayer cultures after 48 h of incubation on days 16-18 were (in mM): apical, Na+ 116 +/- 4 (n = 48) and K+ 6 +/- 1 (n = 48); basolateral, Na+ 151 +/- 3 and K+ 3.7 +/- 0.5, respectively (mean +/- SE). Aldosterone (10(-8) M), added to the basolateral compartment from days 10-18, changed apical Na+ to 72 +/- 6 mM and apical K+ to 13 +/- 4 mM (n = 23). Dexamethasone (10(-8) M) changed apical Na+ to 84 +/- 7 mM but did not influence apical K+ (n = 22). Transmonolayer electrical potential difference (VtM; control medium; days 8-10) was 5 +/- 1 mV (n = 16; apical compartment negative); electrical resistance (RtM) was 217 +/- 21 omega.cm2 and short circuit current (ISC) was 21 +/- 5 microA.cm-2. Amiloride (0.1 mM; n = 12) in the apical medium decreased VtM to 2 +/- 1 mV and ISC to 11 +/- 4 microA.cm-2. Aldosterone (10(-8) M) after 1 week in the basolateral compartment (n = 21) changed VtM to 12.3 +/- 3 mV, RtM to 92 +/- 9 omega.cm2, and ISC to 138 +/- 23 microA.cm-2. Apical amiloride (0.1 mM; n = 9) decreased the induced VtM to -3 +/- 1 mV and ISC to -13 +/- 7 microA.cm-2. Colonic-crypt-derived epithelial cells proliferate and differentiate in primary culture, when grown on reconstituted basement membrane substratum and in supplemented medium, to form monolayers that express net Na+ absorption and net K+ secretion after 1 week. Na+ and K+ vectorial transport differentiation is primarily regulated by aldosterone, which specifically induces apical conductive Na+ transfer. Mineralocorticoid and glucocorticoid hormones appear to have differing actions on ion transport in functionally surface-type colonocytes derived in culture from isolated crypt-type cells.

Aldosterone↗

More than one component of the Newcastle disease virus particle is capable of interferon induction.

The interferon (IFN)-inducing capacities of intact NDV virions, beta-propiolactone-inactivated particles and several structural components were compared, using human PBML as the IFN producing cells. Intact and inactivated virions as well as the nucleocapsid fraction did not differ significantly in their IFN-inducing capacity. In contrast, genomic RNA as well as M protein fraction and envelopes induced IFN titres to a level of about 10% of those achieved with virions. NDV-induced IFN production could be blocked specifically by incubation with polyclonal anti-NDV-monoclonal antibodies (mAbs) and with two of three anti-HN-mAbs, but not with anti-NDV-mAbs directed against the F, M or NP protein. In addition, IFN induction by fixed MDBK cells, expressing NDV surface proteins after infection with NDV Ulster, was inhibited by one of two anti-F-mAbs. The results suggest that the induction of IFN synthesis in human PBML is a complex process involving not only the HN protein but also the uncleaved F protein precursor, a component of the M protein fraction and--once having entered the cell--the genomic RNA.

Animals↗

A conserved loop in the ATPase domain of the DnaK chaperone is essential for stable binding of GrpE.

The activity of DnaK (Hsp70) chaperones in assisting protein folding relies on DnaK binding and ATP-controlled release of protein substrates. The ATPase activity of DnaK is tightly controlled by the nucleotide exchange factor GrpE. We find that GrpE interacts stably with the amino-terminal ATPase domain of DnaK. Analysis of the mutant DnaK756 protein, which has a lower affinity for GrpE, reveals a role for residue Gly 32 in GrpE binding. Gly 32 is located in an exposed loop near the nucleotide binding site of DnaK. Deletion of this loop prevents stable GrpE binding, ATPase stimulation by GrpE, and DnaK chaperone activity. Conservation of this loop within the Hsp70 family suggests that cooperation between Hsp70 and GrpE-like proteins may be a general feature of this class of chaperone.

Adenosine Triphosphatases↗

Prevention of graft-versus-host disease in DLA-haplotype mismatched dogs and hemopoietic engraftment of CD6-depleted marrow with and without cG-CSF treatment after transplantation.

Prevention of graft-versus-host disease by depletion of CD6-positive T cells was studied in the dog. Donors were DLA-homozygous, recipients DLA-heterozygous with one DLA haplotype identical to the donor. Seven control dogs received untreated marrow and died of GvHD after full hemopoietic recovery within 28 days of transplantation. For prevention of GvHD, immunomagnetic separation of T cells with a monoclonal antibody against human CD6 that crossreacted with canine T cells was evaluated. Depletion of CD6-positive cells depleted CD4-positive cells completely, but only part of CD8-positive cells and DR-positive cells. CD6-depleted marrow exhibited strong nonspecific "natural" suppression of the generation of cytotoxic T cells in vitro. Eleven dogs received CD6-depleted marrow. Only 1 dog developed GvHD and died. Sustained engraftment was seen in 8 dogs. Hemopoietic recovery was delayed and slower after transplantation of CD6-depleted marrow than after transplantation of untreated marrow. Four of these dogs were treated with G-CSF, and this accelerated the recovery of leukocytes, but did not prevent rejection. Chimerism was mixed in 7 of 10 evaluable dogs and 1 dog recovered its own hemopoiesis 2 years after transplantation. CD6 depletion prevents GvHD across a DLA-haplotype difference, but rejection and mixed chimerism may occur. Treatment with G-CSF accelerates leukocyte recovery, but cannot prevent rejection.

Animals↗

[Recent developments in the diagnosis of parapoxviruses].

Neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (PPV) were generated by immunizing BALB/c-mice with gradient-purified PPV Orf D-1701 or purified envelopes. Epitope specificity studies identified three distinct epitopes localized in the virus envelope. These antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. For a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, antigen capture ELISA, and polymerase-chain-reaction were used.

Animals↗

[Salivary concentration of Streptococcus mutans in children and adolescents in Basel].

The search for a feasible method to estimate the caries risk in humans is going on since several years. Such risk assessments should especially be performed in persons clinically suspected to be at risk. The quantitative determination of Streptococcus mutants (SM) in whole saliva has been proposed to serve as a measure of the caries risk. SM tests have been performed in 1991 in a group of 5743 5 to 16 year old children and adolescents living in the city of Basle. Levels of SM in whole saliva were correlated with caries prevalence both in the deciduous and permanent teeth. SM tests have equally become a helpful pedagogic instrument used to motivate patients for individual prophylactic activities.

Adolescent↗

[Is dental caries contagious?].

Results from microbiological studies and saliva analyses have now become valuable diagnostic aids which we could no longer imagine being without. They give us additional, objective information on the clinical and radiological examination of caries. These tests have also proved to be valuable pedagogical aids for motivating patients in individual prophylaxis. The question asked at the beginning, "Are caries infectious?" can be answered as follows: Yes, the possibility of transmitting caries exists mainly during the first three years of an infant's life and when the molars erupt in the sixth and twelfth years. Transmission is avoidable when one knows the various ways in which it is transmitted. Information on this and knowledge among the general public is still, however, in its infancy.

Child↗

[Clinical aspects and diagnosis of border disease].

The term Border disease (BD) refers to a clinical condition of newborn lambs that results from congenital infection by a pestivirus (family: flaviviridae), occurring during the first one-half of gestation. The most prominent clinical features are tonic-clonic tremors and hairy fleece ("hairy shaker" symptomatology). Moreover early transplacental infection often causes fetal death, abortion or stillbirth and teratogenic disorders. In Germany there are only few reports on clinical cases of BD, however in sheep antibodies have been found frequently in different regions of the country. Similar to the situation with bovine viral diarrhea/mucosal disease (BVD/MD) the existence of persistently infected sheep is proven. As permanent carriers and initiators of clinically apparent as well as more commonly clinically inapparent infections persistently infected animals are of great epidemiological importance. As a new method for the diagnosis of persistently Border disease Virus (BDV) infected sheep flow cytometry is presented. This special procedure of immunofluorescence has the overall advantage of rapid and reliable detection of pestivirus-infected cells by a highly conserved viral epitope (p 80/125). All biotypes of pestiviruses (non cytopathic = ncp and cytopathic = cp isolates) are recognized equally well by monoclonal antibodies (mAb) which provides some advantage compared to conventional techniques because most virus isolates from sheep belong to the ncp biotype.

Animals↗

Principles of paramunization. Option and limits in veterinary medicine.

The so-called primitive immune system has not changed during evolution. Even in primates it plays the most important role in first line defence against invading microorganisms. Cellular components such as macrophages, granulocytes, Natural Killer cells and gamma delta-T cells and soluble humoral factors--the cytokines--are the representatives of the primitive immune system. An interlocking communicative network regulates flexible response of effector cells towards "non-self" antigens. It also ensures close connection with the repertoire of specific immune response, e.g. antibody formation. Multifactorial diseases, nosocomial infections, tumour diseases and various forms of immuno-suppression initiated alternative methods in immunotherapy. Immunostimulation at the non-specific defense level has first been noticed as "side effects" of vaccination. Today it should be differentiated between substitution of the immune system with cytokines and induction of the non-specific defense repertoire mimicking natural antigen contact that is called paramunization. Advantages and disadvantages of both methods are discussed. In vitro as well as in vivo experiments with poxviruses document safety and efficacy of purified and inactivated virus particles in paramunization protocols. The main stimulative components of the poxvirus particles are located in the envelope of the virions. Poxvirus-induced stimulation of non-specific defense reactions is likely to have remote effects on the quality of further antigen processing. Besides the induction of a high short-term alertness in the primitive immune system paramunization may efficiently influence ongoing specific responses, e.g. immunoglobulin isotype selection. Therefore the term paramunization should not be used to characterize a separate part of the immune system, however, for didactic reasons it will facilitate the understanding of principles of the immune system.

Animals↗

[The salivary content of Streptococcus mutans in Basel children and adolescents].

The search for a feasible method to estimate the caries risk in humans is going on since several years. Such risk assessments should especially be performed in persons clinically suspected to be at risk. The quantitative determination of Mutans Streptococci (SM) in whole saliva has been proposed to serve as a measure of the caries risk. SM tests have been performed in a group of 5743 5 to 16 year old children and adolescents living in the city of Basle in 1991. Levels of SM in whole saliva were correlated with caries prevalence both in the deciduous and permanent teeth. SM counts have become a valuable diagnostic tool for the early identification of children at risk for caries development. SM tests have equally become a helpful pedagogic instrument used to motivate patients for individual prophylactic activities.

Adolescent↗

Photosynthetic reaction center genes in green sulfur bacteria and in photosystem 1 are related.

Oxygenic photosynthesis of chloroplasts and cyanobacteria involves two photosystems, which originate from different prokaryotic ancestors. The reaction center of photo-system 2 (PS2) is related to the well-characterized reaction center of purple bacteria, while the reaction center of photosystem 1 (PS1) is related to the green sulfur bacteria, as is convincingly documented here. An operon encoding the P840 reaction center of Chlorobium limicola f.sp. thiosulfatophilum has been cloned and sequenced. It contains two structural genes, coding for proteins of 730 and 232 amino acids. The first protein resembles the large subunits of the PS1 reaction center. Putative binding elements for the primary donor, P840 in Chlorobium and P700 in PS1, and for the acceptors A0, A1, and FeS center X are conserved. The second protein is related to the PS1 subunit carrying the FeS centers A and B. An adjacent third gene, not belonging to the reaction center, encodes a protein related to dolichyl-phosphate-D-mannose synthase from yeast. The different origins of PS1 and PS2 are discussed.

Amino Acid Sequence↗

The photosystem I-like P840-reaction center of green S-bacteria is a homodimer.

An operon encoding the P840 reaction center of Chlorobium limicola f.sp.thiosulfatophilum has been cloned and sequenced. It contains two structural genes coding for proteins of 730 and 232 amino acids. The first protein resembles the large subunits of the Photosystem I (PS I) reaction center. Putative binding elements for the primary donor, P840 in Chlorobium and P700 in PS I and for the acceptors A(o), A(1) and FeS-center X are conserved. The second protein is related to the PS I subunit carrying the FeS-centers A and B. Since all our efforts to find a gene for a second, large subunit failed, the P840 reaction center probably is homodimeric.

Amino Acid Sequence↗

Natural killer (NK) activity of porcine blood lymphocytes against allogeneic melanoma target cells.

Allogeneic PM/86 melanoma cells of Munich Troll miniature swine have been used for the demonstration of porcine peripheral blood NK cell activity. Compared with the specific lysis of xenogeneic K562-, U937- and Vero-target cells, NK cell-mediated cytotoxicity (NK-CMC) against PM/86 melanoma tumor cells was significantly lower in a 16 h chromium release assay. The target cell susceptibility to peripheral blood NK-CMC of both adult Troll miniature swine and German Landrace sows was very similar. Cold target inhibition assays revealed the allogeneic PM/86 melanoma cells to be the most powerful inhibitors of NK-CMC. Nylon wool non-adherent lymphocytes produced interferon (IFN)-alpha in different quantities upon contact with NK susceptible target cells. The NK effector cells could be stimulated to a higher lytic activity against all susceptible targets by a moderate dose of natural human interleukin-2 (nhuIL-2). The role of NK-CMC in melanoma tumor rejection and/or prevention of metastases is yet unknown in swine although porcine melanoma serves as a good model for the disease in man.

Animals↗