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Biomedical subjects

M Babé

Publications and source records attributed to M Babé.

9 recordsLinked to original sources
PubMed1987

[Perfusion of the dog tibia isolated for the study of mineral metabolism].

A method for perfusion of isolated canine tibia is described. With this procedure the influence of different albumins on the uptake of tritiated 25-hydroxyvitamin D3 (3H-25(OH)D3) by the tibia has been studied. Bovine serum albumin at 1% concentration seems to be optimum for this system. This experimental approach seems to be useful for the study of mineral metabolism.

Animals↗
PubMed1984

[Determination of 25-hydroxycholecalciferol in serum].

A precise specific method for measuring 25-hydroxycholecalciferol (25-OH CC) in 2 ml of human serum is described. It includes extraction with acetonitrile, separation with Sep-Pak C-18 cartridges, purification through HPLC and a further quantitation by means of a protein-binding assay. An exhaustive study of this protein-binding method has been performed. The sensitivity of the protein-binding assay is 20 pg/tube and the inter and intra-assay coefficients of variation are 9.6% and 8.7% respectively. The precision of the overall process has been assessed by calculating the inter and intra-assay coefficients of variation, 15.5 and 10.4% respectively. Mean serum value of 25-OH CC in normal subjects (18-50 years old, during spring) is 11.2 +/- 5.5 ng/ml.

Acetonitriles↗
PubMed1983

24,25-dihydroxyvitamin D3 in serum: sample purification with Sep-Pak C-18 cartridges and liquid chromatography before protein-binding assay.

We describe a precise, specific method for measuring 24,25-dihydroxyvitamin D3 in human serum. A 2-mL serum sample is extracted with acetonitrile and passed through a Sep-Pak C-18 cartridge. The sample is further purified by "high-performance" liquid chromatography under isocratic conditions on a normal-phase column (Radial-Pak silica-gel cartridge), then subjected to a protein-binding assay. The mean concentration of 24,25-dihydroxyvitamin D3 in serum from 22 normal adults (measured during the spring) was 2.9 micrograms/L (SD 1.9, range 6.3-0.42 microgram/L). The intra-assay CV was 7.7%, the interassay CV 11.2%. Purification of the sample with Sep-Pak C-18 and liquid chromatography on normal plus reversed-phase columns leads to a mean value of 3.4 micrograms/L (SD 1.6 micrograms/L, n = 12), not significantly different from results with our method.

24,25-Dihydroxyvitamin D 3↗
PubMed1980

A C-terminal radioimmunoassay for human parathyrin.

Several features concerning the parathyrin (PTH) radioimmunoassay (RIA) in human serum have been considered. The bovine hormone (bPTH) was used as standard and to obtain the radioiodinated tracer and the antisera employed exhibited a C-terminal specificity. A modification of the classical Greenwood and Hunter method was used to label bPTH, based on the sequential addition of small amounts of Chloramine-T to the solution containing the hormone to prevent ovariodination. The purification by Quso G32 followed by a gel-filtration step, produced a suitable tracer for a highly sensitive RIA. Due to unespecific effects from serum in the incubation medium, serum concentration was rendered uniform in all of the assay tubes prior to the addition of the separation mixture (charcoal-dextran). The greatest sensitivity reached was 16 pg of bPTH in the incubation mixture with Ch 4M antiserum. This antiserum allows detection of immunoreactive PTH in the majority of normal subjects and has applicability to a wide range of bone disturbances.

Humans↗