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Biomedical subjects

M Bakry

Publications and source records attributed to M Bakry.

18 recordsLinked to original sources
PubMed1994

Small round cell tumor with divergent differentiation: cytologic, histologic, and ultrastructural findings.

A case study of desmoplastic small round cell tumor with divergent differentiation in a 13-yr-old boy is presented. Clinical, cytologic, histologic, immunohistochemical, and ultrastructural findings are reported. There was a close correlation between the various histologic and cytologic patterns. Immunohistochemical studies revealed strong positivity of many of the tumor cells for cytokeratin, vimentin, and desmin. Ultrastructurally, some of the tumor cells contained aggregates of cytoplasmic intermediate filaments. Fine-needle aspiration biopsy may suggest the diagnosis of desmoplastic small round cell tumor. Confirmation of the diagnosis may be provided by immunohistochemistry and electromicroscopy.

Abdominal Neoplasms↗
PubMed1993

Fine-needle aspiration biopsy of Langerhans histiocytosis (histiocytosis-X).

A series of 14 fine-needle aspiration biopsies (FNAB) from histologically proven cases of histiocytosis-x (Hx) were reviewed. The smears revealed a variable mixture of Langerhans cells, eosinophils, macrophages, polymorphonuclear cells, and giant cells. Based on the predominant cells present, the cases were further categorized as Langerhans cell predominant (nine cases), eosinophil predominant (two cases), and macrophage predominant (three cases). Langerhans cells were usually polygonal without significant evidence of phagocytosis and frequently contained indented nuclei. In three cases, several Langerhans cells showed prominent dendritic processes. Electron microscopy in 10 cases revealed Langerhans cells with varying degrees of morphologic differentiation. All cases studied revealed Birbeck granules. These findings indicate that FNAB may be an effective technique for diagnosing Hx.

Adolescent↗
PubMed1992

Fine-needle aspiration biopsy of pediatric neoplasms: correlation between electron microscopy and immunocytochemistry in diagnosis and classification.

A series of fine-needle aspiration biopsies performed in 635 children were reviewed. The diagnoses rendered in these patients included malignant lymphoma in 139 (21.9%); Hodgkin's disease, 25 (3.9%); neuroblastoma, 58 (9.1%); Wilms' Tumor, 37 (5.8%); Ewing's sarcoma, 32 (5.0%); rhabdomyosarcoma, 25 (3.9%); retinoblastoma, 22 (3.5%); leukemia infiltrate, 33 (5.2%); and miscellaneous tumors, 52 (8.2%). In 171 patients (26.9%), the biopsy was nondiagnostic. The cytomorphological characteristics of these lesions are briefly described and illustrated. Salient morphological features are further correlated with histological and ultrastructural appearances. Immunocytochemical patterns of these tumors are also discussed briefly.

Biopsy, Needle↗
PubMed1992

Electron microscopy of fine-needle aspiration biopsy specimens: a brief review.

The cellular sample obtained by fine-needle aspiration biopsy is usually small and therefore requires extreme care during processing for electron microscopy. The most significant technical problem is due to contamination of the sample by red blood cells, which tend to dilute the samples. Red blood cells in these samples may be removed prior to processing by either using Bovine serum albumin as a gradient or by filtration of the specimen by a nylon sieve. Experience at our institution with the use of electron microscopy for interpretation of fine-needle aspiration biopsy samples is briefly reviewed.

Biopsy, Needle↗
PubMed1992

Fine-needle aspiration biopsy diagnosis of rhabdomyosarcoma: cytologic, histologic, and ultrastructural correlations.

A series of 15 cases of rhabdomyosarcoma diagnosed by fine-needle aspiration biopsy (FNAB) and confirmed by histopathology is reviewed. Cytologically, the tumors were composed of a variable mixture of cells, which according to the degree of differentiation were categorized as early, intermediate, or late rhabdomyoblasts. Histologically, the tumors were divided into embryonal 9, monomorphic round cell 4, and alveolar rhabdomyosarcoma 2. Comparison of histological and cytological features revealed that embryonal types were composed mainly of early rhabdomyoblasts. Recognition of these patterns may be helpful in FNAB diagnosis of rhabdomyosarcoma.

Adolescent↗
PubMed1991

Fine-needle aspiration biopsy diagnosis of malignant rhabdoid tumor of the kidney.

A series of six fine-needle aspiration biopsies from three patients with malignant rhabdoid tumor of the kidney is reviewed. The smears revealed round, polygonal, and irregularly shaped cells with large nuclei and prominent nucleoli. Some of the tumor cells contained light pink to purple cytoplasmic inclusions that correlated with the eosinophilic inclusions seen in histopathologic sections and filamentous cytoplasmic aggregates noted on ultrastructural examination. Diagnosis of malignant rhabdoid tumor of the kidney may be suggested from aspiration biopsy smears; however, further confirmation of the diagnosis by histologic or ultrastructural examination is desirable.

Biopsy, Needle↗
PubMed1991

Diagnosis of cutaneous leishmaniasis by fine-needle aspiration biopsy: report of a case.

A case of cutaneous leishmaniasis diagnosed by fine-needle aspiration biopsy is presented. The cytologic smears revealed lymphocytes, plasma cells, and macrophages. Large numbers of Leishmania organisms were present within the macrophages and in the intercellular spaces. Part of the material was used for a microbiologic culture, which revealed large numbers of promastigotes. These were studied by light, transmission, and scanning electron microscopy.

Adult↗
PubMed1991

Fine-needle aspiration biopsy of papillary thyroid carcinoma: cytologic, histologic, and ultrastructural correlations.

Aspiration smears from a series of 21 papillary thyroid carcinomas were studied and a number of morphologic features correlated with the histologic and ultrastructural patterns. These included types of papillary structures, metaplastic cells, grooved and lobulated nuclei, optically clear nuclei, intranuclear inclusions, and macrophages. Generally there was a close correlation between histologic and cytologic findings. The numbers of macrophages and metaplastic cells appeared to be exaggerated in aspiration smears as compared with those seen on histologic sections. Optically clear nuclei were not seen on the air-dried cytologic smears. Grooved nuclei were somewhat difficult to recognize in the aspiration smears, although lobulated nuclei were identified easily. We consider the latter to be a significant feature in the diagnosis of papillary carcinoma. Ultrastructurally grooved nuclei showed a deep linear indentation of the nuclear membranes. Lobulated nuclei were characterized by multiple indentations that divided the nucleus into several lobules.

Biopsy↗
PubMed1990

Fine-needle aspiration biopsy of seminoma and dysgerminoma: cytologic, histologic, and electron microscopic correlations.

Cytomorphologic features in a series of fine-needle aspiration biopsies from eight patients with seminoma or dysgerminoma were reviewed, and the findings were further correlated with histopathologic and ultrastructural appearances. Three cell types were encountered. Type I was least differentiated and least frequent. Type II was most frequent and had well-developed cytoplasm rich in organelles. Type III was similar to type II but also contained abundant cytoplasmic glycogen. In cytology smears, type III cells contained large punched-out cytoplasmic vacuoles corresponding to the areas of glycogen deposition. The background contained a variable mixture of inflammatory cells including lymphocytes, plasma cells, epithelioid cells, and epithelioid granulomas. The significance of these findings in the diagnosis of seminoma or dysgerminoma and the distinction from other germ-cell and non-germ-cell tumors is briefly discussed.

Adolescent↗
PubMed1990

Fine-needle aspiration biopsy diagnosis of endodermal sinus tumor: histologic and ultrastructural correlations.

Fine-needle aspiration biopsies from five patients with endodermal sinus tumors (ESTs) were reviewed, and the findings were correlated with histologic and ultrastructural appearances. In the aspiration smears, two types of tumor cells were seen, forming clusters of variable sizes. Type A cells had distinct cell borders, and their cytoplasm contained only occasional vacuoles. Type B cells had ill-defined cell borders and formed syncytial clusters; their cytoplasm was characterized by large numbers of rounded vacuoles. The background contained patches of mucoid material and macrophages with foamy cytoplasm. Eosinophilic hyaline cytoplasmic bodies and irregular deposits of intercellular basement membrane-like material were recognized easily in aspiration smears. These features correlated well with histologic and ultrastructural appearances. The significance of these findings in the fine-needle aspiration biopsy diagnosis of EST and its distinction from other germ-cell and non-germ-cell tumors is discussed.

Adult↗
PubMed1988

Aspiration cytology of retinoblastoma: light and electron microscopic correlations.

A series of 16 cases of retinoblastoma diagnosed by fine-needle aspiration biopsy (FNAB) and confirmed by histologic examination is reviewed, and the salient cytomorphologic features are described. Two types of cells were encountered in the aspiration smears; type I cells were undifferentiated while type II cells showed more differentiation and frequently revealed cytoplasmic processes that are probably indicative of early photoreceptor differentiation. Flexner-Wintersteiner rosettes characteristic of retinoblastoma were found in 10 of 16 cases. These findings were further correlated with ultrastructural examination of the tumors in nine cases. It is concluded that the presence of rosettes and type II cells with cytoplasmic processes are the two features that are most helpful in the FNAB diagnosis of retinoblastoma.

Biopsy, Needle↗
PubMed1988

Aspiration cytology of neuroblastoma: light microscopy with transmission and scanning electron microscopic correlations.

Fine-needle aspiration biopsies from three patients with neuroblastoma were studied by light microscopy, and the morphologic findings were correlated with those from transmission and scanning electron microscopy. Light microscopic examination of the aspiration smears from all three cases revealed small and large round cells with variable numbers of intertwining cytoplasmic processes. Transmission electron microscopy confirmed the light microscopic finding of cytoplasmic processes; in addition, it revealed the presence of other diagnostic morphologic features, including neurosecretory granules, microtubules, and synaptic cell junctions. Scanning electron microscopy demonstrated that the tumors were composed of a mixture of undifferentiated round cells and more differentiated cells with long cytoplasmic processes. The morphologic spectrum of these processes and their interrelationships with one another and with other cells could be studied in detail. These findings indicate that scanning electron microscopy may be used effectively in the morphologic evaluation and pathologic diagnosis of neuroblastoma.

Biopsy, Needle↗
PubMed1986

Aspiration cytology of neuroblastoma. Light and electron microscopic correlations.

Fine-needle aspiration biopsy (FNAB) smears from 16 cases of neuroblastoma are reviewed, and various cytomorphologic features helpful in diagnosing this tumor are described. Cytoplasmic processes were present in ten cases, and pseudorosettes in three cases. Electron microscopic study of FNAB samples revealed that all diagnostically important features, such as cell processes, neurosecretory granules, and synaptic cell junctions, could be recognized. These findings indicate that FNAB studied by light and electron microscopy is an effective method for diagnosing neuroblastoma.

Biopsy, Needle↗
PubMed1986

An improved technic for processing aspiration biopsy for electron microscopy.

This article describes a technic for processing aspiration biopsy samples for electron microscopy. Red blood cells present in these samples were separated from the tissue fragments with the use of a nylon sieve. The tissue fragments were pelleted and processed for electron microscopy in a routine manner. An analysis of 19 cases processed in this manner revealed that this separation technic is highly effective and results in a significant improvement in cellular yield.

Biopsy, Needle↗
PubMed1985

Fine-needle aspiration biopsy diagnosis of round cell malignant tumors of childhood. A combined light and electron microscopic approach.

A series of fine-needle aspiration biopsies (FNAB) from 76 children with round cell malignant tumors was studied by both light and electron microscopy. In 14 cases the material submitted for electron microscopy was inadequate, whereas in the remaining 62 biopsies a definitive diagnosis could be made. These included 29 malignant lymphomas, 8 neuroblastomas, 12 Ewing's sarcomas, 3 metastatic retinoblastomas, 3 Wilms' tumors, 3 rhabdomyosarcomas, and 4 unclassified tumors. The results from this study indicate that FNAB studied by light and electron microscopy can be effective in diagnosing small cell tumors in children.

Biopsy, Needle↗
PubMedDate not supplied

Malignant rhabdoid tumor of the kidney presenting as intraocular metastasis.

A newborn infant presented with intraocular tumor that was clinically diagnosed as retinoblastoma and treated by exenteration of the globe. Within a few weeks however, a large abdominal mass was noted, which was found to involve the left kidney and right lobe of liver. Pathologic evaluation of the intraocular mass as well as fine-needle aspiration biopsy of the abdominal mass revealed features of malignant rhabdoid tumor. To our knowledge this is the first case of malignant rhabdoid tumor of the kidney with such an unusual clinical presentation.

Biopsy, Needle↗