A method for selecting the coding DNA for a protein of known sequence. II. Use of obligatory restriction sites.
A method for selecting the coding DNA of a protein of known sequence in a library of chimeric plasmids constructed with cDNA is described. It is based on the prediction of obligatory restriction sites within the searched cDNA. A partially purified probe can be obtained by taking advantage of the occurrence of two such obligatory sites. Another suggested possibility is to combine one obligatory restriction site and a specific synthetic oligodeoxynucleotide used as primer in classical reverse transcription methods.
Amino Acid Sequence↗