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Biomedical subjects

M Bergeret

Publications and source records attributed to M Bergeret.

45 records · Page 3Linked to original sources
PubMed1992

Absence of intrathecal synthesis of some interferon-alpha subtypes in bacterial meningitis.

Sixty-five cerebrospinal fluid (CSF) samples from 54 infants and children with bacterial meningitis were analyzed for the presence of interferon (IFN)-alpha with a biologic assay. Of the 65 samples, 3 were positive (2-4 IU/mL) and only 1 of 56 collected early was at 4 IU/mL. These results suggest that some subtypes of IFN-alpha already reported as present in viral infections of the central nervous system are not detected in bacterial meningitis by our IFN assay. This difference may be helpful in differentiating bacterial from viral infections and also in evaluating the quality of the virologic investigation; moreover, the rarity of IFN-gamma in CSF in bacterial meningitis needs further investigation to understand its role in the pathogenesis of the disease.

Child↗
PubMed1991

[A rapid bacteriologic method for the diagnosis and surveillance of nosocomial pneumopathies].

The authors report a rapid quantitative culture of endotracheal aspiration samples in mechanically ventilated children. There was a good correlation (94.7%) between clinical and radiographic features of pneumonia and a cutoff point of 10(5) cfu.ml-1. The sensitivity of this rapid quantitative culture was 85% when compared with usual quantitative culture.

Bacteriological Techniques↗
PubMed1986

Isolation of two strains of Kingella kingae associated with septic arthritis.

Two new cases of infection, a presternal abscess and a spondylodiscitis caused by the recently classified bacterium Kingella kingae, are reported. The main bacteriological characteristics and the susceptibility of the two isolates to antimicrobial agents are described. The pathology of K. kingae, particularly among children, is reviewed.

Abscess↗
PubMed1983

Interferon effect on cytolytic T lymphocytes in a single cycle assay.

In this study we analyse cell-mediated cytotoxicity by the use of 51chromium release and the killer cell enumeration assay. The latter enabled us to estimate cell-mediated cytotoxicity in a single cycle, which confirms the protective effect of interferon (IFN) after target cell treatment. This anti-cytolytic resistance is detected best by microassociation when effector and target cells are separately treated with IFN and associated thereafter. We suggest that, in inflammatory areas, enhanced cytotoxic activity of the IFN-treated effector cells is only operative before the establishment of protection in the targets, which is somewhat slower and appears in about 18 hr. Resistance of the targets could be at least partly attributed to the incapacity of effector cells to bind to them.

Animals↗
PubMed1980

[Protective effect of interferon on the target cells of cytotoxic lymphocytes].

Interferon treatment of sensitized T lymphocytes enhances cytotoxicity against target cells. On the contrary, the same treatment of the target cells alone protects them, whatever the cytotoxicity of effector cells might be. We suggest that the cells having once survived contact with cytotoxic T lymphocytes could become resistant to any new attack by the same cells.

Animals↗
PubMed1980

Protective effect of interferon on target cells exposed to cellular cytotoxicity.

In order to explore by immunological methods the interferon-induced cell membrane modulation, the effect of interferon in parental and monkey-mouse hybrid cells was studied in the presence of sensitized lymphocytes from isologous mice. After primary immunization to the hybrids, both parental interferons increased the lymphocyte-induced specific cytotoxicity in the same hybrid cells used as targets. In addition, a significnt increase of spontaneous lysis due to the two interferons was observed. In contrast, after secondary sensitization, in spite of the higher specific cytotoxicity induced by the lymphocytes, the same interferon preparation significantly protected the cells. These results are probably related to a greater number of sensitized effector cells after secondary sensitizationn. It is likely that, although interferon increases the affinity of sensitized T lymphocytes for the target cell membrane, in case of unsuccessful encounters, the target cells become resistant to new hits. Interferon could augment the number of such immune cells.

Animals↗
PubMedDate not supplied

Immunomodulatory effects of arginine butyrate.

We have studied the effect of arginine butyrate on T cell and macrophage functions. When target cells are treated with this substance, they become resistant to T cell-mediated cytotoxicity, as detected by the chromium assay. In contrast, when effector T cells are treated, the cytotoxicity seems to be augmented. Peritoneal macrophages incubated with butyrate are increasingly adhesive to substrate. After in vivo treatment, spleen derived macrophages show an augmented cytostatic capacity in the presence of L1210 cells and an enhanced phagocytic activity for IgG-coated erythrocytes. To sum up, the overall effects of butyrate salts on different immune functions are somewhat reminiscent of that of interferon. It is likely that these immune effects contribute, at least in part, to explain its antitumor properties observed in grafted tumors in mice.

Adjuvants, Immunologic↗