A whole blood FK 506 assay for the IMx analyzer.
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Autoanalysis↗
Biomedical subjects
M Bergmann
Publications and source records attributed to M Bergmann.
[The CNS in AIDS and in asymptomatic HIV positive patients].
Neuropathological investigations were carried out on 166 autopsies of HIV-seropositive patients, with and without AIDS. Opportunistic infections and lymphomas were present in about 50% of cases; 65 patients were bearers of HIV-encephalopathy. HIV core protein p24 was detected in few mono- and multinucleated macrophages (HIV-cells), only in cases with HIV-encephalopathy. In the CNS of HIV-positive, asymptomatic patients no histological or immunohistochemical abnormalities were seen. These findings let suppose that AIDS-Dementia is a result of a late infiltration of HIV-infected macrophages from the bloodstream into the brain and not due to an impairment of neuronal or glial cells infected by HIV in the early stages of the disease.
AIDS Dementia Complex↗
[Progressive multifocal leukoencephalopathy (PML) in AIDS: morphological and topographical characteristics].
15 autopsy cases of PML in AIDS are presented. Frequent findings were: Very large, confluent PML-lesions (7 cases), lesions with prominent necrotic features (10 cases) and with marked perivascular mononuclear infiltrates (6 cases). In 9 cases additional PML-foci were seen in the cerebellum and/or the brain stem, and in 1 case there was even an exclusive involvement of those structures. Such characteristics regarding severity and topography of PML in AIDS, may be due to additional toxic factors. In 7 cases HIV-encephalopathy with multinucleated macrophages was present.
Acquired Immunodeficiency Syndrome↗
Annulated gap junctions in the rat neural tube.
Annulated gap junctions have been studied electron microscopically in normal rat embryo neural tube on day 12 of gestation. Localization, size and content of annulated gap junctions were characterized. Like in other tissues annulated gap junctions are thought to be involved in a degradation cycle of gap junctions.
Animals↗
[Therapy-induced cerebro-renal oxalosis?].
Report on three cases of a cerebro-renal oxalosis. The clinical picture was characterized by subacutely appearing coma and cerebral paroxysms. Death occurred due to break-down of cerebral regulation. In all three cases the post-mortem examination showed massive subendothelial depositions of double refractive crystals in the cerebral vessels, in particular in the stem ganglia regions and in the renal tubuli. The crystals showed several features typical for calcium oxalate. The clinical picture appears as an infrequent complication within intensive medicine, in connection with parenteral administration of xylit. Differential diagnosis, pathogenesis and possible cofactors are discussed.
Aged↗
[Clinical, infrared spectroscopic and chemical analysis of bile duct and gallbladder stones].
By means of concrements, which were obtained endoscopically by extraction from the bile duct, and of gallstones, which were given the patient by the surgeon on the occasion of the gallbladder operation, we performed stone analyses with the aid of infrared spectroscopy and compared them correlatively with clinical and case history data. The paper focussed on the detection of the stony structure of choledochal concrements, separated into stone core and stone mantle. A method for the reliable differentiation of stones left (residual stones) from new formations (recurrent stones) in the bile ducts has not been possible to date. Thus, the burning question of the surgeon cannot be answered with certainty. However, there is no doubt about surgical suture material as incrustation core for a pigment stone in terms of a true recurrent stone, which poses a real challenge to biliary tract surgery.
Bile Pigments↗
[Primary intracerebral non-Hodgkin's lymphoma].
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Adult↗
[Morphologic findings in congenital syphilis].
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Abnormalities, Multiple↗
[Selected psychoneurotic-somatic disorders and diseases of the digestive tract. An integrated unitary concept].
On the occasion of the 10th anniversary of the inauguration of our new, modern Department of Internal Medicine in Jena it is our intention to characterize, at least roughly, the contribution to its profile that has been made since its institutionalization. In this context, problems that were related to the main tasks in patient care and to the character of the division of general internal medicine had to be picked out from the multitude of services which have been provided by the staff of the division for the benefit of our citizens. As exemplified by two problematic syndromes of the digestive tract, we wished to demonstrate that a non-integrative dualistic medical practice in diagnostics and therapy is not suitable to produce the necessary qualitative breakthrough in our public health service. Only by stepwise and limited diagnostics can a balanced cost-to-benefit ratio be advocated for the patient and the society. Our scientifically oriented mode of prescribing therapeutic measures for the citizens in our public health institutions is intended to harmonize, more than ever before, quality with economics, as the international trend of growing costs in public health services also holds true for the GDR. These few suggestions will have outlined at least first steps in the right direction.
Adult↗
Stage-dependent appearance of sulfhydryl oxidase during spermatogenesis in the testis of rat and hamster. An immunohistochemical study.
Sulfhydryl oxidase (SOx), an enzyme that catalyzes the oxidation of sulfhydryl compounds, appears in the spermatogenic cells of rat and hamster testes in a stage-dependent manner. It first appears in pachytene spermatocytes at stage I in both the animal species studied. SOx immunoreactivity is associated with mitochondria of these cells. The fate of such mitochondria is species-dependent. In rat, the immunoreactive mitochondria aggregate during maturation phase and are retained in the residual bodies. Spermatozoa free of SOx are released into the lumen. On the other hand, in hamster, the immunoreactive mitochondria arrange themselves around the midpiece of spermatozoa. In such a case, residual bodies lack SOx. The appearance of SOx coincides with the appearance of LDH-X in the spermatogenic cells. Like many other proteins such as LDH-X, RSA-1 and cytochrome ct, SOx provides yet another example of differential gene activation associated with a developmental process of gametes.
Animals↗
Primary liposarcoma of the jejunum.
The rare case of a primary liposarcoma of the jejunum in a 52-year-old man is described. The main tumor mass was located within the submucosa and caused chronic subileus by subtotal obstruction of the jejunum. The histology showed a mixed type liposarcoma. In the main mass features of well differentiated lipoma-like and sclerosing liposarcoma predominated. In the tumor periphery they were combined with areas of pleomorphic liposarcoma, consisting of tightly packed giant lipoblasts. After an incomplete resection well differentiated sclerosing and pleomorphic liposarcoma recurred within the intestinal wall and in the mesentery near the primary anastomosis. The unusual primary site and pattern of spread of the tumor are discussed and the literature is reviewed.
Humans↗
[Paraneoplastic encephalomyelitis].
A case of parancoplastic encephalomyelitis detected in a 60 year old man with clinical signs of a polyradiculitis and anterior horn lesion is reported. Death occurred after 5 months due to cardiorespiratory insufficiency. On autopsy, an occult small cell carcinoma of the lung with metastases in the regional lymph nodes was found. The neuropathological examination revealed a polioencephalomelitis which primarily involved the limbic cortex, brain stem, spinal cord and posterior root ganglia. No virus material could be detected with immunhistochemical methods and in situ hybridisation. The inflammatory infiltrate was mainly composed of T-cells and to a lesser extent B-cells and mature macrophages. Literature and hypotheses concerning pathogenesis are reviewed.
Brain↗
Distribution pattern of testicular sulphydryloxidase immuno-activity in the djungarian hamster (Phodopus sungorus) during photoperiodically induced involution and recrudescence.
The distribution pattern of testicular sulphydryloxidase (SOx) immuno-activity was investigated in the djungarian hamster during photoperiodically induced testicular involution and recrudescence. SOx immuno-activity, indicating functional integrity of labelled cells, did not change in pachytene spermatocytes and spermatids as long as these cells were present in the seminiferous epithelium. Its disappearance coincided with the degeneration of spermatocytes in phases IV and V of involution and reappeared during recrudescence, when the first spermatogenic wave had reached the pachytene stage. In tubules at phase VI of involution (showing maximal regression), the apical cytoplasm of Sertoli cells showed immuno-activity. This immuno-activity disappeared during recrudescence prior to the differentiation of pachytene spermatocytes. Changes in SOx immuno-activity resembled those of lactate dehydrogenase-X (LDH-X) in photo-inhibited testes or during puberty, indicating a close functional relationship which still remains to be elucidated. The data suggest that the hamster exposed to different photoperiods can be used as a suitable model to study the relationship between testicular morphology and function in different states of gonadal activity.
Animals↗
Long-term echocardiographic examinations in chronic hemodialysis patients substituted with recombinant human erythropoietin.
Cardiomegaly and impaired cardiac function induced by renal anemia are frequent findings in patients with chronic renal failure. The present investigation was performed to study the cardiac effects of a therapy with recombinant human erythropoietin in patients on maintenance hemodialysis. Echocardiographic examinations showed a decrease in cardiac size and left-ventricular mass continuously over 12 months of effective erythropoietin substitution. Cardiac output was reduced, and ejection fraction and myocardial contractility increased. The development of aggravation of arterial hypertension did not counteract the beneficial effects of erythropoietin on cardiac performance.
Cardiomegaly↗
[Striato-nigral degeneration (SND): a multisystem atrophy?].
Two cases of striato-nigral degeneration are reported. In case 1 the female patient showed a Parkinson syndrome, cardiac arrhythmias and vasomotor disturbances. Morphologically mainly the putamen and substantia nigra revealed severe atrophic changes according to the restricted form of striato-nigral degeneration (SND). In case 2 there was a disorder of the upper and lower motor neuron in a female patient. Morphologically this case was an example of a multisystem atrophy with changes in the striato-nigral, olivo-ponto-cerebellar systems and spinal motor and autonomic neurons. These cases demonstrate the variability of the striato-nigral degenerations which are met with in two forms: a "pure" form as in case one and as a part of multisystem atrophies. This is underlined in a review of 69 literature cases, which also shows that SND, Shy-Drager syndrome and olivo-ponto-cerebellar atrophy represent very probably different varieties of one and the same degenerative process.
Aged↗
[Centroblast morphology: a morphometric evaluation by means of computer-aided image analysis].
In cytologic preparations, cell images from 30 cases of high grade malignant Non-Hodgkin's lymphoma (NHL) and 10 cases of tonsillitis have been analysed using a colour TV microscope system, high resolution scanning and image processing. Multivariate analysis of 30 cell features allowed satisfying recognition and discrimination of centrocytes, centrocyte-like centroblasts, ordinary centroblasts and multilobated centroblasts. In addition, according to their image analytical data, the ordinary centroblasts from NHL formed 5, and those from tonsillitis 4 well defined, homogeneous clusters. Even at simultaneous analysis, there was an essential overlap only between centroblast subtype 3 from NHL and subtype 9 from tonsillitis, but reliable discrimination of all the rest.
Computers↗
Neuroepithelial cell contacts in 12 days old rat embryo: normal and annulated gap junctions.
Neuroepithelial cells in the neural tube of 12 days old rat embryos make contact with each other by extended junctional complexes between their lateral membranes near the luminal surface. In this stage gap junctions are not numerous. Besides these "normal" gap junctions, especially in the upper part of the neuroepithelial cells, annulated gap junctions occur. They seem to originate by an endocytotic process of normal gap junctions with participation of cytoskeletal elements. The content of these annulated gap junctions mainly consists of ribosome-like particles in a high density, but larger particles may also occur. Annulated gap junctions sometimes reveal an intimate contact with the smooth endoplasmic reticulum or with the perinuclear cistern. If present in the perinuclear region the annulated gap junctions show membrane irregularities and other signs which are thought to mark steps of breakdown. Life-cycle and possible functions of the annulated gap junctions during neurulation need further investigation.
Animals↗
Automated recognition of cell images in high grade malignant lymphoma and reactive follicular hyperplasia.
In the past, the comparison of results of studies on malignant lymphomas has been biased by the use of different classifications of the diseases and to an even greater extent by subjective interpretation in the classification of the tumour cells. To overcome these short-comings, we have developed cytometric features specifically for automated recognition of cell images from high grade malignant non-Hodgkin's lymphomas (NHL) and reactive lymphoid lesions. This study used a colour TV-microscope system, high resolution scanning (13.3 pixel/microns), and image processing to study a total of 3600 lymphoid cells from 15 high grade malignant NHL and three tonsils. Sixteen out of 64 features, especially developed for image analysis in cytological preparations, have been evaluated. Because of a considerable overlap of all the single features, no feature on its own allows reliable discrimination. But, multivariant analysis of suitable feature combinations resulted in reliable identification and discrimination of the most frequently occurring cell types. We show that the lymphocytes, centrocytes, centroblasts, immunoblasts and lymphoblasts, as they are defined by subjective morphological criteria in the Kiel-classification of malignant NHL, also form distinctive subpopulations on the basis of their objective mathematical cell features. Furthermore, we have shown that there are distinctive differences between the lymphoma cells and their benign counterparts derived from reactive lymphoid lesions.
Diagnosis, Differential↗