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Biomedical subjects

M Beskid

Publications and source records attributed to M Beskid.

At least 19 recordsLinked to original sources
PubMed1992

Quinolinic acid: effects on brain catecholamine and c-AMP content during L-dopa and reserpine administration.

The catecholamine content in rat brain tissue was determined following the administration of quinolinic acid alone or combined either with L-dopa and decarboxylase inhibitor or reserpine. Quinolinic acid alone decreased the levels of dopamine and noradrenaline, as well as those of c-AMP, and increased those of adrenaline. Treatment with L-dopa/decarboxylase inhibitor reversed the suppressing effect of quinolinic acid on dopamine, but not on noradrenaline. Reserpine alone depleted the contents of dopamine, noradrenaline and adrenaline. It could be concluded from the effects of quinolinic acid and reserpine given together that quinolinic acid suppresses the depletion of amines induced by reserpine. It has been demonstrated that quinolinic acid leads to injuries of nerve-cell bodies in pars compacta of the substantia nigra and in the striatum. Quinolinic acid is a natural metabolite of tryptophan, normally occurring in the liver, kidney and brain (Wolfensberger et al. 1983; Moroni et al. 1984). This compound exhibits convulsant and neuron excitant properties (Stone et al. 1987). It induces a selective pattern of neuronal degeneration both at the site of intracerebral injection (Schwarcz et al. 1983; Stone et al. 1987) and after general (intracardiac) administration (Beskid and Markiewicz 1988). The ability of quinolinic acid to produce neurotoxicity was greater in the striatum than in other parts of the brain. This prompted us to study catecholamine and c-AMP levels in rat brain tissue following quinolinic acid and L-dopa administration, as well as the influence of reserpine on quinolinic acid action.

Animals↗
PubMed1979

Cyclic AMP mediated activity stimulation by metronidazole in rat liver.

In the homogenate of the liver biochemically examined 45 hrs after a single 250 mg dose of metronidazole administration, a 45% increase of c-AMP level was found as compared with controls. A marked proliferation of hepatic smooth endoplasmic reticulum was observed. In the light microscope numerous mitotic figures could be seen. No mitotic figures were present in the liver tissue of either treated or untreated animals examined 24, 72 and 120 hrs post administration of metronidazole. Two points seem of interest in the biologic properties of metronidazole: one is the participation of hepatic smooth endoplasmic reticulum in its metabolism, the other--the formation of active metabolites which may posses carcinogenous properties.

Animals↗
PubMed1979

Encephalitis congenita symptomatica toxica in experimental conditions.

The object of the study was the cerebral cortex of newborn rat. Tissue samples for examination were collected from the 2nd to the 8th hour after birth. Experimental material was the tissue of control animals and newborns whose mothers had been treated with both ethanol and pyrazole throughout gestation, and also with either ethanol or pyrazole alone. Ethanol and pyrazole were administered by gastric tube, ethanol at doses of 8.0 g/kg body weight, pyrazole at doses of 36 mg/kg body weight. The results indicate that ethanol ingested by the mother during gestation inhibits cell maturation in the cerebral cortex, whereas the combined administration of ethanol and pyrazole produces a highly toxic action. Its morphological exponent are symptoms which support the diagnosis of encephalitis congenita symptomatica toxica.

Animals↗
PubMed1979

Histochemical and morphological evaluation of cerebral cortex of newborn rat in the course of joint ethanol and pyrazole administration.

The object of the study was the cerebral cortex of newborn rat. Tissue material was collected from the 2-nd to the 8-th hour after birth both from control animals and newborns whose mothers had been given both ethanol and pyrazole throughout gestation period, and also either ethanol alone or pyrazole. The drug were administered by gastric tube, in doses: ethanol 8.0 g/kg body weight, pyrazole 36 mg/kg body weight. Histochemical studies revealed variation in the intensity of reaction of the respiratory enzymes during examination of the cortex of newborns whose mothers had been fed with ethanol alone, or pyrazole alone. A strong inhibition of enzymatic reaction was observed in the examined cerebral cortex of newborns after a joint ethanol and pyrazole administration. Morphological studies showed an inhibition of maturing process of the cerebral cortex cells of animals treated with ethanol alone, whereas the cerebral cortex of animals whose mothers had been given both ethanol and pyrazole, presented symptoms allowing to diagnose encephalitis congenita symptomatica.

Animals↗
PubMed1979

C cell adenoma of the human thyroid gland.

A case of C cell thyroid adenoma in a 47-year-old female patient is described. The nodule showed progression over 34 years. The adenoma cells showed histochemical and ultrastructural properties characteristic for C cells. Histologic examination revealed no atypical features of nodule cells nor infiltration of capsule by neoplastic cells. In the period of 8 years following the excision there was neither recurrence nor metastases.

Adenoma↗
PubMed1979

Electron microscopic and ultracytochemical investigations of thyroid C cells in primary hyperparathyroidism.

The subject of study were C Cells of the human thyroid in course of primary hyperparathyroidism caused by the presence of a single adenoma. C cells were estimated by routine electron microscopic technique and by cytochemical cholinesterase (ChE) reaction according to Iwayama in electron microscopic technique. The presence of single C cells and of a small agglomeration of these cells, parafollicularily localized was found in the thyroid gland. An electron dense reaction product was noticed on membraneous structures of C cells of the thyroid.

Adenoma↗
PubMed1978

Ethanol toxic effect on the newborn rat liver.--Histochemical and electronmicroscopical investigations.

The object of the study was the liver of newborn rats. Specimens were taken from the 2nd to the 8th hour after birth. Tissue material was obtained from control animals and the newborns whose mothers had been ethanol fed throughout gestation period. 40% ethanol was administered in doses of 8.0 g/kg weight, by gastric tube. In the newborn liver ethanol ingestion had led to significant accumulation of lipids, a strong acid phosphatase reaction and to a drop in succinic dehydrogenase activity. Histochemically, the intensity of alcohol dehydrogenase activity did not show any difference when the ethanol treated newborn liver was compared with controls. Ultrastructurally, the changes in the liver cells were expressed by a disappearance of the rough endoplasmic reticulum elements. Mitochondria were often swollen and distorted.

Acid Phosphatase↗
PubMed1978

Combined action of pyrazole and ethanol on a rat liver: histochemical and ultrastructural study.

The effect on a rat liver of combined administration of pyrazole and ethanol was studied histochemically and by electron microscopy. The study revealed strong hepatotoxic action of pyrazole combined with ethanol. Feeding with ethanol alone induces slight alterations in the liver, pyrazole alone affects the liver but slightly, whereas a combined administration of pyrazole and ethanol both by light and electron microscopy examination revealed a strong toxic action leading to severe damage of the liver cells including necrosis.

Acid Phosphatase↗
PubMed1978

Histochemical investigation of aldosterone-secreting cells adenoma of the adrenal cortex.

Two cases of adenomas of adrenal cortex were examined. Both cases presented clinical symptoms of autonomic hyperaldosteronism. Histologically one adenoma had a benign structure, the other a malignant one. An ultrastructural and cytochemical assay of the benign form suggests that it is composed of so called "hybrid cells". Histochemically the cells of both adenomas were characterized by a 3-beta hydroxysteroid and succinic dehydrogenase reaction, the presence of MAO and nonspecific esterases and in some single cells 17-beta hydroxysteroid dehydrogenase.

Adenoma↗
PubMed1977

Histochemical features of C-cell thyroid carcinoma.

Six cases of C-cell carcinoma of thyroid gland were studied applying histologic and histochemical methods as well as electron microscopy technique. A histologic feature of the carcinoma pattern was the diversity of pictures. Amyloid was commonly found in the carcinoma stroma. In some cells glycogen was present. Histochemically the carcinoma cells were marked by a strong activity of oxidative enzymes (GDH, SDH, LDH and MAO) as well as hydrolitic one (G-6-P and non specific enterases). Serotonin was found in two cases. An ultrastructural feature of carcinoma cells was the presence of cytoplasmic electron-dense, specific "endocrine type" secretory granules.

Amyloid↗
PubMed1975

Primary C-cell hyperplasia within a nodule of thyroid isthmus.

The electron microscopy method was used to study one case of primary C-cell hyperplasia in 'hot' thyroid nodule of isthmus. Hyperplasia of the light form of C cells was found within nodule tissue. It suggests that C-cell hyperplasia within the nodule as well as in normal thyroid tissue plays a role preceding C-cell carcinoma.

Adenoma↗
PubMed1975

Thyroid C-cells in normal and goitrous gland: a histochemical study.

16 cases of goiter were examined for C-cells and 4 cases with normal thyroid gland. C-cells identification was based on masked metachromasia. There was stated both in toxic, atoxic goitrous tissue two forms of C-cells. The first characterized by positive metachromatic reaction and weak argyrophinty, the second type of C-cells were orthochromatic and argyrophilic. Metachromatic C-cells characterized as a rule by high alpha glycero phosphate menadione reductase as well succinic dehydrogenase and cytochrome oxidase.

Calcitonin↗