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M Biasiolo

Publications and source records attributed to M Biasiolo.

24 records · Page 2Linked to original sources
PubMed1984

The polymorphism of transferrin by ultrathin-layer isoelectric focusing. Tf phenotypes and TfC subtypes in the population of Padua.

The distribution of Tf phenotypes in the population of Padua was investigated by ultrathin-layer isoelectric focusing. In our sample (n = 618) nine phenotypes, Tf C1, C2, C3, C3-1, C2-1, C3-2, C1B, C2B and C1D, were observed and the following frequencies calculated: TfC1 = 0.77837; TfC2 = 0.1804; TfC3 = 0.03641; TfB = 0.0040; TfD = 0.0008. These gene frequencies have been compared to those found in other populations. Analysis of 101 mother-child pairs was in agreement with an autosomal codominant mode of inheritance.

Child↗
PubMedDate not supplied

Problems in probability of paternity interpretation.

This paper discusses the statistical interpretation of blood group findings in paternity testing. As a consequence of the large number of systems now employed, high probabilities of paternity are usual and evaluation problems arise. The purpose of this investigation was to calculate the paternity probabilities for a sample of legitimate families with a true father compared with those obtained in some cases of non-excluded men chosen randomly from the population as the accused fathers for the same mother-child pairs. The calculations were based on Essen-Möller formula, derived from Bayes' Theorem. The blood group systems taken into account were ABO, Rh, MNSs, Kell-Cellano, P1, Duffy, Lutheran, Kidd, Gc, Hp, Gm, Km, Tf, alpha 1-AT, AcP, PGM1, AK, ADA, EsD, 6-PGDH and GLO-I. Applied together these give an exclusion probability of 97.32%. The results of probability of paternity for some mother-child-father triplets and its comparison with the chance of exclusion for the same mother-child pairs are reported.

Humans↗
PubMedDate not supplied

Tryptophan, copper and zinc in hair of healthy subjects. Correlation with differences in hair pigmentation.

Tryptophan, copper and zinc levels were determined in the hair of 300 healthy subjects divided by sex, age and hair colour. Sex influences tryptophan content in hair, the levels of this amino acid being higher in males than in females. Tryptophan is also higher in infancy (2-5 years) and in both males and females aging around 80 years and over. Hair colour also influences tryptophan levels, which increase from fair to black hair. Copper levels are similar in hair of both males and females, while those of zinc are higher in women. Age influences the distribution of these two metals in human hair. Copper contents in hair of males are higher at the age 20-40 years. In females, values decrease over the age of 60 years. Instead, zinc levels are higher between 20 and 60 years in males, and between 13 and 19 years in females. As regards hair colour, copper is slightly higher in black hair in males and in fair hair in females, and lower in white hair in both sexes. Zinc values appear to be higher in red and lower in white hair in males. In females they are higher in black hair.

Adolescent↗
PubMedDate not supplied

Effects of two different loading doses of L-tryptophan on the urinary excretion of tryptophan metabolites in rats, mice and guinea pigs. Correlation with the enzyme activities.

The effect of two different loading doses of L-tryptophan (0.5 and 1.0 g/Kg b.w.) on excretion of tryptophan metabolites and the relation to the enzyme activities were studied in rats, mice and guinea pigs. In rats there is no ratio between the dosage used and the levels of the metabolites excreted. Doubling the amount of tryptophan administered, a 5-fold increase in the elimination of the metabolites along the kynurenine pathway is obtained. The 1.0 g/Kg load provides a more complete pattern of the metabolites than with the 0.5 g/Kg b.w. load. Kynurenic acid, kynurenine and xanthurenic acid are the chief metabolites excreted. In mice, the urinary excretion of the metabolites is very low with both loads. In guinea pigs, xanthurenic acid is excreted in the highest amount and kynurenic acid and kynurenine also constitute the large fractions with both loadings. The load of 0.5 g/Kg b.w. is preferable to that of 1.0 g/Kg b.w. for not causing B6-deficiency. Liver tryptophan pyrrolase exists in two forms in rats, while in mice and in guinea pigs it is present only as holoenzyme. This enzyme is more active in rats than in the other two species of animals. Kynureninase activity is lower in guinea pigs, but it apparently correlated to the low levels of excretion of the metabolites following this step. Kynurenine aminotransferase is very active in rats and in mice, while it is apparently depressed in guinea pigs, in contrast with the high excretion of xanthurenic and kynurenic acids, that puts in evidence a B6-deficiency. The excretion of tryptophan metabolites and enzyme activities are better correlated in rats.

Animals↗
PubMedDate not supplied

Metabolites and enzyme activities involved in tryptophan metabolism in two different strains of mouse.

The urinary excretion of the tryptophan metabolites along the kynurenine pathway and the variations of enzyme activities involved in the degradation of tryptophan have been studied in two strains of mice. The total excretion of the metabolites was significantly higher in the strain of albino N.C.L. than in Swiss albino mice, in accordance with the higher activity of tryptophan pyrrolase, which was present only as holoenzyme. In both strains kynurenine, kynurenic and xanthurenic acids were excreted in larger amounts. However, the albino N.C.L. mice excreted a large amount of xanthurenic acid, not correlated with the slightly higher kynurenine aminotransferase activity observed in this strain. The very high excretion of this metabolite indicates that the load of tryptophan causes a B6-deficiency. Liver kynureninase activity was similar in both strains. Correlation between total urinary excretion of the tryptophan metabolites and enzyme activities appears in the same strain of mice, even though differences are present in different strains.

Animals↗