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M Bielaszewská

Publications and source records attributed to M Bielaszewská.

16 recordsLinked to original sources
PubMed1990

[Hemolytic-uremic syndrome--microbiological study].

In autumn 1988 in one locality of the North Bohemia region young children developed severe diarrhoea and the children were sent because of their serious clinical condition with the diagnosis of haemolytic-uraemic syndrome (HUS) to the Prague-Motol hospital. The probable aetiological agent which was detected was Escherichia coli producing verotoxin (VTEC)--026:H11, 026:H?, O157:H7, 05:H- and 01:H?. Biotyping and serotyping of the strains was made, the verotoxin level was assessed and their sensitivity to antibiotics was tested. Concurrently contacts were examined and suspect colonies of Escherichia coli were subjected to bio- and serotyping, however, none of the serovars found in the sick children was detected.

Bacterial Toxins↗
PubMed1990

[Campylobacter pylori and treatment of duodenal ulcer].

The authors subjected to endoscopic examination 90 patients with duodenal ulcers. From each patient they collected a bioptic specimen of the gastric antrum and duodenal bulbus for microbiological and histological examination. They found that for detection of C. pylori the microscopic examination was most useful. The presence of C. pylori in the gastric antrum was detected in 87% of the subjects and in the duodenal bulb only in 48%. The difference is significant at the 0.01 level. To 43 patients of the group Ranitidine was administered. The ulcer was cured within 4 weeks in 35 subjects. Ranitidine did not affect the presence of C. pylori and antral gastritis. After cure of the ulcer the authors administered Tarivid to 10 patients with persisting C. pylori positivity. The presence of C. pylori and antral gastritis were not affected by this treatment.

Adult↗
PubMed1989

[Microbiological aspects of infection with verotoxin-producing E. coli strains in children with the hemolytic-uremic syndrome].

The authors examined the faeces of five children with haemolyticuraemic syndrome for the presence of E. coli producing verotoxin (VTEC) and free verotoxin (VT). For detection of strains of serotype O157:H7 they used sorbitol MacConkey agar in combination with biochemical tests and typing of sorbitol negative strains. For detection of strains belonging to the other VTEC serogroups they used serotyping of 24 E. coli colonies from End media. VT was assessed in lysates and supernatants of cultures, on cell cultures of Vero cells, and the antigenic VT type was assessed by neutralization experiments. Strains corresponding as to serotype or O group to VTEC were detected in faeces of all five children. Two strains belonged to the serotype O157:H7 and had a typical biotype; another four strains belonged to serogroups 026 (2 strains), 05 and 01. All strains produced verotoxin, either VT1 or VT2 or both. In the faecal filtrates of all five children free VT was present. In conjunction with the haemolytic-uraemic syndrome in children in the CSSR E. coli producing verotoxin are found, incl. strains of serotype O157:H7, the detection of which was not described hitherto.

Bacterial Toxins↗
PubMedDate not supplied

Vero cytotoxin-producing strains of Escherichia coli in children with haemolytic uraemic syndrome and diarrhoea in Czechoslovakia.

The presence of verotoxin-producing strains of Escherichia coli (VTEC) was examined in six children with haemolytic uraemic syndrome and one child with haemorrhagic colitis. Stools were screened for strains of serogroup O157 on sorbitol-MacConkey agar for VTEC of other serogroups by serotyping. Verotoxin (VT) was tested on Vero cell monolayers: the antigenic variant of VT was assessed by neutralization experiments. Strains producing verotoxin 1 or verotoxin 2 or both were detected in the stools of all seven children. Three strains belonged to serogroup O157 (two of them to serotype O157:H7, one was non-motile) and another five belonged to serogroups O26 (two strains), O1, O5 and O18. The faeces of five children available for testing contained free VT. Production of VT was also examined retrospectively in 32 E. coli strains of serotype O26:H11 isolated from children with diarrhoea; eight (25%) of them produced moderate to high levels of verotoxin 1 despite several years storage in vitro. In conclusion, VTEC including strains of serogroup O157 seem to be an important cause of haemolytic uraemic syndrome, haemorrhagic colitis and diarrhoea in children in Czechoslovakia.

Bacterial Toxins↗
PubMedDate not supplied

Verotoxigenic (enterohaemorrhagic) Escherichia coli in infants and toddlers in Czechoslovakia.

The results of the investigation indicate that verotoxigenic Escherichia coli (VTEC) belonging to enteropathogenic and other serogroups including Escherichia coli O157:H7 or H- are important enteropathogens in infants and toddlers in Czechoslovakia. As to enteropathogenic serotypes, verotoxin (VT) production was proved most frequently in strains of serogroup O26, and also O111 and O128. Diseases caused by them were as a rule manifested by febrile watery diarrhoea with mucus in the stool. In two of five infants with Escherichia coli O26 :H11 with VT1 production in titres of greater than or equal to 1:512 (blood was present) in the stool and one suffered from marked abdominal pain. In one infant haemorrhagic colitis due to Escherichia coli O157:H- was found. Haemolytic uraemic syndrome associated with VTEC of serogroups O157, O26, O18, O5 and O1 with VT1 and/or VT2 was observed in six children including five who contracted the disease during an outbreak in a small town, and the source of infection was probably contaminated water. Five children recovered and one died; the postmortem examination revealed haemorrhagic colitis and necrosis of the renal cortex. Haemorrhagic colitis caused by Escherichia coli O157 in infants and toddlers differed from the course hitherto described in older subjects by fever and the presence of mucus in the stools.

Bacterial Toxins↗