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M Boguslawski

Publications and source records attributed to M Boguslawski.

6 recordsLinked to original sources
PubMed1975

Phosphopyridoxal complexes with histamine and histidine. (1) The kinetics of complex formation between pyridoxal 5'-phosphate and histamine.

Amines and amino acids are able to form cyclic compounds with pyridoxal 5'-phosphate (PLP). The quantitative relationship between histamine and PLP in the cyclization reaction were examined. It was found that one mole of amine reacts with one mole of coenzyme. The velocity of complex formation increased with an excess of either histamine or PLP, without any change in molar ratios. The formation of cyclic compound was confirmed by the use of isotopic method. The separation of cyclic compound from histamine, but not from PLP, was achieved by paper chromatography.

Chemical Phenomena↗
PubMed1975

Phosphopyridoxal complexes with histamine and histidine. (3) The influence of presumed complex on activity of rat intestinal histaminase.

Histamine in high concentration inhibits the rat intestinal histaminase (diamine oxidase E.C. 1.4.3.6.). The apparent Km is approximately 4.2 X 10(-5) M. This inhibition can be reversed by an addition of PLP. It was also found that excess of PLP inhibits enzyme activity. It is competitive inhibition. Histamine and other amines which were associated with enzyme inhibition form spectrophotometricaly demonstrable complex with PLP. The possible mechanism of the inhibitory action of PLP and complex with histamine and other amines on rat intestinal histaminase activity are discussed.

Amine Oxidase (Copper-Containing)↗