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M Boogaerts

Publications and source records attributed to M Boogaerts.

At least 91 records · Page 5Linked to original sources
PubMed1988

Evaluation of 111In labelled white blood cells by in vitro functional tests and electron microscopy. Comparison of three labelling methods.

We have studied the influence of granulocyte labelling with commercially available 111In-oxine, tropolone (trop) or home made 111In-Mercapto pyridine (Merc) prepared by the method of Thakur (1985) on the cell structure by electron microscopy and on the cell function by enzymatic tests, random migration, chemotaxis, phagocytosis and bactericidal activity. The granulocytes were labelled with 400 microCi 111In-oxine in saline or 111In-trop or Merc in plasma. The effect of the chelating agents with and without addition of the tracer was studied (n = 4) with varying concentrations: 5-10 micrograms/ml oxine, 10-160 micrograms/ml trop and 1-4 micrograms/ml Merc. Chemotaxis and random migration were not affected by 111In-trop and clearly suppressed by 111In-oxine and Merc; the other tests were normal. The cell structure was disturbed by Merc. The labelling efficiency was excellent with oxine (90%), acceptable with trop (30%-80%) and poor with Merc (10%-25%). Without 111In, chemotaxis and random migration were normal up to a concentration of 80 micrograms/ml trop, 8.5 micrograms/ml oxine and 1 microgram/ml Merc. With addition of 111In, chemotaxis and random migration were unaffected up to 80 micrograms/ml by trop and markedly suppressed by Merc and oxine. It is concluded that labelling with 111In-trop assures intact cells.

Granulocytes↗
PubMed1988

Cytogenetic studies in T-cell malignancies.

Clonal chromosome abnormalities in ten cases of T-cell lymphoproliferative disorders are reported. In addition, an extensive review of the literature on T-cell lymphomas shows the chromosomal sites most frequently involved in structural rearrangements.

Adolescent↗
PubMed1988

The karyotype in secondary hematologic disorders after treatment for Hodgkin's disease. A study of 19 patients.

In 19 cases of secondary hematologic disorders in patients previously treated for Hodgkin's disease, chromosome aberrations were analyzed in relation to the type of previous chemo- and/or radiotherapy, age of the patients, histopathologic features of the Hodgkin's disease at diagnosis, time interval between the treatment and the occurrence of the secondary disorder, and survival. The karyotype was of significant prognostic value when three cytogenetic groups were considered: patients with normal karyotypes; patients with aberrations of chromosome 7 as the sole anomaly; and patients with complex rearrangements and translocations. The last group showed the lowest rate of survival. Bone marrow transplantation was successful in two patients with a normal karyotype.

Adult↗
PubMed1988

The short arm of chromosome 6 is nonrandomly rearranged in secondary myelodysplastic syndromes.

Eight patients with a myelodysplastic syndrome showed a structural rearrangement of the short arm of chromosome #6 involving the distal segment 6p22----6pter. In four cases the myelodysplastic disorder appeared after treatment with chemo- and/or radiotherapy. Cytogenetically, the 6p anomaly was consistently associated with abnormalities of chromosome #5 and/or #7 in seven of eight cases. We believe we identified a new site on 6p that is nonrandomly involved in iatrogenically and possibly also environmentally induced malignant hematologic disorders.

Adult↗
PubMed1988

More familial leukaemia in ALL patients with both unexplained high transcortin levels and an HLA antigen Cw3.

In a group of 74 adult ALL patients 25 had a transcortin level greater than or equal to 3 SD above the normal mean, 15 expressed an HLA-Cw3 antigen and 11 combined both characteristics. In this subgroup of 11 ALL patients, five had familial leukaemia, i.e. at least one relative with acute leukaemia or lymphoma. On the other hand, only three examples of familial leukaemia were found in a series of 59 ALL patients with one or none of these two characteristics (relative risk: 15.6; P less than 0.001).

Adult↗
PubMed1987

Chromosome studies in patients with Philadelphia chromosome-positive chronic myeloid leukemia submitted to bone marrow transplantation--results of a European Cooperative Study.

The karyotypes of 74 patients with Ph-positive chronic myeloid leukemia submitted to bone marrow transplantation collected from nine institutions were studied serially before and after transplantation. In 13 cases sporadic Ph-positive metaphases were detected without signs of relapsing disease at various intervals after transplantation. These data indicate that the leukemic clone may not be completely suppressed by the conditioning treatment and that other biological mechanisms may be involved in destroying this clone or controlling its expansion.

Adolescent↗
PubMed1987

Chromosome studies in patients with acute nonlymphocytic or acute lymphocytic leukemia submitted to bone marrow transplantation--results of a European cooperative study.

The chromosomal data of 58 acute nonlymphocytic leukemia (ANLL) patients and of 32 acute lymphocytic leukemia (ALL) patients submitted to bone marrow transplantation and collected from nine institutions are reported. Chromosomal studies were available at diagnosis in 19 cases with ANLL: seven had a partially or completely abnormal pattern. Forty-one patients had a chromosome study before bone marrow transplantation and all had a normal pattern. Thirteen patients with ALL were studied at diagnosis: five had a partially or completely abnormal karyotype. Of 20 cases analyzed before bone marrow transplantation, only one has maintained the abnormal pattern of diagnosis in part of the cells. Karyotypes were available in nine ANLL patients relapsed after bone marrow transplantations. Two showed the same clonal abnormalities seen at diagnosis; in three other cases the leukemic clone of relapse carried an additional chromosome abnormality with respect to the pattern at diagnosis and four more cases presented at relapse complex abnormalities; two of them had a cytogenetically normal pattern at diagnosis. In four of ten relapsed ALL cases chromosomes analyses were available. A relapse in donor cells and a hyperdiploid pattern were observed in two cases, respectively, while a normal, recipient pattern was documented in the other two cases. Serial chromosome studies performed in acute leukemia patients after bone marrow transplantation may allow the detection of different chromosomal patterns of relapse. In those cases who relapsed with a cell clone cytogenetically different from the pattern at diagnosis, a direct role played by the conditioning treatment in the pathogenesis of the relapsing disease may be hypothesized.

Acute Disease↗
PubMed1986

An identical translocation between chromosome 1 and 15 in two patients with myelodysplastic syndromes.

An identical translocation between the long arm of chromosome no. 1 and the short arm of chromosome no. 15 was found in two unrelated patients with refractory anaemia type I, according to the FAB classification of myelodysplastic syndromes. In the first patient the typical translocation was associated with anomalies commonly found in preleukaemic states, i.e. a 5q- and a 20q- chromosome. Furthermore, in both patients the long arm of chromosome no. 1 was trisomic. Cytogenetic follow-up in the second patient demonstrated a proliferative advantage of the cells bearing a t(1;15) translocation over the cells with trisomy 8 as well as over normal cells. This karyotypic evolution, however, was not accompanied by a transformation of the haematological disorder into acute leukaemia.

Anemia, Refractory↗
PubMed1985

Defective host defence mechanisms in a family with hypocalciuric hypercalcaemia and coexisting interstitial lung disease.

An extensive in vitro investigation of the host defence system was performed in 11 sibs of a large kindred with unexplained combination of familial hypocalciuric hypercalcaemia (FHH), interstitial lung disease and increased susceptibility to respiratory infections. The impairment of host defence mechanism was most likely related to granulocyte dysfunction. A severe myeloperoxidase deficiency was the most consistent granulocyte defect (P less than 0.001) and it was associated with a relatively diminished chemiluminescence (P less than 0.001). Moreover, a significantly diminished antistaphylococcal phagocytic (P less than 0.001) and killing (P less than 0.001) activity was found which in the absence of any opsonizing defect implicates an intrinsic granulocyte dysfunction. We found no abnormalities in number of B and T lymphocytes nor in the balance between helper and suppressor cells determined with monoclonal antibodies. Despite the recurrent infections no elevations of the immunoglobulin subclasses were found. The relationship between the inherited FHH, interstitial lung disease and susceptibility to respiratory infections remains obscure. It is, however, clear that impairment of the host defence might contribute to a decreased life expectancy in this family.

Adult↗