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Biomedical subjects

M Boone

Publications and source records attributed to M Boone.

13 recordsLinked to original sources
PubMed2003

Two cases of cross-sensitivity in subjects allergic to paraphenylenediamine following ingestion of Polaronil.

We report the cases of 2 women presenting allergy to paraphenylenediamine (PPD). Both patients had a history of eczema that worsened following the ingestion of the antihistamine Polaronil (dexamethasone/dexchlorpheniramine). This clinical presentation could be explained by cross-sensitivity to sulfanilic acid (4-aminobenzene sulfonic acid), a metabolite of sunset yellow (FD&C No. 6). Sunset yellow is an azo dye present in this tablet. Indeed, PPD-allergic subjects may suffer from cross-sensitivity to related compounds, especially to those that can be ingested such as azo dyes. Such compounds are used in some instances by the food and pharmaceutical industries, but their presence is often undisclosed.

Adult↗
PubMed2000

Viral genome organizer: a system for analyzing complete viral genomes.

The viral genome organizer (VGO) is designed to simplify the characterization and annotation of complete viral genomes (particularly those of large poxviruses) and to help researchers discover new genes and detect gene fragmentation. VGO is based on Genotator [Harris, N.L., 1997. Genome Res. 7, 754-762], an annotation workbench designed for the analysis of eukaryotic genomic sequences. VGO automates a number of database search routines (FASTA, BLASTP, PSI-BLAST and TBLASTN), processes the results through a multiple-alignment viewer (MView; [Brown, N.P., Leroy, C., Sander, C. , 1998. Bioinformatics 14, 380-381]) and serves to manage the hundreds of DNA, protein and database search results files that must be organized when dealing with large complete poxviral genomes. It also directs the generation a self-dotplot of the genome by Dotter [Sonnhammer, E.L.L., Durbin, R., 1995. A dot-matrix program with dynamic threshold control suited for genomic DNA and protein sequence analysis. Gene 167: GC1-10. http://www.sanger.ac. uk/Software/Dotter/] to uncover repeated genes and sequences and provides Internet links to programs for generation of restriction maps and analysis of potential PCR primers. The user-friendly graphical interface displays DNA and protein sequences, links to search results, ORFs, stop-start codons, restriction sites and flags of database searches. Currently, VGO and associated programs run in an X-windows environment on commonly available UNIX machines.

Databases, Factual↗
PubMed2000

Adhesion molecule profiles in atopic dermatitis vs. allergic contact dermatitis: pharmacological modulation by cetirizine.

BACKGROUND: Experimental data suggest that there is an imbalance between Th1 and Th2 cells in atopic dermatitis (AD) skin compared to allergic contact dermatitis (ACD). This imbalance (Th2 and Th1 predominance, respectively) implies the production of different cytokines in these two conditions leading to different expression of adhesion molecules on skin endothelial cells. OBJECTIVE: The expression of VCAM-1 (IL-4/Th2-dependent) and ICAM-1 (INF-gamma/IL-1) on dermal vessels was compared in six patients with AD and six patients with ACD. The effect of cetirizine, a highly selective H1-receptor antagonist on the expressions was studied. METHODS: Six patients with AD were challenged with Dermatophagoides pteronyssimus (DPT patch tests applied to clinically normal skin) and six patients with ACD challenged in the same way with allergens of the European standard series. Skin biopsies at challenged sites were performed before and 6, 24 and 48 h after challenge. The experiment was carried out under double-blind cross-over conditions during a 4-day treatment with a placebo and cetirizine. RESULTS: In AD patients, the scores for both VCAM-1 and ICAM-1 were high before and after challenge. In ACD patients, the ICAM-1 score was high at each experimental time, but the VCAM-1 score, which was significantly lower before challenge, increased at 6, 24 and 48 h after challenge. The administration of cetirizine significantly reduced the VCAM-1 expression in AD patients at each experimental time. CONCLUSION: It is concluded that the increased VCAM-1 expression in AD patients compared to ACD may reflect greater IL-4 and/or IL-13 production in situ. The study also confirms the existence of a modulating effect of cetirizine in vivo on adhesion molecule expression.

Adult↗
PubMed2000

BLAST Search Updater: a notification system for new database matches.

UNLABELLED: BLAST Search Updater (BSU) can help molecular biologists perform large numbers of BLAST searches routinely and screen the results for novel matches. The search result files (HTML format) and delta files (novel matches) are WWW viewable and BSU also emails users alignments of new matches. AVAILABILITY: BSU runs on UNIX machines and is freely available to academic users at http://athena.bioc.uvic.ca/genomes/. Additional figures and description are also available. CONTACT: cupton@uvic.ca

Computational Biology↗
PubMed1998

Digital image analysis of the effect of topically applied linoleic acid on acne microcomedones.

A major pathogenic factor of acne is the disturbed keratinization of the follicular infundibulum. It has been hypothesized that a relative decrease in linoleic acid in the sebum could be responsible, in part, for this. The aim of the present study was objectively to evaluate the effects of topically applied linoleic acid on the size of microcomedones in patients with mild acne. The design was a double-blind placebo-controlled randomized cross-over study. Evaluations were performed by digital image analysis of cyanoacrylate follicular biopsies. There was a significant effect of topically applied linoleic acid on the size of follicular casts and microcomedones, an almost 25% reduction in their overall size being achieved over a 1-month treatment period. In contrast, no change was found at placebo-treated sites. It is concluded that topical linoleic acid might play a role as a comedolytic agent in acne-prone patients.

Acne Vulgaris↗
PubMed1995

Participation in transvaginal ovarian cancer screening: compliance, correlation factors, and costs.

The objective was to identify factors influencing participation in screening for ovarian cancer using transvaginal sonography in the free experimental program at the University of Kentucky over its 6+ year history. Database records for screenings, performed from 1987 to June 1994, were utilized. Computer sorts, 1990 census information as predictors, and stepwise multiple regression analysis were employed. Participation in the model ovarian screening program took 3-4 years to approach > 300 screens/month, with repeat screenings exceeding new subject participation in this time period. A number of participants traveled > 200 miles for screening on both initial and repeat encounters. Analysis of distance to the screening site, median family income, county physician population, and education levels indicated that distance and then education correlated best with participation. Unit screening cost shrank from $45 to under $25 when maximal participation was achieved. Distance and education correlated with participation. Expenses compare favorably with diagnostic procedures for other diseases.

Adult↗
PubMed1995

Eosinophilic pustular folliculitis in three atopic children with hypersensitivity to Dermatophagoides pteronyssinus.

Three children will be described who present recurrent episodes of pruritic papulopustular follicular lesions on the face, the extremities and the trunk. The episodes lasted for 1-3 months with intermittent remission. Each flare was accompanied by hypereosinophilia and an increased total IgE titer. RAST and prick tests were positive for Dermatophagoides pteronyssinus (DPT). Laboratory tests disclosed no infectious or parasitic etiology. Histological examination showed eosinophilic pustular folliculitis (EPF) in each of the 3 cases. The lesions responded well to topical corticosteroids. The aim of this article is to underline the importance of hypersensitivity reactions (in these particular cases to DPT) in the pathogenesis of EPF.

Adolescent↗
PubMed1980

Effect of a denture adhesive on complete denture dislodgement during mastication.

A method of evaluating the denture stabilizing effects of a denture adhesive by means of counting denture dislodgements during the eating of a standardized portion of a test food is described. A reduction in the number of such dislodgements after application of a denture adhesive, as compared to eating the same food without adhesive applied to the denture, was used as a measure of adhesive effectiveness. Subject responses to a number of denture-performance satisfaction parameters were also obtained. With a test adhesive, significant reductions in dislodgement were found using steak, taffy-apple, a hard-roll sandwich, and celery as test foods among 16 subjects with dentures that fit well. Analysis of subjective evaluations indicated that the test adhesive was perceived as having effected: (1) improved chewing ability, (2) reduced denture wobble, (3) improved confidence, (4) increased comfort, and (5) reduced amount of food particles collecting under the denture, all to a significant degree (p < .01). It is suggested that additional data from controlled clinical studies are needed to allow proper assessment of the usefulness and possible abuse effects of denture adhesive products. This method, and the resulting data, are offered as a stimulus to the end.

Adhesives↗
PubMed1975

Clinical, radiographic, and histological study of endodontically treated retained roots to preserve alveolar bone.

Endodontically treated, submerged roots in two Macaca speciosa monkeys were studied clinically, radiographically, and histologically. The roots were successfully covered by soft tissue, except in two areas. In several sites, radiographic evidence of bone formation was observed; this was confirmed by histologic examination. Bone formation coronal to the submerged roots was not a predictable occurrence. Even though epithelium and inflammation commonly occurred over the amputation sites, their presence did not seem to affect bone formation.

Alveolar Bone Loss↗
PubMedDate not supplied

Strength through sharing: interdisciplinary teamwork in providing health and social services to northern native communities.

The delivery of health and social services in Canada's northern First Nations is undermined by the fact that professionals from outside and para-professionals from the communities often fail to respect one another's capabilities or to understand one another's roles and, consequently, do not work well together. This paper explores the personal, professional, and situational causes, using examples of mental health care in the Sioux Lookout Zone of northwestern Ontario. Arguing that an interdisciplinary team approach is the ideal and, perhaps, the only real way in which essential services can be delivered, the authors suggest ways to achieve more effective collaboration.

Adolescent↗