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Biomedical subjects

M Botta

Publications and source records attributed to M Botta.

At least 91 records · Page 5Linked to original sources
PubMed1987

Two CEA cross-reacting antigens: differences in absorbing the same anti-CEA serum.

A factor that cross reacts with the carcinoembryonic antigen (CEA), which we call P factor, was isolated from normal human plasma. To demonstrate the difference between this P factor and the nonspecific cross-reacting antigen (NCA), the same anti-CEA serum was absorbed in an identical manner with both the antigens. Absorption was checked by immunohistochemistry by the beta-galactosidase procedure on sections of colonic adenocarcinoma and normal colonic mucosa. The unabsorbed antiserum recognizes both tissues; after absorption with NCA the staining becomes paler on both tissues, but maintains color on the normal colonic mucosa and granulocytes. Only absorption with the P factor will give an unstained field of normal colonic mucosa, thus revealing the tumor structure. Data obtained by us suggest that the NCA (tissue extract) is an antigen that is not suitable to the absorption of anti-CEA serum for immunocytochemistry techniques, whereas the P factor (plasma extract) appears to be utilizable with good results.

Absorption↗
PubMed1986

Peptide sequencing by using a combination of partial acid hydrolysis and fast-atom-bombardment mass spectrometry.

To overcome the limit of the intensity of ions carrying sequence information in structural determinations of peptides by fast-atom-bombardment m.s., we have developed a method that consists in taking spectra of the peptide acid hydrolysates at different hydrolysis times. Peaks correspond to the oligomers arising from the peptide partial hydrolysis. The sequence can then be identified from the structurally overlapping fragments.

Amino Acid Sequence↗
PubMed1985

Anti-carcinoembryonic antigen sera acquiring higher tumor specificity by absorption with a plasma factor.

A factor reacting with antisera against carcinoembryonic antigen (CEA) was isolated from healthy donors' plasma. Ten different antiCEA sera were absorbed with this plasma factor (P-factor): the antiCEA activity was mostly, or completely, removed in 8 sera. Only 2 of these absorbed sera (here defined as type A) still presented a high CEA binding activity by radioimmunoassay and, on tissue sections, selectively stained most of the colonic adenocarcinomas and areas of severe dysplasia in neoplastic adenomas. Contrary to the behaviour of the antisera against the P-factor, of the unabsorbed antiCEA sera, of a commercially available antiserum and of a monoclonal antibody, the P-absorbed type A antiCEA sera appeared unreactive with normal colonic mucosa and granulocytes. Absorption with the P-factor allows us to obtain antiCEA sera with higher tumor specificity.

Adenocarcinoma↗
PubMed1985

[Myocardial protection in valvular surgery by retroperfusion of the coronary sinus with cardioplegic solution in valve surgery. Immediate results and technical considerations].

Retrograde coronary sinus perfusion as a means of delivering cardioplegia was evaluated in 20 patients undergoing cardiac valve surgery. Other 10 patients, undergoing similar operation with antegrade coronary cardioplegic perfusion served as a control. Results showed no significant differences between the two groups of patients in terms of: myocardial cooling; pre-ischemic and post-perfusion myocardial lactate extraction; post-operative clinical findings (myocardial infarction occurrence, need for inotropic support, mortality). We conclude that retroperfusion through the coronary sinus is a safe and effective alternative of cardioplegic delivery. Since it simplifies the operative procedure (it allows the cardioplegia delivery at any desired time of the operation, without discontinuing the procedure), we recommended its use during valvular cardiac operations, especially on aortic valve replacement.

Heart Arrest, Induced↗
PubMed1984

[Obstruction of the superior vena cava caused by sclerosing mediastinitis. Surgical treatment by bypass with a composite spiral venous graft].

A 68-year-old woman with obstruction of the superior vena cava due to sclerosing mediastinitis was successfully operated on. A composite spiral vein graft was interposed between the left innominate vein and the right atrium to bypass the occluded superior vena cava. The graft was made using the patient's own saphenous vein, which was divided longitudinally and sutured around a cannula in a spiral fashion. Indications to surgical intervention in patients with superior vena cava syndrome are discussed.

Aged↗
PubMed1982

BCA (breast cancer antigens): different purification extraction methods.

Various types of extraction were tested to increase the immunological yield of BCA, a CEA-like primary breast cancer associated carcinoma antigen. To allow a comparison, the different extraction techniques were applied to only one breast tumour. The comparison of the various systems was based on two parameters: protein yield and immunological activity, assayed in a RIA 125I CEA-anti CEA system. The following extraction methods were described and compared in this paper: 3M KCl; 1N HClO4; neutral pH extraction (PBS) in the absence and presence of various detergents (anionic, neutral and cationic), basic pH extraction (1N NaOH) and acid pH extraction (1.5M acetic acid) in the presence of urea and various detergents. The more significant systems were applied also to the extraction of CEA, from colonic adenocarcinoma liver metastases. The best results for both the antigens studied were obtained by using neutral detergents (1% NP 40) at neutral pH.

Antigens, Neoplasm↗
PubMed1981

[Post-infarction aneurysm of the left ventricle. Pre-operative parameters conditioning the surgical risk and remote results].

83 patients who underwent resection of postinfarction aneurysms involving the anterior wall of the left ventricle were studied to identify possible determinants of operative outcome, long-term survival and long-term improvement. The indications for aneurysmectomy (either isolated or combined with direct revascularization) were congestive heart failure, angina or life-threatening arrhythmias; six patients were asymptomatic at the time of surgery. Operative mortality rate was 14.4% (11.5% for patients operated more than 60 days following myocardial infarction). The over-all survival rate, at a mean follow-up of 31.5 months (range 2 to 82 months), was 74.39%; 80.88% of patient discharged from hospital, exhibited clinical improvement. Mean values of ejection fraction and excess ejection fraction were significantly higher in the group of operative survivors (31.28 +/- 8.26% and 0.17 +/- 0.08 respectively) than those of surgical deaths (20.25 +/- 8.37% and 0.08 +/- 0.06) (p less than 0.005); the coronary score was significantly lower in the group of survivors (6.81 +/- 2.35 vs 8.33 +/- 2.29) (p less than 0.025). Presence of arrhythmias increased operative mortality (33.3% vs 11.2%, p = 0.05), as did a low cardiac output, impaired contraction of the postero-lateral wall and mitral regurgitation. Long-term survival and improvement were not related to anyone of preoperative parameters taken into consideration: however, a higher percentage of clinical improvement was observed when myocardial revascularization was associated to aneurysmectomy (85% vs. 67.7%).

Adult↗
PubMed1979

[Ebstein's malformation. Report of 9 cases surgically treated (author's transl)].

Nine patients with Ebstein's malformation have undergone surgical repair since 1971. Their ages ranged from 10 to 49 years. Two patients were in functional class II and six in III. Dysrhythmias were indicators for operation in the remaining patient. All patients underwent prosthetic valve replacement. Other procedures included atrial septal defect closure in eight and plication of the atrialized ventricular segment in four cases. Three patients died during the postoperative period. Three patients died during the postoperative period. Functional classification improved in five of the six operative survivors. The multiple aspects of the clinical features, the different surgical management and the variability of results are explained by the wide range of morphologic and functional aspects of the malformation.

Adolescent↗
PubMed1976

[Rupture of interventricular septum secondary to blunt chest trauma. Report of a case surgically treated with success (author's transl)].

A case of interventricular septal defect secondary to blunt chest trauma is reported. It was characterized by a disconnection of the interventricular muscolar septal in the anterosuperior part of the heart wall. The diagnosis, suspected by clinical and instrumental parameters, was definitely confirmedy by hemodynamic and contrastographic examination. Repair surgery of the defect with a dacron patch was performed, using extracorporea normothermic circulation seven months after the accident. The patient was examined three months and twelve months after the operation; the clinical examinations did not reveal any cardiac murmur and the patient's health was satisfactory. The incidence, mechanism of rupture of interventricular septum and the main surgical and clinical aspects of this type of pathology are discussed.

Adult↗
PubMedDate not supplied

Variable selection and specification of robust QSAR models from multicollinear data: arylpiperazinyl derivatives with affinity and selectivity for alpha2-adrenoceptors.

Two QSAR models have been identified that predict the affinity and selectivity of arylpiperazinyl derivatives for alpha1 and alpha2 adrenoceptors (ARs). The models have been specified and validated using 108 compounds whose structures and inhibition constants (Ki) are available in the literature [Barbaro et al., J. Med. Chem., 44 (2001) 2118; Betti et al., J. Med. Chem., 45 (2002) 3603; Barbaro et al., Bioorg. Med. Chem., 10 (2002) 361; Betti et al., J. Med. Chem., 46 (2003) 3555]. One hundred and forty-seven predictors have been calculated using the Cerius 2 software available from Accelrys. This set of variables exhibited redundancy and severe multicollinearity, which had to be identified and removed as appropriate in order to obtain robust regression models free of inflated errors for the beta estimates - so-called bouncing betas. Those predictors that contained information relevant to the alpha2 response were identified on the basis of their pairwise linear correlations with affinity (-log Ki) for alpha2 adrenoceptors; the remaining variables were discarded. Subsequent variable selection made use of Factor Analysis (FA) and Unsupervised Variable Selection (UzFS). The data was divided into test and training sets using cluster analysis. These two sets were characterised by similar and consistent distributions of compounds in a high dimensional, but relevant predictor space. Multiple regression was then used to determine a subset of predictors from which to determine QSAR models for affinity to alpha2-ARs. Two multivariate procedures, Continuum Regression (the Portsmouth formulation) and Canonical Correlation Analysis (CCA), have been used to specify models for affinity and selectivity, respectively. Reasonable predictions were obtained using these in silico screening tools.

Models, Molecular↗