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Biomedical subjects

M Bouteille

Publications and source records attributed to M Bouteille.

17 recordsLinked to original sources
PubMed1979

Mechanisms of transcription in nucleoli of amphibian oocytes as visualized by high-resolution autoradiography.

In oocytes of Pleurodeles waltlii, the method of Miller and Beatty has been combined with a method of high-resolution autoradiography especially suitable for the study of isolated molecules. In vitro labeling of RNA by tritiated precursors was carried out with increasing incubation times (1, 4, 15, 24, 48, and 72 h). Silver grains were present over ribonucleoprotein fibrils in amounts sufficient for quantitative analysis of nucleolar DNA transcription. Statistical analysis of the data revealed that: (a) The units of any one nucleolus exhibited a large degree of heterogeneity in their number of grains. (b) There was a parallelism between the increasing grain number and the ribonucleoprotein-fibril lengthening as observed along the transcription unit.

Amphibians↗
PubMed1979

Localization of a gene: the nucleolar organizer.

Nucleolus differentiates around the nucleolus organizer regions of the chromosomes or NORs. In the interphasic nucleoli the fibrillar centers are now considered as the NORs. The purpose of this editorial is to review the experimental data which allowed such identification. Our current concepts regarding this point result from three lines of evidence: 1) specific localization during nucleologenesis, 2) in situ hybridization with labeled ribosomal RNA or DNA, and 3) specific staining with silver.

Cell Cycle↗
PubMed1978

Electron microscope fluoro-autoradiography: improvement of efficiency.

The fluorographic process for enhancement of the autoradiographic detection of beta-ray emitted by tritium has been successfully adapted to electron microscope autoradiography. An original scintillator plastic film helped to increase the detection yield of electrons up to 80% without alteration of the morphological aspect of the autoradiograms, and without increasing background fog or causing apparent loss of resolution.

Animals↗
PubMed1978

[Ultrastructural identification of the nucleolus organizer by the silver staining technic].

The nucleolus organizer regions can be selectively stained in metaphase chromosome preparations by the Goodpasture and Bloom's technique which was adapted to electron microscopy analysis of cells during interphase. Using this technique, a selective accumulation of silver grains was observed over nucleolus light areas. This selective accumulation allows the identification of the interphase fibrillar centers as the nucleolus organizer regions. Ultrastructural relationships between fibrillar centers and dense fibrillar component are discussed.

Cell Nucleolus↗
PubMed1978

[Application of immunoelectron microscopy to the detection of viruses in a water medium].

Adenoviruses were immersed in demineralyzed and deionized water for 5 days. The water was subsequently analyzed by Immuno-Electron-Microscopy for detection of viral particles. An attempt of quantation was also made on control, untreated particles. Viruses dispersed in water can be detected by the technique employed but quantitation is limited by the heterogeneous dispersion of the particles on the grids.

Adenoviridae↗
PubMed1977

Infection of cultivated CNS tissue with herpes virus, HSVII. A reappraisal.

Organized cultures of newborn rat and hamster cerebellum were infected with herpes virus type II, after 7 and 14 days "in vitro". 48 h after the infection, electron microscopic examination of the cultures showed that astrocytes contained numerous intranuclear and intracytoplasmic viral particles, while neurons remained apparently intact. The specificity of the infection for a given cell type is discussed.

Animals↗