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M Bruining

Publications and source records attributed to M Bruining.

6 recordsLinked to original sources
PubMed2004

The use of polymer-based electrospun nanofibers containing amorphous drug dispersions for the delivery of poorly water-soluble pharmaceuticals.

Electrostatic spinning was applied to the preparation of drug-laden nanofiber for potential use in oral and topical drug delivery. While this technique is in its infancy with regard to pharmaceutical applications, a number of recent publications suggest that it may be of high value in the formulation of poorly water-soluble drugs by combining nanotechnology and solid solution/dispersion methodologies. The purpose of this article is to describe some of these recently published applications. For immediate release oral application, a water-soluble cellulose polymer was selected (i.e., hydroxypropylmethylcellulose, HPMC) while for topical application, a nonbiodegradable, water-insoluble polymer was investigated (i.e., a segmented polyurethane, SPU). Solutions of the polymer and the drugs in appropriate solvents could be spun across various potentials (16-24 kV) generating nanofibers with diameters ranging from 300 to 2000 nm. Dissolution studies found that the non-woven fabrics derived from HPMC and containing itraconazole dissolved over a time course of minutes to hours depending on the formulation used as well as the drug/polymer ratios. Drug release from the SPU samples was dependent on the incorporated drug as well as nanostructure obtained.

Antifungal Agents↗
PubMed1995

Passage rate and total clearance rate from the rumen of cows fed on grass silages differing in cell-wall content.

Four non-lactating, rumen-fistulated cows were fed ad lib. on two grass silages (first cut (FC) and second cut (SC) harvested at different growth stages, resulting in different crude-protein (CP) and neutral-detergent-fibre (NDF) contents (FC, 152g CP/kg, 515g NDF/kg and SC, 210g CP/kg, 442g NDF/kg). Voluntary intake and rumen contents, total as well as organic matter were higher for silage FC. Fractional passage rate from the rumen, calculated from the logarithmic decline in Cr-NDF rumen pool, was higher for silage FC (0.0395/h and 0.0446/h for silages SC and FC respectively). When fractional passage rates from the rumen were calculated by dividing the intake of indigestible organic matter by the mean rumen pool of this fraction, the same differences between silages were found, although the actual levels were much lower (0.0258/h and 0.0300/h for silages SC and FC respectively). The results from the present experiment suggest that disappearance rate from the rumen of particles with a size between 1.25 and 0.071 mm is the rate-limiting step in the control of rumen fill.

Animals↗
PubMed1993

Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume.

The effect of rumen liquid volume, pH and concentration of volatile fatty acids (VFA) on the rates of absorption of acetic, propionic and butyric acids from the rumen was examined in lactating dairy cows. Experimental solutions introduced into the emptied, washed rumen comprised two different volumes (10 or 30 l), four levels of pH (4.5, 5.4, 6.3, 7.2) and three levels of individual VFA concentrations (20, 50 or 100 mM-acetic, propionic or butyric acid). All solutions contained a total of 170 mM-VFA and an osmotic value of 400 mOsmol/l. Absorption rates were calculated from the disappearance of VFA from the rumen corrected for passage with liquid phase to the omasum. An increase in initial fluid pH caused a reduction in fractional absorption rates of propionic and butyric acids. Increasing the initial pH from 4.5 to 7.2 reduced fractional absorption rates of acetic, propionic and butyric acids from 0.35, 0.67 and 0.85 to 0.21, 0.35 and 0.28/h respectively. The fractional absorption rates of all VFA were reduced (P < 0.05) by an increase in initial rumen volume. The fractional absorption rate of acetic acid was lower (P < 0.05) at an initial concentration of 20 mM than of 50 mM. The fractional absorption rate of propionic acid tended (P < 0.10) to decrease as the level of concentration increased while fractional absorption rate of butyric acid was not affected by butyric acid concentration. These results indicate that relative concentrations of VFA in rumen fluid might not represent relative production rates and that attempts to estimate individual VFA production from substrate digestion must take account of pH and VFA concentration.

Acetates↗