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Biomedical subjects

M Bruni

Publications and source records attributed to M Bruni.

18 recordsLinked to original sources

Evaluation of new computerized method for recording 7-day food intake in IDDM patients.

OBJECTIVE: To evaluate a new computerized method for recording 7-day food intake. RESEARCH DESIGN AND METHODS: Randomized crossover trial was conducted with patients recording the amount and type of every food and drink consumed during a week by either a computerized device (Food-meter) or recording the data in a diary. Each method was applied twice. Twenty-one insulin-dependent diabetic patients (mean +/- SD age 25 +/- 9 yr) were studied. RESULTS: The two methods showed very good agreement in the evaluation of the patients' diets (1792 +/- 408 vs. 1764 +/- 436 kcal/day, 84 +/- 19 vs. 82 +/- 21 g/day protein, 68 +/- 22 vs. 67 +/- 23 g/day fat, 210 +/- 60 vs. 207 +/- 58 g/day carbohydrate with the conventional and computerized methods, respectively). The variability between the methods and the variability within each method were of similar magnitude. CONCLUSIONS: The Food-meter represents a useful tool for computerizing the 7-day food record. The method is easy, reliable, and time saving. Moreover, it minimizes the risk of transcriptional errors.

Adult

Asymmetrical distribution of beta-endorphin in cerebral hemispheres of suicides: preliminary data.

The assessment of beta-endorphin levels in several symmetrical brain regions of seven suicides and seven sudden death controls reveals a decreased concentration in the left temporal cortex, the left frontal cortex, and the left caudate nucleus of suicides compared to controls. Moreover, the comparisons of beta-endorphin concentrations in the symmetrical brain regions reveals an asymmetrical concentration in suicides (left less than right) in frontal cortex and caudate nucleus. These data confirm previous reports of abnormalities of neurochemical pathways in the brains of suicides and suggest that suicidal behavior might be related to the lateralized mechanisms of mood control.

Adult

A follow-up of the protein pattern after operations in patients treated with an oral enzyme.

The sera of 32 patients have been studied in order to assess the behaviour of the main proteins, which could be identified with a two-dimensional electrophoresis, after operation. 20 of them were given an oral enzyme preparation of trypsin/chymotrypsin (Chymoral 100/F); the others were given no therapy. The analysis was performed comparing the relative concentration of 17 proteins in the pre-operative phase and the fifth day after the operation. Some variations known already and induced by this therapy (like the increase of alpha-1-antitrypsin), have been confirmed, but it has also been demonstrated, in the patients treated with Chymoral, a clear reduction of the constant loss of albumin and pre-albumin, which occurs after an operation.

Blood Protein Electrophoresis

Changes in the lung alveolar proteins in some experimental conditions.

The Authors have directed their research to the alveolar lining (the surfactant) proteins and they have particularly studied its alterations in shock pathological conditions. Some rats have been subjected to haemorrhagic and toxic shock, by Escherichia Coli's lipopolysaccharide; then lung washing has been performed and on extracted liquid proteins have been studied by two-dimensional immunoelectrophoresis (TDI). The obtained data have been encouraging and very useful for studies process.

Animals

The proteins of the lung alveolar lining.

The protein composition of the lung washing has been analysed by immunochemical methods: it has been possible to demonstrate the presence of many proteins, mainly albumin, immunoglobulins and beta-globulins, some of them seem laying in the alveolar lining and having the function of stabilizing the surfactant system.

Animals

[Ovarian thyroid].

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Adolescent