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Biomedical subjects

M Brunner

Publications and source records attributed to M Brunner.

33 records · Page 2Linked to original sources
PubMed1987

Enzyme immunoassay of H-Y antigen: experimental and clinical applications.

A newly developed enzyme-linked immunosorbent assay (ELISA) was applied for studying H-Y antigen in buffaloes, cattle, horses and humans. A monoclonal H-Y antibody was absorbed with cells from males or females and was then tested against fluid samples known to contain soluble H-Y antigen. In this system, positive absorption manifested itself by a fall in optical density relative to the optical density scored using unabsorbed antibody; this finding signified the presence of H-Y in the absorbing cells. In each of the four species, the fall in optical density was pronounced after absorption with male cells, but some decrease was also evident after absorption with female cells, indicating a degree of nonspecific attachment of the antibody.

Animals↗
PubMed1986

[Delayed development of hemispheric dominance accompanied by a rare form of retarded language development].

A severe and unusual form of retarded speech development in an eight-year old boy is analyzed as "right-hemispheric speech" against the background of neuropsychological theories on the specialisation of the cerebral hemispheres and considered in connection with the development of hemispheric dominance. A plan for therapy and teacher counselling is derived from this analysis. The influence of this retarded speech development on the acquisition of reading and writing is also touched upon.

Attention↗
PubMed1981

Folate deaminase and cyclic AMP phosphodiesterase in Dictyostelium discoideum: their regulation by extracellular cyclic AMP and folic acid.

Cyclic AMP and folic acid act as chemotactic factors in Dictyostelium discoideum. Both agents, when applied extracellularly, also control cell development from the growth stage to the acquisition of aggregation competence. Cyclic AMP phosphodiesterase and folate deaminase are extracellular enzymes whose activity is regulated during early differentiation of D. discoideum cells. The two enzymes help control the extracellular levels of cyclic AMP and folic acid. The substrates cyclic AMP and folic acid each increase the extracellular activity of folate deaminase as well as phosphodiesterase. The specificity of extracellular phosphodiesterase regulation by cyclic AMP indicates that the effect is mediated by specific cyclic AMP receptors rather than the catalytic site of cell surface phosphodiesterase. To some extent cyclic AMP and folic acid are interchangeable with respect to regulating differentiation and enhancing enzymatic inactivation of intercellular signals. Thus the two extracellular signals may share a common cellular pathway of signal transduction. The regulation of folate deaminase and phosphodiesterase by folic acid does not always parallel the folic acid effects on development. Pulses of folic acid stimulate development of aggregation competence, whereas a continuous flux inhibits. In contrast, either continuous flux or pulses of folic acid increase the deaminase and phosphodiesterase activities.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1975

[A multidisciplinary study of infantile Steinert's syndrome].

The authors studied from several points of view 10 patients suffering from a neo-natal form of Steinert's disease. They analyse the variety of the family histories, of dysembryoplastic symptoms, of neo-natal difficulties and the subsequent impairment of mental development. They could not find any correlation between this latter evolution and the severity of the neurological damage, judged by the mentioned symptoms. They conclude that the mental retardation is proportional to the low quality of the early socio-emotional environment and believe that this hypothesis is confirmed by the fact that maxillo-facial malformations are mocise speech and mimicry in their early childhood.

Female↗
PubMed1972

Processing of adenovirus RNA before release from isolated nuclei.

Nuclei isolated from cultured human cells (KB) infected with adenovirus type-2 were used to study events occurring during the adenosine triphosphate-dependent release of viral RNA. When cells were labeled with [(3)H]uridine for 50 min beginning 18 hr after infection, most nuclear viral messenger RNA was in the form of high molecular weight precursors with sedimentation coefficients greater than 28 S. When nuclei were incubated in the presence of ATP and an ATP-generating system, labeled RNA the size of viral mRNA (10-29 S) was released. Cleavage of viral RNA precursor molecules of high molecular weight occurred during incubation of nuclei. The change in size of viral RNA did not require exogenous ATP and occurred before release of RNA from nuclei. This RNA cleavage seems comparable to processing of mRNA in vivo, for discrete viral species were produced. Cleavage was completed after 10 min of incubation but was not the rate-limiting step in the release reaction, which required about 20 min for completion.

Adenosine Triphosphate↗
PubMed1969

Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens.

Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens V-1 is temperature-sensitive. At 25 C and 30 C, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 C, although bacterial growth was not inhibited, phage release was suppressed. Phage synthesis was induced by heat shock, 42 C for 30 min, ultraviolet irradiation, and mitomycin C. Induction by ultraviolet light was unusual-an immediate rise in phage titer followed irradiation. A large increase occurred after a 90-min latent period. The lysogenic strain was cured of the phage by incubation at 37 C, and the cured strain produced plant tumors.

Bacteriolysis↗
PubMedDate not supplied

VIPER: a general-purpose digital image-processing system applied to video microscopy.

This paper describes VIPER, the video image-processing system Erlangen. It consists of a general purpose microcomputer, commercially available image-processing hardware modules connected directly to the computer, video input/output-modules such as a TV camera, video recorders and monitors, and a software package. The modular structure and the capabilities of this system are explained. The software is user-friendly, menu-driven and performs image acquisition, transfers, greyscale processing, arithmetics, logical operations, filtering display, colour assignment, graphics, and a couple of management functions. More than 100 image-processing functions are implemented. They are available either by typing a key or by a simple call to the function-subroutine library in application programs. Examples are supplied in the area of biomedical research, e.g. in in-vivo microscopy.

Animals↗