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Biomedical subjects

M Buc

Publications and source records attributed to M Buc.

At least 73 records · Page 4Linked to original sources
PubMed1988

Genetic determination of phagocytic activity of polymorphonuclear leucocytes.

Sixty-three persons with the known HLA-A, -B, -C, -DR and -Dw antigens were investigated for phagocytic ability, candidacidal activity, respiratory burst values, and the levels of circulating immune complexes, C2, C4, IgG, IgM, and IgA. The investigated persons were divided into the HLA-DR2/Dw2, the HLA-DR3/Dw3 and a control group, members of which possessed neither antigen in question. The phagocytic activity of polymorphonuclear leucocytes ingesting dead cells of C. albicans and the candidacidal activity were found to be significantly lower (P less than 0.02) in the HLA-DR3/Dw3 compared to the control group. The respiratory burst values did not considerably differ in all three groups under study. The levels of circulating immune complexes were higher in both the HLA-DR2/Dw2 and HLA-DR3/Dw3 in comparison with the control group.

Antigen-Antibody Complex↗
PubMed1987

Supplementary characteristics of anti-MHC class II monoclonal antibodies elicited by an ALL cell line: immunofluorescence cytofluorometry, C-dependent cytotoxicity, two-dimensional analysis of antigen.

Monoclonal antibodies directed to MHC class II antigen(s), elicited by a non-T, non-B ALL cell line, were characterized by immunofluorescence flow cytofluorometry and ELISA immunofiltration measurements of their immunoreactivity with selected neoplastic hemopoietic cell lines, determination of their complement-dependent cytotoxic activity against isolated peripheral blood B and T lymphocytes and by two-dimensional electrophoretic analysis (isoelectric focusing, SDS-PAGE) of radiolabeled, immunoprecipitated by these antibodies cell surface antigens. Patterns of these immunological reactivities, as well as two-dimensional radioimmunoprecipitation patterns (acidic heavy chain p35 and basic light chain p30) of antigens recognized by these antibodies confirm their anti-MHC class II specificity. One of these antibodies (braFB6; IgG2b) displayed identical pattern of expression on cell lines and cell types as the typical anti-MHC class II antibodies, but immunoprecipitated only a single chain p30 radioiodinated cell surface protein (with two-dimensional pattern close to the beta-chain of MHC class II DR antigen). These properties indicate the ability of braFB6 monoclonal antibody to recognize a nonpolymorphic determinant of DP-MHC class II antigen.

Antibodies, Monoclonal↗
PubMed1986

The characteristics of the HLA antigens determined by cellular techniques.

The complexity of the D region of the HLA complex is rather great. Three loci--HLA-DP, DQ, DR are very well established in this time. However, the question about the independence of the first known locus of this region--HLA-D--remains elusive--it is still not known if HLA-D antigens are really independent or they rather represent some epitopes of HLA-DR/DQ antigens. The HLA-DP antigens represent the additional series of specificities of the HLA complex recognized by PLT technique. On the other hand HLA-PL antigens, detected by PLT too, correlate as to HLA-D as to HLA-DR antigens and with a great probability they do not form an independent group of HLA antigens.

Antibodies, Monoclonal↗
PubMed1986

The HLA class II antigens determined by cellular techniques. A review.

The D region of the HLA system is very complex. Three loci, HLA-DR, DQ and DP, are now very well established. However, the question of the independence of the first known locus of this region remains elusive--it is not yet known if HLA-D antigens are really independent or if they represent some epitopes on the molecules of HLA-DR/DQ antigens. The HLA-DP antigens represent an independent series in the HLA system recognized by the PLT technique. On the other hand, the HLA-PL antigens also detected by PLT correlate with both HLA-D and HLA-DR antigens, and it seems that they do not constitute an independent group of HLA antigens.

Genes, MHC Class II↗