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M Busacca

Publications and source records attributed to M Busacca.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Immunomagnetic separation of antibody-labelled from antibody-free human spermatozoa as a treatment for immunologic infertility. A preliminary report.

A method is described where superparamagnetic polymer microspheres coated with monoclonal antibodies are used to isolate antibody-labelled from antibody-free spermatozoa in male autoimmune infertility. Autoimmune sperm samples or antibody-free spermatozoa adsorbed with antisperm-antibodies from sera were incubated with microspheres coated with a specific monoclonal antibody to murine immunoglobulins, after their preincubation with mouse anti-human IgG and IgA. Using a magnet, the microsphere-labelled spermatozoa were separated from the samples. Immunobead binding was performed before and after the treatment in order to detect changes in the percentage of antibody-bound spermatozoa. After the immunomagnetic separation, approximately 50% of the IgA-labelled spermatozoa was isolated while no difference was demonstrated when antisperm antibodies of IgG class were involved. The evaluation of sperm motility and membrane integrity after treatment seemed to indicate that the technique did not have any relevant effect on sperm characteristics. The fact that only a partial success in separation of IgA-bound spermatozoa and no success for IgG-labelled sperm was obtained indicates that the method needs to be improved before its clinical utilization might be postulated.

Antibodies, Monoclonal↗
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[Computerized tomography guided biopsy in the diagnosis of neoplastic and inflammatory lesions of the pelvis].

PURPOSE: To assess the efficacy of percutaneous CT-guided biopsy in the diagnosis and therapeutic planning of neoplastic and flogistic diseases of the pelvis. MATERIAL AND METHODS: From July 1990 to December 1999 193 patients (113 males, 80 females: mean age 49, standard deviation 16) were submitted to CT-guided percutaneous biopsy of the pelvic region; 117 biopsies (61%) were performed at iliac, pubic and ischial segments and 76 (39%) at sacral region; 107 patients were admitted to the hospital and 86 were in clinic. Needles were 8 G (4 mm), 10 to 15 cm long. Approach to pelvic lesions was performed according to the specific site. Lesions of the lateral pelvic region have always been approached through the lateral surgical incision according to Enneking. Lesions of the posterior pelvic region have always been approached by the introduction of the needle along the posterior surgical incision according to Enneking. Lesions of the anterior region have always been approached through the anterior surgical incision according to Enneking. From July 1990 to May 1997 pelvic percutaneous biopsies have been carried out with a CT Sytec 3000. From May 1997 to December 1999 the device was replaced by a High Speed CTi. The introduction of spiral CT allowed reduction of performance mean time from 45 minutes (standard deviation 15) to 30 minutes (standard deviation 10). RESULTS: In 154 patients (80%) we observed a neoplastic, inflammatory or not classified degeneration. In 8 patients (4%) the retrieved material ended to be inadequate for a diagnosis. In 31 patients (16%) no disease was revealed at the histological examination. Such patients with negative histological examination have been kept under clinical and radiological control in the following period in order to verify the manifestation or the presence of an alteration previously not observed. On 31-3-2000 none of them had been submitted to a new percutaneous biopsy of the pelvic region. The overall mean accuracy has been 96% considering the negative patients as really negative. In 5 cases (2.6%) we have had complications represented by pain at the introduction and penetration site of the needle. DISCUSSION AND CONCLUSIONS: The choice of the needle, the approach to the lesion and the position of the patient are conditioned by the site of the tumor, its extension, the distance skin-neoplastic disease and by the respect of the incision lines of Enneking, in order not to complicate the job of the orthopaedic surgeon spreading tumoral cells outside the chosen surgical approach. The mean time of the procedure is 30 minutes (standard deviation 10). There are no absolute contraindications to percutaneous biopsy except the suspect or the presence of an hydatideal cyst. The risks have to be compared with those correlated with alternative methods or with the more concerning risk of a missed diagnosis. Complications in the literature range from 0% to 10%, the incidence varying according to the location; pain is the most frequent complication. Altogether the most negative event, although not a true complication, is the retrieval of an inadequate sample: the only drawback of percutaneous biopsy in comparison with incisional biopsy. The accuracy rate of percutaneous biopsy varies in relation to the involved anatomical region, to the pathological process, to the experience of the user, to the amount of the retrieved tissue and to the cooperation of the patient. Our experience shows that, in selected patients, percutaneous biopsy is a virtually safe and almost painless procedure which saves the patient from a surgical procedure in regional or general anestesia as for an incisional biopsy, and allows immediate planning and scheduling of a correct therapy for primitive or secondary neoplastic lesions.

Biopsy↗
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Quiz. Ependymoma.

Explore the source record for details and available documents.

Adult↗
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Osteoid osteoma: diagnostic problems in joint and periarticular localizations.

The diagnosis of osteoid osteoma is usually not difficult. In typical forms of the disease, with diaphyseal localization, the clinical course and radiographic findings are so typical that diagnosis may be considered certain even when there is no histological confirmation. In some localizations, nonetheless, such as in the cancellous or short bones, or when the lesion involves a joint, diagnosis may be difficult, uncertain and late. Between January 1991 and April 1997 a total of 91 patients who had been diagnosed as having osteoid osteoma were submitted to computed tomography. Of these patients, there were 34 females, and 57 males, and age ranged from a minimum of 2 to a maximum of 69 years. Localizations are reported in Table I. For the purposes of our study, several cases with atypical features have been selected from these. We found it interesting to emphasize the importance of a correct diagnostic procedure with the purpose of accurately identifying the lesion.

Adolescent↗
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Which is the best test to evaluate the integrity of sperm plasma membrane?

The functional integrity of sperm plasma membrane of 87 semen samples collected from fertile and infertile men was assessed by the hypoosmotic swelling test (HOS). The results were correlated with sperm motility and supravital stains using trypan blue and eosin Y. These data indicate that sperm swelling and staining methods evaluate different properties of spermatozoa. Moreover the present report shows that staining percentages with trypan blue are significantly lower than with eosin Y.

Cell Membrane↗
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Spinal cord compression by extramedullary hematopoietic tissue in a thalassemic patient: prompt effect of radiotherapy.

As described in the literature, spinal cord compression by extramedullary hematopoietic tissue rarely occurs in thalassemic patients. Laminectomy and/or radiotherapy are the main approaches. We report on a patient with thalassemia intermedia who developed paralysis of both lower extremities due to the compression of the spinal cord by extramedullary hematopoietic tissue.

Adult↗
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Comparison between immunobead test, indirect immunofluorescence and a quantitive ELISA for the diagnosis of antisperm antibodies.

Immunobead Test, Indirect Immunofluorescence and ELISA, have been used to research antisperm antibodies in serum of 105 fertile male patients and in serum of 109 infertile male patients. Antisperm antibodies were present among 12.8% of infertile patients and 0.9% of infertile patients. The immunological factor is certainly an important factor in the aetiology of male hypofertility.

Autoantibodies↗
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Evaluation of antisperm antibodies in infertile couples with immunobead test: prevalence and prognostic value.

We know that antisperm antibodies can cause infertility. We studied the prevalence of the immune response against spermatozoa in infertile couples using immunobead test. 16.2% of the men were autoimmune and 7.3% of the women isoimmune. Both partners were immune in 1.6% of the couples. We also studied the degree of impairment of sperm penetration into cervical mucus in couples in which one of the partners exhibited immunity and we found that generally it correlates with the proportion of sperm exhibiting surface-bound immunoglobulins. In some cases the sperm penetration into cervical mucus was normal in spite of immunization. So other mechanisms of interference should be explored. We found a significant difference (p less than 0.02) in the conception rate between immune and non immune couples (19.3% vs 42%). The pregnancy outcome of immune couples was favorable only in 50% of the cases.

Antibodies↗
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Hysteroscopic pictures following danazol therapy in endometrial hyperplasia.

Danazol is a synthetic steroid derived from ethisterone. Ten women with menometrorrhagia have been treated in order to demonstrate the modifications induced by Danazol on hyperplastic endometrium. Histological and hysteroscopic control demonstrated that Danazol Therapy, at a dose of 200 mg/die, is an efficacious treatment for endometrial hyperplasia.

Adult↗
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Treatment of idiopathic oligozoospermia with tamoxifen.

Eighteen subfertile men, with idiopathic normogonadotropic oligozoospermia were treated with an antiestrogenic compound, tamoxifen (Nolvadex), at the dose of 20 mg/day for four months. Hormonal parameters (LH, FSH, Testosterone, Prolactin) were evaluated before treatment and after 45 and 90 days of therapy. Serum LH, FSH and Testosterone increased significantly after 45 days of tamoxifen treatment. Seminal analyses, performed before and after three months of therapy showed improvements in sperm motility and in sperm density. By our clinical findings, tamoxifen can be considered a useful approach for an empiric treatment of idiopathic oligozoospermia.

Adult↗
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[Study of torsion defects of the lower limbs using computerized tomography].

January, 1990, to July, 1993, the torsional defects of the lower limbs were studied at the Department of Radiology of the Istituto Ortopedico Rizzoli, Bologna. Ninety-three patients were examined, all of them affected with congenital or acquired (posttraumatic and/or iatrogenic) torsional defects. The torsional angles, i.e., the anteversion acetabulum angle, the anteversion femoral neck angle and the tibial torsion angle, were measured as follows: the patients were laid supine with their limbs either in intrarotation with the patella perpendicular to the table (62 patients) or in neutral rotation with the feet in the pace position (31 patients). The images were always analyzed at the console by two different radiologists in the following days. The electronic lines for measuring torsional angles were drawn by the two radiologists ex novo on the previously acquired CT images. No statistically significant differences were observed between the two groups of patients. The measures were independent of limbs position and the interobserver differences were bigger in children and in the measurement of femoral neck anteversion angle; however, these differences decreased by about 50% with better console use. CT, thanks to its feasibility, has replaced conventional (direct or indirect) radiology to study the torsional defects of the lower limbs. Moreover, CT is extremely useful not only for early disease diagnosis (location, rotation degree and associated joint deformities), but also for treatment planning, be it surgical or conservative.

Adolescent↗
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Modern diagnostic imaging of lumbar spondylosis.

The authors retrospectively analyzed 205 patients affected with low back pain and submitted to conventional radiology, CT scan, MR. The purpose of the study was that of gaining a better understanding of the mechanisms caused by the occurrence of spondylosis and its evolution. In light of the most recent literature, the authors conclude: 1) the degenerative process nearly always initiates with laceration of the anulus; 2) dysfunction of the disc then has repercussions on the posterior structures at varying times and with different modalities; 3) symptoms do not always coincide with radiological findings. Thus, the authors believe that for a more complete understanding of the etiopathogenesis and the evolution of spondylosis, further studies involving long-term follow-up of a young population, in relation to clinical and MR findings are needed. MR is the method of choice in determining disc degeneration.

Adult↗