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Biomedical subjects

M Butz

Publications and source records attributed to M Butz.

33 records · Page 2Linked to original sources
PubMed1982

[Oxalate stone prophylaxis by alkalinizing therapy (author's transl)].

Hypocitraturia was found in 75% of oxalate stone formers, and combined with hypercalciuria in 27%. By short-term alkalinizing therapy (3 weeks) with sodium-potassium-citrate (Uralyt-U) a 118% increase in citrate- and a 29.5% decrease in calcium excretion could be achieved in 71 patients. There was no change in the 24 h urinary excretion of oxalate, urate, magnesium and phosphate. In 10 recurrent oxalate stone formers long-term (10 to 20 months) alkalinizing therapy was performed. The quantitative effect on the excretion of citrate and calcium remained unchanged. Seven patients, who have completed at least one year of therapy have had no recurrence of stones.

Alkalies↗
PubMed1981

Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase.

A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.

Aldehyde Dehydrogenase↗
PubMed1980

Oxalate urolithiasis: significance of serum and urinary oxalate.

With a new enzymatic test using oxalate oxidase, serum and urinary oxalate can easily and quickly be determined. Serum oxalate in females was significantly higher than in males (39.5 mumol/l, 30.8 mumol/l). Increased serum levels were found only in male patients. Urinary excretion did not reveal sex-dependent differences in healthy persons. The normal range of oxalate excretion in 104 adult healthy persons was 83-365 mumol/day (95% quartile). In 130 stone formers (nonrecurrent and recurrent group) urinary oxalate excretion was found to be in the normal range. Evaluation of urinary oxalate concentration in morning samples showed increased levels in both groups of male stone formers. Oxalate concentration was unaltered in female patients.

Adult↗
PubMed1980

Dietary influence on serum and urinary oxalate in healthy subjects and oxalate stone formers.

With a new enzymatic method, the dietary influence of oxalate, glycine, protein, and ascorbic acid on serum and urinary oxalate has been examined. Healthy and oxalate stone-forming subjects were compared. Two doses of sodium oxalate (130 and 400 mg daily) were administered. The high dose induced significant hyperoxaluria. No changes of serum oxalate were seen. Neither glycine (4.5 g daily) nor protein (50 g daily, 50% animal protein) had any effect on serum or urinary oxalate. Urinary oxalate excretion did not increase upon ingestion of large amounts of ascorbic acid (1--6 g daily), but serum oxalate levels were significantly elevated. The value of severe dietary restrictions concerning the compounds examined here seems to be questionable, as a significant increase of urinary oxalate excretion is lacking.

Ascorbic Acid↗
PubMed1979

Determination of oxalate in urine using oxalate oxidase: comparison with oxalate decarboxylase.

The oxalate content of urine is determined by means of oxalate oxidase and simple pH measurement. The enzyme specifically decarboxylates oxalate, producing two moles CO2 per mole oxalate. The CO2 diffuses into an alkaline buffer solution (Hallson, P. C. & Rose, G. A. (1974), Clin. Chim. Acta 55, 29--39) in the closed reaction vessel, and reduces the pH value, which is measured with an electrode. Only 125 microliter native urine is required to measure oxalate concentrations in the range of 80 mumol/l to 1.6 mmol/l (corresponding to 7 to 144 mg anhydrous oxalic acid per liter). The limit of detection is 10 nmol oxalate, and the accuracy is 101% with a coefficient of variation of 6%. The method described is insensitive to various interfering factors, such as reducing and oxidizing substances, cloudy or colored samples. It is therefore also suitable for oxalate determination in food technology and plant breeding.

Carboxy-Lyases↗
PubMed1978

Orthotopic and heterotopic autotransplantation of the rat kidney.

End-to-end anastomosis of the kidney vessels and ureter can easily be done without suture. Artery and ureter are connected via an intraluminal silicone tube, anastomosis of the vein is performed with a cuff-technique. In case of orthotopic transplantation the left kidney is transplanted to the right side after right nephrectomy. Furthermore heterotopic transplantation was tried: kidney vessels were either connected with jugular or femoral vessels. In contrast to orthotopic transplantation higher thrombosis rate was observed, probably due to varying sizes of donor and recipient vessels. Our method of orthotopic autotransplantation is easier, less time consuming and has a lower complication rate than homologous kidney transplantation with suture techniques.

Animals↗
PubMed1976

Denudation of the urinary bladder mucosa in the cat by formaldehyde.

Chemical Stripping of the urinary bladder mucosa was studied in 38 cats using 5 to 25% formaldehyde solutions. The contact time varied from 1 to 20 min. With a 20% solution and contact time of 1 min, total denudation was possible without necrosis of subepithelial layers. In such cases, complete reepithelialisation and normal bladder dynamics were seen within 3-4 weeks after formaldehyde instillation. Signs of formaldehyde intoxication due to vesical resorption were not observed.

Animals↗
PubMed1975

Replacement of urinary bladder wall in the cat by autologous x-ray treated full thickness skin graft.

A partial urinary bladder resection was performed in 25 female cats. The defect was replaced with an X-ray treated autologous full thickness skin graft. This graft was shown to be an appropriate foundation for the rapid regeneration of transitional cell epithelium which was proven to be present after 4 weeks. Smooth muscle fibres were present in all cases after 12 weeks. Postoperatively, bladder capacity was adequate without evidence of incrustation or ossification. No changes were seen in the upper urinary tract. Compared to other types of autologous bladder wall replacement this method is simple and warrants clinical investigation.

Animals↗
PubMedDate not supplied

Neuropeptide content of peripheral nerve in relation to nerve function in neuropathy.

OBJECTIVE: In this study an APAAP (alcalic-phophatase-anti-alcalic-phosphatase) technique was used to distinguish afferent (calcitonin-gene-related-peptide (CGRP) or substance-P-(SP) positive) and autonomic (tyrosin-hydroxylase (TH), neuropeptide Y (NPY) or vasoactive-intestinal-polypeptide- (VIP) positive) nerve fibers in sural nerve biopsy material from patients with moderate sensory neuropathy. A panneuronal marker against protein-gene-product 9.5 (PGP 9.5) was used for detection of the total amount of nerve fibers. Second aim was to analyze possible correlations between the impairment in tests for the function of unmyelinated fibers (i.e. thermal threshold testings, sensitivity to painful mechanical stimulation, axon reflex-mediated flare reaction and sudomotor activity) and nerve pathology. RESULTS: A high correlation between CGRP and SP (p < 0.00003) and between TH and NPY, respectively, (p < 0.004) was found, but not between afferent and autonomic markers or between specific markers and PGP 9.5. While no correlations between sensory neuropeptides (CGRP and SP) and specific testings of afferent fiber function or between neuropeptide content and clinical data could be demonstrated, there was a significant correlation between the TH content of the sural nerve and the sweat output, stimulated by acetylcholine iontophoresis at the level of the foot (p = 0.019) and upper leg (p = 0.011). CONCLUSION: This study demonstrates the possibility of visualizing subgroups of unmyelinated nerve fibers in sural nerve biopsies selectively with this technique. The density of TH-positive sympathetic nerve fibers, but not the density of afferent c-fibers, is correlated with corresponding results in specific tests of c-fiber function.

Adult↗