Biomedical subjects

M C B S M Montenegro

Publications and source records attributed to M C B S M Montenegro.

3 recordsLinked to original sources

PubMed2005

Application of sequential injection analysis to pharmaceutical analysis.

Sequential injection analysis is a well established tool for automation of pharmaceutical analysis. A short historical background of this technique is given as well as a brief discussion on the basic principles and potentials. The current applications of SIA in the pharmaceutical analysis are also described and discussed. The manifolds developed offer good analytical characteristics and are suitable for analysis of drug formulations, process analysis, drug-dissolution, drug-release testing and functional assays for screening potential drugs. The results obtained are in good agreement with those furnished by the application of the reference methods presented in the pharmacopoeias.

Biological Availability↗

PubMed2005

New PVC nitrate-selective electrode: application to vegetables and mineral waters.

The paper refers the analytical potentialities of the oxa-azamacrocycles as potentiometric ionophores for the construction of electrodes selective to nitrate. Afterward, the membrane selective to nitrate is designed and optimized using a [3.3.3.3]oxazane as an ionophore. The membrane was prepared using dibutylphthalate as a solvent mediator, tetraoctylammonium as a lipophilic membrane additive, and poly(vinyl chloride (PVC) as a polymeric matrix, applied directly onto a conductive graphite/epoxy resin support. The electrodes presented a slope of 60 +/- 0.1 mV log(-1), a low limit of linear response of 4.2 x 10(-6) mol L(-1), a useful lifetime of 1 year, and improved selectivity characteristics when compared with other nitrate electrodes. The good working characteristics of this electrode, constructed without inner-reference solution, made possible its application to the determination of nitrate in different types of vegetables and bottled mineral waters without the use of a conditioning solution. The application of a significant F test proved that the results obtained were similar to those attained by application of the brucine spectrophotometric method adopted as a reference technique. Linear regression analysis also showed good agreement between the results obtained by the proposed method and the reference one.

Chlorides↗

PubMed2003

Amperometric biosensor based on monoamine oxidase (MAO) immobilized in sol-gel film for benzydamine determination in pharmaceuticals.

The development and application of a flow-through amperometric biosensor for benzydamine determination in anti-inflammatory drugs is described. The biosensor was obtained by physical entrapment of monoamine oxidase in a sol-gel film applied on platinum or carbon paste conducting support. The sol-gel membranes were prepared using an optimum concentration of 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxy silane, double distilled water saturated with polyethylene glycol 6000 and HCl. The developed biosensors were incorporated in a single channel flow injection system to enable the determination of benzydamine in the concentration range of 0.05-2.5 mmol l(-1) (with platinum based electrode) or within 0.1-2.5 mmol l(-1) (carbon paste based electrode). The operational stability of the bioanalytical system developed was about 3 months permitting approximately 4700 substrate measurements. The flow injection system developed enables a sampling rate of 20-25 samples h(-1) and relative S.D. of results less than 4%. The analytical usefulness of the proposed procedure was evaluated through analysis of commercial pharmaceutical products containing benzydamine, available on the Portuguese market. The results obtained did not differ significantly from the values resulting from analysis of the same products by the method described in the BP Pharmacopoeia.

Benzydamine↗