Biomedical subjects

M C Bowman

Publications and source records attributed to M C Bowman.

94 records · Page 6Linked to original sources

PubMedDate not supplied

Chromatographic assays for traces of potentially carcinogenic metabolites of two azo dyes, Direct Red 2 and Direct Blue 15, in rat, hamster and human urine.

Specific, precise and sensitive methods are described for determining traces of potentially carcinogenic metabolites of Direct Red 2 and Direct Blue 15 in rat and hamster urine and to monitor the urine from workers who may be occupationally exposed to these dyes. This methodology is generally applicable to metabolites of azo dyes based on benzidine or three of its congeners. The benzidine-congener free amines, their mono and diacetylated analogs and alkaline hydrolyzable conjugates were determined after appropriate extraction and hydrolysis by HPLC or EC/GC. Residues of metabolites in rat, hamster, and human urine were determined at levels as low as 1 ppb. Supplementary information is also presented concerning hydrolysis of diacetylated metabolites and the stability of Direct Red 2 and Direct Blue 15 in rat, hamster and human urine.

Animals↗

PubMedDate not supplied

Metabolism of a dimethylbenzidine-based dye in rats and hamsters as determined by analysis of the urine for potentially carcinogenic aromatic amines.

Direct Red 2 was given as an aqueous solution to rats and hamsters to determine whether the dye is cleaved to potentially carcinogenic aromatic amines. Sensitive and specific assays of the urine from treated animals by EC/GC revealed appreciable levels of 3,3'-dimethylbenzidine, mono- and di-acetyldimethylbenzidine, and alkaline hydrolyzable conjugates. Peak concentrations of the metabolites in urine occurred 12 to 24 hours after administration to rats, and within 12 hours in hamsters. The levels of all metabolites and conjugates diminished rapidly in both species after peak concentrations were reached, with no residues detected after 96 hours. The results conclusively demonstrated in vivo cleavage of the dye in both species, and it is proposed that the analytical methods employed can be used for chemical monitoring of human urine.

Amines↗

PubMedDate not supplied

Metabolism of nine benzidine-congener-based azo dyes in rats based on gas chromatographic assays of the urine for potentially carcinogenic metabolites.

Metabolism experiments were conducted with rats dosed with nine azo dyes based on dimethyl-, dimethoxy-, or dichlorobenzidine to determine whether the free amine congeners, their monoacetyl or diacetyl metabolites, or alkaline hydrolyzable conjugates were excreted in the urine. After preliminary tests of the dyes, 2-mg doses were administered to each animal and urine samples were collected at intervals up to 96 hours. EC/GC procedures were based on the analysis of heptafluorobutyryl derivatives of the free amine congener moieties or their monoacetyl metabolites. Peak levels of metabolites were excreted either 0-12 or 12-24 hours after administration and, in seven of nine instances, no metabolites persisted in the urine after 48 hours. Minimum detectable levels of all metabolites were 12 ppb or less. All nine dyes were shown to be converted to measurable levels of their benzidine-congener-based metabolites in rats.

Amines↗

PubMedDate not supplied

Spectrofluorometric determination of BAY Vp 2674 residues in poultry tissues.

A spectrofluorometric (SPF) method is described for determination of residues of BAY Vp 2674 in chicken and turkey tissues. The drug is extracted from tissues with dichloromethane-methanol. The organic extract is concentrated to near dryness and cleaned up by a series of partitionings with n-hexane, then dichloromethane against pH 2 buffer and dichloromethane against pH 12 buffer. The drug is partitioned into dichloromethane from pH 7 buffer and concentrated to dryness. The residue is dissolved in pH 3.5 buffer for SPF analysis at 282 nm (excitation) and 445 nm (emission). Recoveries of BAY Vp 2674 added to chicken and turkey tissues at levels of 0.05, 0.1, and 0.2 ppm range from 86 to 92% with a coefficient of variation of 3.4-10.1%. Detection limit is 0.02 ppm. A liquid chromatographic confirmatory procedure is also described, with ultraviolet and fluorescence detection.

Animals↗