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Biomedical subjects

M C Finlayson

Publications and source records attributed to M C Finlayson.

7 recordsLinked to original sources
PubMed1995

Effect of enteropathogenic Escherichia coli on adherent properties of Chinese hamster ovary cells.

Enteropathogenic Escherichia coli (EPEC) O111:H2, O119:H6, or O142:H6 caused rapid detachment of Chinese hamster ovary (CHO) cell monolayers within 2 to 4 h of cocultivation. CHO cell detachment was not promoted by nonenteropathogenic E. coli (O125:H4, O126:H27, O157:H7, and O26:H11) and could not be attributed to EPEC production of enterohemolysin or Shiga-like toxins. In contrast, EPEC strains did not promote rapid detachment of Lec1, Lec2, or Lec8 CHO cell monolayers. These CHO cell Lec mutants all express abbreviated glycan sequences on membrane glycoproteins and glycolipids. Although EPEC strains failed to alter the adherent properties of Lec2 cells lacking only terminal sialic acid groups, EPEC adherence to the Lec2 mutant was indistinguishable from that observed with wild-type CHO cells. There was also no significant difference in EPEC-induced actin accumulation or invasion of Lec2 cells. In contrast, EPEC localized adherence to Lec1 and Lec8 mutants, lacking sialyllactosamine (Lec1) or sialic acid and galactose (Lec8) sequences, was reduced by 84 and 93%, respectively. Our results suggest that lactosamine sequences [beta Gal(1-4 or 1-3)beta GlcNAc] not containing sialic acid are sufficient for EPEC adherence, actin accumulation, and invasion of CHO cells. Sialic acid groups, however, may be necessary for EPEC-mediated CHO cell detachment.

Animals↗
PubMed1985

Salmonellae and salmonellosis in captive reptiles.

In a survey of 150 pet reptiles submitted for necropsy, 51% of snakes, 48% of lizards and 7% of turtles were infected with Salmonella. About one third of the positive animals had died due to various manifestations of salmonellosis. Thirty-one Salmonella serotypes were identified including three isolates new to Canada. The public health implications are discussed in view of the restricted popularity of reptiles and their possible infection from domestic agricultural products.

Animals↗
PubMed1980

An institutional outbreak of Salmonellosis due to lactose-fermenting Salmonella newport.

An institutional outbreak of salmonellosis predominantly due to a lactose-fermenting Salmonella newport is described. Control of the outbreak was hampered by delay in the initial recognition of the aberrant strain. On primary culture, salmonellae were detected on bismuth sulfite agar only; colonies that formed on MacConkey agar and Salmonella-Shigella agar could not be differentiated from lactose-fermenting nonpathogenic organisms. The reactions in triple sugar iron were atypical for Salmonella. The lactose-fermenting property was plasmid-mediated and was readily transferable. Phage typing suggested chicken as a possible source of the strain. The need for awareness of the occurrence of such strains of Salmonella that may not be recognized by cultural procedures in common use, the necessity of the routine use of bismuth sulfite agar in procedures for isolation of salmonellae, and the use of lysine iron agar in conjunction with the triple sugar iron agar are emphasized.

Aged↗
PubMed1977

Scleroderma. Complications encountered during dental anaesthesia.

A young woman with scleroderma required a dental extraction for which general anaesthesia was considered appropriate. A cyanosed and painful hand followed the intravenous injection of methohexitone, and an oral laceration occurred during the extraction of a molar tooth.

Adult↗