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Biomedical subjects

M C Kennedy

Publications and source records attributed to M C Kennedy.

At least 91 records · Page 5Linked to original sources
PubMed1974

Properties of pig heart aconitase.

Comparison of pig heart aconitase (Kennedy et al., 1972) with yeast (Candida lipolytica) aconitase (Suzuki et al., 1973) reveals similarities in molecular weight and iron content but not in sulphide content. Comparison with the Mildvan & Villafranca (1971) pig heart aconitase preparation reveals differences in iron ligands, specific activity and other properties; these differences possibly arise from protein association as pig heart protein associates under a variety of conditions. The electron spin resonance spectrum, g 4.25, and the low molar relaxivity, 473m(-1).s(-1), of water H(+) suggest the presence of high-spin Fe(III) unco-ordinated to water in the enzyme. The iron chromophore on acid titration at 320nm gives a curve with an inflexion at pH4.2. Ten of 16 expected thiol equivalents are titrated with p-hydroxymercuribenzoate suggesting the presence of cystine as well as cysteine residues. Inhibition of the activation of inactive (activatable) enzyme is sigmoidally related to the molar ratio, p-hydroxymercuribenzoate/enzyme with 10-11mol of mercurial compound causing complete inhibition. Active enzyme, free from activating reagents, requires high molar ratios of mercurial compound for rapid inhibition. In terms of p-hydroxymercuribenzoate the enzyme then lacks an essential thiol group.

Aconitate Hydratase↗
PubMed1974

Nitrous fume poisoning in coal-miners.

Soon after the introduction of a new type of explosive in the North Staffordshire coal field, a number of coal miners were seen with acute pulmonary oedema (Kennedy, 1960). These and other coal miners who gave a history of excessive exposure to fumes from shotfiring over a number of years, were followed up between 1960 and 1970. It would appear that both acute and chronic illness may result from excessive exposure to fumes from shotfiring. Short, heavy exposures to fumes may result in acute chest illness from which there may be full recovery. However, exposure to fumes over months or years appears to be associated with the development of an emphysema-like condition. Many of the miners who developed acute pulmonary oedema or severe acute chest illnesses also had prolonged exposure to fumes and later developed emphysema (Kennedy), 1970a, 1970b, 1972).

Absenteeism↗