Biomedical subjects

M C Marques

Publications and source records attributed to M C Marques.

9 recordsLinked to original sources

PubMed2005

Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies.

A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis.

Citrus↗

PubMed2002

Phase transition of a two-dimensional binary spreading model.

We investigated the phase transition behavior of a binary spreading process in two dimensions for different particle diffusion strengths (D). We found that N>2 cluster mean-field approximations must be considered to get consistent singular behavior. The N=3,4 approximations result in a continuous phase transition belonging to a single universality class along the D subset (0,1) phase transition line. Large scale simulations of the particle density confirmed mean-field scaling behavior with logarithmic corrections. This is interpreted as numerical evidence supporting the bosonic field theoretical prediction that the upper critical dimension in this model is d(c)=2. The pair density scales in a similar way but with an additional logarithmic factor to the order parameter. At the D=0 end point of the transition line we found directed percolation criticality.

Journal Article↗

PubMed2002

Adenosine A(2A) receptors in portal hypertension: their role in the abnormal response to adenosine of the cranial mesenteric artery in rabbits.

1. Adenosine is a regulator of mesenteric vasodilation involved in auto-regulation and post-prandial hyperemia, but the adenosine receptor subtype involved in this relaxant effect is poorly characterized. We have now pharmacologically characterized this receptor in rabbit mesenteric arteries and investigated how this adenosine receptor response changes in portal hypertensive animals since the adenosine response is decreased. 2. The closest non-metabolisable adenosine analogue, 2-chloroadenosine (CADO), the mixed A(1)/A(2) receptor agonist, 5'-ethylcarboxamidoadenosine (NECA), and the selective A(2A) receptor agonist, 2-[4-(2-p-carbonyethyl)phenylamino]-5'-N-ethylcarboxamidoadenosine (CGS 21680) (1 pM -- 1 mM) relaxed noradrenaline pre-contracted arteries with a rank order of potency of CGS 21680 (EC(50)=20 nM) > or = NECA (60 nM)>>CADO (640 nM). 3. The selective A(2A) receptor antagonist, 4-(2-[7-amino-2-(2-furyl)-[1,2,4]-triazolo[2,3-a][1,3,5]-triazin-5-ylamino]ethyl)phenol (ZM 241385, 100 nM), shifted to the right the CADO concentration-response curve. 4. In portal hypertensive animals, there was mainly a decreased potency but also a decreased efficacy of all tested adenosine agonists compared to normal animals. Concomitantly, there was a decreased adenosine plasma level and a decreased binding density of [(3)H]-CGS 21680 and [(3)H]-ZM 241385 to mesenteric artery membranes from portal hypertensive compared to normal rabbits. 5. These results indicate that A(2A) receptor activation is required for the adenosine-induced mesenteric relaxation and that the decreased density of A(2A) receptors may contribute to the decreased relaxation induced by adenosine of mesenteric arteries in portal hypertensive animals.

Adenosine↗

PubMed2001

Static critical behavior in the inactive phase of the pair contact process.

Steady-state properties in the absorbing phase of the 1d pair contact process model are investigated. It is shown that, in typical absorbing states (reached by the system's dynamic rules), the density of isolated particles rho(1), approaches a stationary value that depends on the annihilation probability (p); the deviation from its "natural" value at criticality rho(nat)(1) follows a power law: rho(nat)(1)-rho(1) approximately (p-p(c))(beta(1)) for p>p(c). Monte Carlo simulations yield beta(1)=0.81. A cluster approximation is developed for this model, qualitatively confirming the numerical results and predicting beta(1)=1. The singular behavior of the isolated particles density in the inactive phase is explained using a phenomenological approach.

Journal Article↗

PubMed2001

Critical behavior of a nonequilibrium system with two nonordering conserved fields.

We investigate the critical behavior of a nonequilibrium system with two particle species A and B that exhibits a continuous absorbing-state phase transition. The number of particles of each species (N(A) and N(B)) is conserved by the dynamical process. Numerical results show that the order parameter exponent beta depends on the ratio N(B)/N(A) at criticality. Some aspects of critical dynamic behavior are also studied, namely, the decay of the active density at criticality and the critical spreading of a perturbation to an absorbing configuration. Anomalies in the relaxation are associated with the presence of different time scales in the dynamics of the model.

Journal Article↗

PubMed1998

[Approach to the reality of life and health at "Recanto de Alegria" community: understanding and defining the strategy of intervention].

This article presents a process of investigation that was developed to identify life and health conditions of a community in order to propose changing strategies to the situation. It took place in Distrito do Grajaú (SP)--Brazil. All families were interviewed through the application of a survey in order to find personal/life conditions, and a health disease process profile. Data reveal a determined profile that was presented to the people to implement integrated actions to solve the main problems/issues at the community.

Adolescent↗

PubMed1997

Magnetic resonance imaging of localized giant cell tumour of the tendon sheath (MRI of localized GCTTS).

The objective of this study was to evaluate the appearance of localized giant cell tumour of the tendon sheath (GCTTS) on unenhanced and Gd-enhanced MR images. MR images of 13 histologically proven cases of localized GCTTS were evaluated for mean size, location, homogeneity and signal intensity (SI) on both T1- and T2-weighted images, and enhancement pattern. All lesions except 1 affected young adults. On T1- and T2-weighted images, lesions showed predominantly low SI equal to or slightly higher than skeletal muscle. On Gd-enhanced T1-weighted images, strong homogeneous enhancement was seen. These findings reflect the underlying histological composition of the lesion; haemosiderin deposition in xanthoma cells, shortening T2-relaxation time, and abundant collagenous proliferation are responsible for low SI on T1- and T2-weighted images. Strong homogeneous enhancement originates from numerous proliferative capillaries in the collagenous stroma. We conclude that these characteristic MR features, together with clinical information, are a valuable diagnostic tool in offering a correct preoperative diagnosis.

Adolescent↗

PubMed1992

[Primary effect of carotid body stimulation on left ventricular inotropism in swine].

The cardiovascular response to carotid body stimulation has been a interesting and simultaneously controversial subject. In fact, several authors described different responses depending on the animal species used (cat, dog, rabbit, monkey), time of stimulation (short-term v. long-term) and experimental conditions and procedures. According to our experience, the response to carotid body stimulation with cyanide, in normal breathing dogs, consists in hyperpnoea, bradycardia with arterial blood pressure fall (by means of longer diastolic periods), followed by a marked rise in arterial blood pressure and heart rate, after curarization. Concerning the inotropic response to carotid chemoreceptors stimulation, there exists some controversial and even antagonic opinions. In fact, some authors describe a positive inotropic effect, others a negative inotropic effect, and others still defend no inotropic response at all. In the present work, the authors try to contribute to the solution of this problem, studying a group of animals (pig) anaesthetised with chloralose, in which the major modulators of the inotropic response to carotid body stimulation (ventilation; heart rate; and ventricular load conditions) were controlled. To the evaluation of cardiac contractile state, indexes derived from the systolic isovolumic phase were used (maximum left ventricular pressure, dP/dtmax; dP/dtmin) in all the animals. In some animals, other indexes were also used (Vmax, Vpm, (dP/dt)/DP40, Tau and PB). The authors concluded that, in the present experimental conditions, the primary response to carotid body stimulation, in the pig, includes a positive inotropic effect.

Animals↗

PubMed1983

The presynaptic effects of aspirin and prostaglandin E2 in the isolated cat heart.

The presynaptic effects of aspirin and prostaglandin E2 (PGE2) were evaluated in the isolated perfused cat heart preparation. Tissue catecholamine stores were studied by labelling the sympathetic nerve endings with [3H]-noradrenaline ([3H]-NA) and the effect of electric neural stimulation (5 Hz, 0.5 ms, 8 V, 60 s) was determined in the presence of drugs which inhibit neuronal or extraneuronal uptake, or antagonize alpha-adrenoceptors. [3H]-NA overflow was significantly increased by electric neural stimulation. Perfusion with 0.1 microM phentolamine increased transmitter overflow. Infusion of 0.1 microM PGE2 reduced the [3H]-NA overflow induced by electrical stimulation. Administration of 1 microM aspirin increased the [3H]-NA overflow and PGE2 prevented this effect of aspirin. The results support the view that PGEs participate in the negative feedback of transmitter release in the isolated perfused cat heart.

Animals↗