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M C Mazeron

Publications and source records attributed to M C Mazeron.

49 records · Page 3Linked to original sources
PubMed1984

[Clinical aspects of cytomegalovirus infection after allogenic bone marrow grafts].

CMV infection is one of the major infection after bone marrow transplantation. CMV viremia was systematically studied in 66 patients with aplastic anemia or leukemia undergoing BMT. 57% patients had CMV viremia with a frequency peak between 7 and 9 weeks after transplant. Clinical symptoms found during viremia were pancytopenia, fever, cytolytic hepatitis. Interstitial pneumonitis was found only in 4 cases. In 3 cases, viremia was not associated with clinical symptoms. Survival was identical to the group of patients without viremia. Viremia was positively associated with the presence of high anti-CMV antibody titer in donor or recipient before transplant, or to a lymphocyte proliferative response against CMV antigens in donor or recipient before BMT. Granulocyte transfusions increased the frequency of CMV viremia. CMV infection was significantly associated with acute and chronic graft versus host disease. The relation showed between these parameters and viremia provides a basis for an accurate diagnosis of CMV infection and a better background for the study of prophylactic or curative treatment of CMV infection.

Anemia, Aplastic↗
PubMed1984

[Use of acyclovir in the prevention of herpes infections after allogenic bone marrow grafts].

In a double-bind controlled study, oral Acyclovir has been compared to a placebo in a series of 39 consecutive patients undergoing bone marrow transplantation. A dose of 200 mg was given every 6 h from day 8 to day 35 after transplantation. Pharmacokinetic studies have shown the good absorption of the drug despite intestinal damage related to chemoradiotherapy or gut graft-versus-host disease (GVHD), there was no sign of toxicity. The protection against herpes simplex virus (HSV) infection was complete in the treated group when compared to the control group even in patients with high anti-HSV antibody titres. The same protection was observed against cytomegalovirus (CMV) infection. The incidence of HSV and CMV was the same in both groups after treatment ended. This study confirms the efficacy of Acyclovir against HSV infection and possibly against CMV infection when it is given prophylactically after bone marrow transplantation.

Acyclovir↗
PubMed1984

Detection of cytomegaloviremia using monoclonal antibodies.

Isolation of cytomegalovirus (CMV) from blood plated on embryonic fibroblasts necessitates a delay of 10-30 days. Using monoclonal antibodies (McAb) we have developed a 96 hour test to diagnose viremia. We produced monoclonal antibodies directed against CMV (strain AD 169). Clone E 13 McAb detected a CMV early antigen in nuclei using indirect immunofluorescence. These monoclonal antibodies recognized reference strains (Davis and Towne) and 20 field isolates of CMV. E 13 McAb were therefore selected for the diagnostic tests. MRC 5 cells grown on coverslips in tubes were inoculated with buffy-coat from 51 blood samples from bone marrow allograft recipients and Guillain Barre polyneuritis, and centrifuged on hour at 4000 g at 36 degrees C. The indirect immunofluorescence test (IIF) was performed using E 13 McAb, 48 and 96 hours post infection. Three viremia were detected within 48 hours and five others within 96 hours. Plasma and buffy-coat were also inoculated onto MRC 5 cells grown in flasks. Flasks were observed daily for CMV cytopathic effect (CPE). CPE appeared within an average of 14 days. When CMV was detected by CPE, the IF tests were always positive within 96 hours after drawing the blood.

Antibodies, Monoclonal↗
PubMed1983

Prophylaxis of herpes infections after bone-marrow transplantation by oral acyclovir.

In a double-blind controlled study, oral acyclovir was compared with placebo in 39 consecutive patients undergoing bone-marrow transplantation. Acyclovir was given at a dose of 200 mg every 6 h from 8 days before to 35 days after bone-marrow transplantation. Pharmacokinetic studies showed good absorption of the drug, despite intestinal damage related to chemoradiotherapy or gut graft-versus-host disease. There was no sign of toxicity. The protection against herpes simplex virus (HSV) infection was complete in the treated group compared with the placebo group even in patients with high anti-HSV antibody titres before transplantation. The same protection was observed against cytomegalovirus (CMV) infection. The frequencies of HSV and CMV infections were the same in both groups after the cessation of treatment.

Acyclovir↗
PubMed1983

[Non-gonococcal urethritis and cervicitis. Isolation of Chlamydia trachomatis and detection of anti-Chlamydia antibodies by indirect immunofluorescence].

The responsibility of Chlamydia trachomatis in non-gonococcal urethritis and cervicitis was investigated in 267 patients of both sexes. It was confirmed in 36.3% of patients with urethritis and 20.9% of patients with cervicitis by isolating C. trachomatis on Hela 229 cells in the presence of cytochalasin B. No clinical feature specific of C. trachomatis infection could be elicited. The patients were tested for total IgM-type serum anti-chlamydia antibodies by indirect immunofluorescence (IF), using as antigen the inclusions formed in Hela 229 cells by an L2 serotype of C. trachomatis. The serological study was also performed in 86 blood-donors used as controls. The diagnostic value of IF serology is limited in lower genito-urinary infections; the presence of specific IgM's correlates well with the isolation of C. trachomatis, but these IgM's are not detected in protracted urethritis or cervicitis. In such cases, the aetiological diagnosis can only be made by isolation of C. trachomatis from the focus of infection.

Adolescent↗
PubMed1983

Circulating interferon in cytomegalovirus infected bone-marrow-transplant recipients and in infants with congenital cytomegalovirus disease.

In a study concerning five CMV-infected bone-marrow-transplant recipients, five congenital CMV diseases and appropriate controls, presence of high levels of circulating interferon (IFN) was demonstrated exclusively during the course of CMV disease. This interferon was predominantly "immune" or gamma interferon (gamma-IFN). These results suggest that during CMV disease the interferon compartment of the immune response is modified.

Adult↗
PubMed1983

Oral acyclovir prophylactic treatment of herpes simplex infection after bone marrow transplantation.

In a double-blind controlled study, oral acyclovir has been compared to a placebo in a series of 39 consecutive patients undergoing bone marrow transplantation. A dose of 200 mg was given every 6 h from day 8 to day 35 after transplantation. Pharmacokinetic studies have shown the good absorption of the drug despite intestinal damage related to chemoradiotherapy or gut graft-versus-host disease (GVHD); there was no sign of toxicity. The protection against herpes simplex virus (HSV) infection was complete in the treated group when compared to the control group even in patients with high anti-HSV antibody titres. The same protection was observed against cytomegalovirus (CMV) infection. The incidence of HSV and CMV was the same in both groups after treatment ended. This study confirms the efficacy of acyclovir against HSV infection and possibly against CMV infection when it is given prophylactically after bone marrow transplantation.

Acyclovir↗
PubMed1983

[Production of monoclonal antibodies against human cytomegalovirus].

Cell fusion of Sp2/0, a murine myeloma derived non-secreting variant, with splenic lymphocytes from Mice immunized against human cytomegalovirus, has originated two stable hybridomas producing monoclonal antibodies (IgG) which react specifically with antigens that appear early in infectious cycle and remain localized in the cell nucleus.

Animals↗
PubMedDate not supplied

Rapid detection of human cytomegalovirus viraemia from small volumes of heparinized whole blood.

Rapid detection of human cytomegalovirus (HCMV) from blood was performed in parallel using inoculation into human fibroblastic cells of both 500 microliters of whole blood and of buffy coat derived from 9.5 ml of blood. Of the 46 samples tested, 20 were positive for HCMV, 18 when the buffy coat was inoculated (90%) and 17 (85%) when whole blood was used. Fifteen samples were positive by both techniques. Four samples gave discordant results: in three cases, viraemia was detected only by buffy coat assay and in one case only by whole blood assay. These results suggest that HCMV isolation from blood should be performed by inoculating whole blood into cell cultures if only small blood volumes can be collected.

AIDS-Related Opportunistic Infections↗