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Biomedical subjects

M C Patricot

Publications and source records attributed to M C Patricot.

At least 37 records · Page 2Linked to original sources
PubMed1989

Production of monoclonal antibodies to dehydroepiandrosterone-sulphate after immunization of mouse with dehydroepiandrosterone-bovine serum albumin conjugate.

Monoclonal antibodies with a much higher specificity for DHA-S than for DHA were obtained from a BALB/c mouse immunized with a non-sulphated DHA-7CMO-BSA antigen. An improved fusion technique using PEG containing 10% DMSO instead of PEG alone increased the number of positive hybridomas. One of the five monoclonal antibodies obtained, showed a high affinity for DHA-S (Ka = 10(10) M-1) and very low cross-reactions with androsterone (0.62%) and androsterone sulphate (0.83%) which made it potentially useful for direct quantitation of DHA-S in human serum.

Animals↗
PubMed1989

Nitrogen-sparing effect of epidural administration of local anesthetics in colon surgery.

A nitrogen-sparing effect of epidural anesthesia has been clearly demonstrated in gynecological and lower abdominal surgery. To determine if epidural anesthesia also has a protein-sparing effect during major upper or mid-abdominal surgery, postoperative nitrogen balance and 3-methylhistidine urinary excretion (an index of skeletal muscle protein catabolism) were measured for 6 days in 28 patients who had undergone colon resection for cancer with general anesthesia (N2O-O2-1% enflurane) either supplemented with low dose fentanyl plus intermittent systemic pentazocine for postoperative pain (n = 13), or the same general anesthetic plus epidural injection of either etidocaine 1% intraoperatively and bupivacaine 0.25% postoperatively (n = 8) or meperidine (n = 7) for 48 hr after skin incision. The cumulative 6-day nitrogen balance and the cumulative 3-methylhistidine urinary excretion were significantly less after epidural injection of etidocaine intraoperatively and bupivacaine postoperatively than in the two other groups. There was a significant correlation between the daily urinary excretion of 3-methylhistidine and the daily nitrogen balance in the three groups. This study suggests that in colon surgery, epidural analgesia with local anesthetics in the postoperative period improves nitrogen balance and this effect takes place partly in the muscle.

Aged↗
PubMed1987

[Immunological assay of chorionic gonadotropin during pregnancy. Multicenter study of 5 reagents].

Five commercial kits for the quantitative determination of human chorionic gonadotropin (hCG) used for detection and monitoring of pregnancy were evaluated. Five kits used two-sites "sandwich" enzymoimmunoassay and monoclonal antibodies. Precision, standardization and sensitivity were tested and practicability was assessed. Good results are obtained for four kits (Beckman had poor results), sensitivity and specificity are good. Practicability of five kits was evaluated but choose was in relation to configuration of laboratory.

Chorionic Gonadotropin↗
PubMed1987

[Changes in several plasma and urinary components in marathon runners].

33 blood and urinary components were titrated in 11 men 5 days before a marathon race (42 km), just before the start of the race, 1/4 hour after the arrival, the following day and 5 days after the race. On arrival, or/and the following day, even still 5 days later, we observed an increase of: natremia, kaliemia, blood proteins, hematocrit, aldosteronemia, plasma renin activity, uricemia, creatininemia, blood cortisol, myoglobinemia, blood lactic acid, total enzymatic CK activity, enzymatic ASAT activity, urinary elimination of creatinin, urea and 3 methylhistidine. On the contrary, a decrease of plasma total CO2 and blood testosterone levels were observed. These biochemical modifications are the consequence of hydro-mineral losses, muscular necroses, alteration of energetic metabolism with increase of the protein catabolism.

Adult↗
PubMed1987

[Urinary elimination of urinary tetrahydroaldosterone measured by gas-phase chromatography. Normal values in adults].

The authors describe a method of titration of tetrahydroaldosterone in the urine, requiring an internal non-radioactive standard. This method uses high performance liquid chromatography as a mean of isolating tetrahydroaldosterone (THAldo) and chromatography in gaseous phase as a method of titration in the form of heptafluorobutyrate after oxidation into gamma lactone. It permits to detect 3 ng of gamma lactone of THAldo corresponding to a urinary concentration of 16 nmol/l.

Adult↗
PubMed1986

[Comparison between the value of urinary free cortisol and 17-hydroxycorticosteroids for the diagnosis of Cushing's syndrome].

The usefulness for the diagnosis of Cushing's syndrome of urinary free cortisol and urinary 17-hydroxycorticosteroid (17-OHCS) determination was compared by measuring these substances in 17 normal and 28 obese subjects and in 19 patients with established Cushing's syndrome. These measurements were repeated in 16 obese subjects and 11 Cushing's syndrome patients after oral administration of dexamethasone (2 mg/day for 2 days, then 8 mg/day for 2 days). As a group, the patients with Cushing's syndrome had higher (p less than 0.001) excretion rate of both free cortisol and 17-OHCS, but the values observed in this group overlapped with those of normal and obese subjects. The overlap was not significantly different for free cortisol and 17-OHCS excretion. In each obese subject, oral dexamethasone decreased the urinary excretion rate of both free cortisol and 17-OHCS. Seven out of the eleven patients with Cushing's syndrome had no decrease of urinary free cortisol or 17-OHCS, whereas both values were slightly lower in one and decreased to levels similar to those obtained in obese subjects in the three remaining patients. It is concluded that there is no advantage in measuring free cortisol excretion instead of 17-OHCS excretion, either before of after a pituitary-adrenal suppression test, for the diagnosis of Cushing's syndrome.

17-Hydroxycorticosteroids↗
PubMed1986

[Effects of intermittent muscle stimulation on muscle catabolism in patients immobilized in the ICU].

Are muscular contractions obtained by electrical stimulation able to reduce muscle catabolism in immobilized patients? Ten patients (65 to 79 yr old), hospitalized in an intensive care unit for postoperative failure or cerebral infarction, were studied during nine days. Artificial nutrition was the same for each patient during the study. Two periods of four days where defined and randomized for each patient, separated by one day; during the stimulation period (S), intermittent electrical stimulation of the muscles of the legs (external electrodes) was performed daily 2 X 30 min; during the non-stimulation period (NS), muscular stimulation was not performed. Urinary excretion of nitrogen (micro-Kjeldhal digestion and Nessler procedure), creatinine (Jaffé reaction) and 3-methylhistidine were measured every day. Results (X +/- SD) are as follows: the nitrogen balance (g/d) was -1.29 +/- 1.26 during the NS period and 1.43 +/- 1.10 during the S period (NS); 3-methylhistidine (mumol/kg/d) was 3.78 +/- 0.37 during the NS period and 3.15 +/- 0.32 during the S period (p less than 0.01); creatinine (mumol/kg/d) was 92.9 +/- 6.8 during the NS period and 72.9 +/- 25 during the S period (p less than 0.01). It is concluded that a significant decrease in 3-methylhistidine and creatinine excretions is observed during the S period. In intensive care unit patients, muscle protein breakdown may be influenced by intermittent electrical muscle stimulation.

Aged↗
PubMed1985

Urinary 5 alpha-androstane-3 alpha, 17 beta-diol (5-Ad) determination in healthy children and adults.

The authors suggest a method for the evaluation of the urinary 5 alpha-androstane-3 alpha, 17 beta-diol (5 Ad) carried out by gas-liquid chromatography. 5-Ad is separated from its isomers, first by means of an adsorption chromatography on alumina and then by means of a high performance liquid chromatography on porous silica. The reliability criteria of the method have been studied according to the advice of the French Society of Clinical Biology. This method, applied to 175 healthy individuals of both sexes, grouped according to age starting from three years, allowed to determine the usual values of urinary excretion of 5-Ad in relation to age.

Adolescent↗
PubMed1984

Androgen metabolism in vitro by human leukocytes, variations with sex and age.

The present study was undertaken in order to examine the metabolism of androgens by isolated human leukocytes. After incubation, steroids were extracted and purified by high performance liquid chromatography (HPLC); identification and quantification of the steroid products was achieved by gas-liquid-chromatography (GLC), radio-GLC and combined gas chromatography-mass spectrometry (GC-MS) of the trimethylsilyl derivatives (TMS). Incubation in the presence of testosterone led to the formation of 4-ene-androstenedione and 5 alpha-dihydrotestosterone (5 alpha-DHT) while in the presence of 5 alpha-DHT, the products were 5 alpha-androstane-3 alpha, 17 beta-diol (5-Ad) and 5 alpha-androstane-3 beta, 17 beta-diol. The formation of these metabolites was compared in healthy males and females of two age groups. Production of 5 alpha-DHT and 5-Ad was significantly higher in males than in females. In subjects aged 75 years or more, formation of these steroids was decreased by more than half in both sexes, but the sex differences remained. This study confirms the presence in human leukocytes of 17 beta-hydroxysteroid oxydoreductase, 5 alpha-reductase and 3 alpha- and 3 beta-hydroxysteroid oxydoreductase activities.

Adult↗
PubMed1982

[Comparison of radial immunodiffusion and laser nephelometry for the determination of pregnancy specific beta 1-SP1-glycoprotein in plasma].

Concentrations of pregnancy-specific beta 1-glycoprotein were assayed in 137 plasmas by radial immunodiffusion and laser nephelometry. The two methods were evaluated in parallel. A good correlation factor was founded for SP1 values above 60 mg/l. Although the sensitivity of both methods at low concentrations remained unsatisfactory, that of laser nephelometry was superior and therefore to be preferred.

Female↗
PubMed1981

[Immunological quantity determinations of hPL and total oestrogens during the course of pregnancy (author's transl)].

The quantity determination of hPL and of total plasma oestrogens, by means of immuno-enzymology in the heterogenic phase, allows a satisfactory pregnancy. The methods of quantity determination are presented and studied; the criteria for the quality of these methods, and the values found during the course of normal pregnancy, can allow them to be used in many laboratories.

Estrogens↗
PubMed1978

[The deficiency of 17-hydroxylase: a cause of growth and puberty retardation in the girl. One case].

A case of 17-hydroxylase deficiency in a 14 years old girl is reported. She presented with the unique association of hypertension, hypokaliemia, delayed puberty and growth failure. It is suggested that the reduced statural growth starting between 7 and 10 years of age and accompanied by a markedly reduced bone maturation was, at least in part, the consequence of the absence of adrenarche. The secretion rate of cortisol was very low while an increased production of D.O.C. and corticosterone was demonstrated by the elevated levels of their urinary tetrahydroderivatives. A good feminisation and a growth spurt were observed under ethinyl oestradiol substitutive therapy. A progressive normalization of the kaliemia and the blood pressure was obtained with hydrocortisone treatment but long term hydrocortisone therapy appears less satisfactory with easy clinical manifestations of overdosage on the one hand and incomplete maintenance of normal blood pressure of the other hand.

Adolescent↗
PubMed1977

Tolerance to cold and glucose homeostasis in adrenal demedullated dogs.

The rise in O2 consumption and in glucose turnover, induced by acute cold exposure is not suppressed by adrenal demedullation in dogs. However, both at neutral and cold ambient temperature, the mean plasma glucose concentrations are higher in normal (N) than in adrenal-demedullated dogs (ADMX). In the cold, the fall in rectal temperature is larger in ADMX than in N dogs.

Acclimatization↗