Biomedical subjects

M C Peters

Publications and source records attributed to M C Peters.

At least 19 recordsLinked to original sources

PubMed2005

Decrease in inflammatory hyperalgesia by herpes vector-mediated knockdown of Nav1.7 sodium channels in primary afferents.

Induction of peripheral inflammation increases the expression of the Nav1.7 sodium channel in sensory neurons, potentially increasing their excitability. Peripheral inflammation also produces hyperalgesia in humans and an increase in nociceptive responsiveness in animals. To test the relationship between these two phenomena we applied a recombinant herpes simplex-based vector to the hindpaw skin of mice, which encoded both green fluorescent protein (GFP) as well as an antisense sequence to the Nav1.7 gene. The hindpaw was subsequently injected with complete Freund's adjuvant to induce robust inflammation. Application of the vector, but not a control vector encoding only GFP, prevented an increase in Nav1.7 expression in GFP-positive neurons and prevented development of hyperalgesia in both C and Adelta thermonociceptive tests. These results provide clear evidence of the involvement of an increased expression of the Nav1.7 channel in nociceptive neurons in the development of inflammatory hyperalgesia.

Animals↗

PubMed2005

Effect of bur type and conditioning on the surface and interface of dentine.

The purpose of this in vitro study was to evaluate the surface and resin-dentine interface characteristics of permanent tooth dentine cut with diamond or carbide burs and treated with phosphoric acid (PA) or an acidic conditioner. Labial surfaces of permanent incisors were prepared into dentine with high-speed carbide or diamond burs and divided into two halves. Phosphoric acid 36% was applied on one half and non-rinse conditioner (NRC) was applied on the other half. Ten randomly selected scanning electron microscopy (SEM) fields from each specimen (n = 15) were evaluated. Occlusal surfaces of third molars were divided in two halves for evaluation of the resin-dentine interface. The halves were randomly assigned to one of each conditioner and restored with Prime & Bond NT/Spectrum. Ten specimens were analysed by SEM to evaluate hybrid layer formation and interfacial seal. We observed that surfaces prepared with carbide bur presented less residual smear plugs (P < 0.05) than surfaces prepared with diamond burs. Surfaces conditioned with NRC, which is a smear layer modifier, presented more residual smear plugs than surfaces conditioned with PA (P < 0.05). Treatment with PA resulted in more sealed interfaces than specimens treated with NRC. Within the limitations of this study the results showed that carbide burs leave a surface that is more conducive to bonding than diamond burs.

Acid Etching, Dental↗

PubMed2004

Peripheral and central p38 MAPK mediates capsaicin-induced hyperalgesia.

The stress-activated mitogen-activated protein kinase (MAPK) p38 is emerging as an important mediator of pain. The present study examined the possible involvement of peripheral and spinal p38 MAPK in capsaicin-induced thermal hyperalgesia. Topical capsaicin produced phosphorylation of p38 MAPK in the skin from the affected hindpaw as well as the corresponding lumbar spinal cord in a time dependent manner. Topical capsaicin produced robust C-fiber mediated thermal hyperalgesia that was inhibited by systemic, local peripheral, or central intrathecal pre-treatment with the p38 MAPK inhibitor, SD-282. Intraperitoneal SD-282 (10-60 mg/kg) significantly and dose-dependently attenuated capsaicin-induced C-fiber mediated thermal hyperalgesia. Similarly, 0.1-5mg/kg subcutaneous SD-282 in the hindpaw dose-dependently attenuated capsaicin-induced thermal hyperalgesia. Intrathecal administration of 1microg SD-282 was also anti-hyperalgesic in this model. Functionally, SD-282 decreased capsaicin-induced release of calcitonin gene related peptide in an in vitro skin release assay, consistent with a role for p38 MAPK in peripheral nerve function. These results suggest that p38 MAPK plays a role in the development of hyperalgesic states, exerting effects both centrally in the spinal cord and peripherally in sensory C fibers.

Animals↗

PubMed2003

Aging affects two modes of nanoleakage expression in bonded dentin.

Water sorption into resin-dentin interfaces precedes hydrolytic degradation. We hypothesized that these processes are morphologically manifested by the uptake of ammoniacal silver nitrate, which is thought to trace hydrophilic domains and water-filled channels within matrices. Water sorption is thought to be nonuniform and can be traced by the use of silver nitrate. Human teeth bonded with an experimental filled-adhesive were aged in artificial saliva (experimental) or non-aqueous mineral oil (control). Specimens retrieved for up to a 12-month period were immersed in 50 wt% ammoniacal silver nitrate and examined by transmission electron microscopy for identification of the changes in their silver uptake. Reticular silver deposits initially identified within the bulk of hybrid layers in the experimental group were gradually reduced over time, but were subsequently replaced by similar deposits that were located along the hybrid layer-adhesive interface. Silver uptake in water-binding domains of the adhesive layers increased with aging, resulting in water tree formation. These water-filled channels may act as potential sites for hydrolytic degradation of resin-dentin bonds.

Absorption↗

PubMed2001

Engineering and characterization of functional human microvessels in immunodeficient mice.

SUMMARY: Current model systems used to investigate angiogenesis in vivo rely on the interpretation of results obtained with nonhuman endothelial cells. Recent advances in tissue engineering and molecular biology suggest the possibility of engineering human microvessels in vivo. Here we show that human dermal microvascular endothelial cells (HDMEC) transplanted into severe combined immunodeficient (SCID) mice on biodegradable polymer matrices differentiate into functional human microvessels that anastomose with the mouse vasculature. HDMEC were stably transduced with Flag epitope or alkaline phosphatase to confirm the human origin of the microvessels. Endothelial cells appeared dispersed throughout the sponge 1 day after transplantation, became organized into empty tubular structures by Day 5, and differentiated into functional microvessels within 7 to 10 days. Human microvessels in SCID mice expressed the physiological markers of angiogenesis: CD31, CD34, vascular cellular adhesion molecule 1 (VCAM-1), and intercellular adhesion molecule 1 (ICAM-1). Human endothelial cells became invested by perivascular smooth muscle alpha-actin-expressing mouse cells 21 days after implantation. This model was used previously to demonstrate that overexpression of the antiapoptotic protein Bcl-2 in HDMEC enhances neovascularization, and that apoptotic disruption of tumor microvessels is associated with apoptosis of surrounding tumor cells. The proposed SCID mouse model of human angiogenesis is ideally suited for the study of the physiology of microvessel development, pathologic neovascular responses such as tumor angiogenesis, and for the development and investigation of strategies designed to enhance the neovascularization of engineered human tissues and organs.

Absorbable Implants↗

PubMed2001

Polymeric system for dual growth factor delivery.

The development of tissues and organs is typically driven by the action of a number of growth factors. However, efforts to regenerate tissues (e.g., bone, blood vessels) typically rely on the delivery of single factors, and this may partially explain the limited clinical utility of many current approaches. One constraint on delivering appropriate combinations of factors is a lack of delivery vehicles that allow for a localized and controlled delivery of more than a single factor. We report a new polymeric system that allows for the tissue-specific delivery of two or more growth factors, with controlled dose and rate of delivery. The utility of this system was investigated in the context of therapeutic angiogenesis. We now demonstrate that dual delivery of vascular endothelial growth factor (VEGF)-165 and platelet-derived growth factor (PDGF)-BB, each with distinct kinetics, from a single, structural polymer scaffold results in the rapid formation of a mature vascular network. This is the first report of a vehicle capable of delivery of multiple angiogenic factors with distinct kinetics, and these results clearly indicate the importance of multiple growth factor action in tissue regeneration and engineering.

Actins↗

PubMed2001

Quasisteady aero-acoustic response of orifices.

The low frequency response of orifices (slit, circular diaphragm, and perforated plate) in the presence of mean flow is well predicted by a quasisteady theory. A refinement is brought to the theory by considering a Mach number dependent vena contracta coefficient. The measurements of the vena contracta coefficient of a slit agree well with the simple analytical expression existing in the case of the Borda tube orifice. The measured scattering matrix coefficients do not depend strongly on the geometry of the element. If the frequency is increased the moduli remain relatively unaffected while the arguments exhibit a complex behavior which depends on the geometry. From these considerations an anechoic termination efficient at high mass flow is designed.

Journal Article↗

PubMed2001

Minimally invasive operative care. II. Contemporary techniques and materials: an overview.

SUMMARY: Restorative dentistry has experienced a shift from the mainly reparative dentistry of the 20th century towards a minimal intervention approach. Contemporary operative treatment incorporates the MI philosophy in cavity design. Currently available techniques to pursue minimally invasive restorative treatments are highlighted. Characteristics of adhesive materials that facilitate minimally invasive operative care are discussed. CONCLUSION: When operative intervention is the designated treatment for initial caries, currently available operative techniques and contemporary materials warrant a minimally invasive approach. Minimal intervention applied to the operative field keeps the options open for long-term preservation of the restored tooth.

Biocompatible Materials↗

PubMed2001

Minimally invasive operative care. I. Minimal intervention and concepts for minimally invasive cavity preparations.

SUMMARY: From the mainly reparative dentistry of the 20th century, contemporary dentistry shifts towards a minimal intervention (MI) approach encompassing up-to-date caries diagnosis and risk assessment before arriving at a treatment decision. An overview is provided of incorporating MI philosophy into the field of operative dentistry. The ultimate goal of MI is to extend the lifetime of restored teeth with as little intervention as possible. When operative care is indicated, it should be aimed at "prevention of extension." Black's principles for cavity design are considered and put in the perspective of minimally invasive operative care. Guiding principles for contemporary adhesive cavities are reviewed. CONCLUSION: Contemporary operative care should be based on a minimally invasive approach. Minimal intervention is not just a technique, it is a philosophy!

Dental Bonding↗

PubMed2000

Bioabsorbable polymer scaffolds for tissue engineering capable of sustained growth factor delivery.

Engineering new tissues utilizing cell transplantation on biodegradable polymer matrices is an attractive approach to treat patients suffering from the loss or dysfunction of a number of tissues and organs. The matrices must maintain structural integrity during the process of tissue formation, and promote the vascularization of the developing tissue. A number of molecules (angiogenic factors) have been identified that promote the formation of new vascular beds from endothelial cells present within tissues, and the localized, controlled delivery of these factors from a matrix may allow an enhanced vascularization of engineered tissues. We have developed a gas foaming polymer processing approach that allows the fabrication of three-dimensional porous matrices from bioabsorbable materials (e.g., copolymers of lactide and glycolide [PLG]) without the use of organic solvents or high temperatures. The effects of several processing parameters (e.g., gas type, polymer composition and molecular weight) on the process were studied. Several gases (CO(2), N(2), He) were utilized in the fabrication process, but only CO(2) resulted in the formation of highly porous, structurally intact matrices. Crystalline polymers (polylactide and polyglycolide) did not form porous matrices, while amorphous copolymers (50:50, 75:25, and 85:15 ratio of lactide:glycolide) foamed to yield matrices with porosity up to 95%. The mechanical properties of matrices were also regulated by the choice of PLG composition and molecular weight. Angiogenic factors (e.g., vascular endothelial growth factor) were subsequently incorporated into matrices during the fabrication process, and released in a controlled manner. Importantly, the released growth factor retains over 90% of its bioactivity. In summary, a promising system for the incorporation and delivery of angiogenic factors from three-dimensional, biodegradable polymer matrices has been developed, and the fabrication process allows incorporation under mild conditions.

Absorption↗

PubMed2000

Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds for tissue engineering.

Strategies to engineer bone tissue have focused on either: (1) the use of scaffolds for osteogenic cell transplantation or as conductive substrates for guided bone regeneration; or (2) release of inductive bioactive factors from these scaffold materials. This study describes an approach to add an inductive component to an osteoconductive scaffold for bone tissue engineering. We report the release of bioactive vascular endothelial growth factor (VEGF) from a mineralized, porous, degradable polymer scaffold. Three dimensional, porous scaffolds of the copolymer 85 : 15 poly(lactide-co-glycolide) were fabricated by including the growth factor into a gas foaming/particulate leaching process. The scaffold was then mineralized via incubation in a simulated body fluid. Growth of a bone-like mineral film on the inner pore surfaces of the porous scaffold is confirmed by mass increase measurements and quantification of phosphate content within scaffolds. Release of 125I-labeled VEGF was tracked over a 15 day period to determine release kinetics from the mineralized scaffolds. Sustained release from the mineralized scaffolds was achieved, and growth of the mineral film had only a minor effect on the release kinetics from the scaffolds. The VEGF released from the mineralized and non-mineralized scaffolds was over 70% active for up to 12 days following mineralization treatment, and the growth of mineral had little effect on total scaffold porosity.

Biocompatible Materials↗

PubMed1999

Comparison of two measurement techniques for clinical wear.

OBJECTIVES: Clinical wear of restorations is generally evaluated by marginal integrity over time. In this study, both a subjective and an objective method for wear assessment are compared, and the relative advantages and disadvantages of each are considered. METHODS: A surface evaluation technique for quantitative measurement of wear, as developed at the University of Minnesota (UMN) has been compared to the commonly employed method of wear assessment used by Leinfelder (LF). Measurements were made by comparing suitable casts of restored teeth before and after clinical function. Semi-quantitative wear assessment was studied in comparison with detailed quantitative information about the topology of the pre- and post-wear occlusal surfaces obtained from stylus profilometry and processed by imaging techniques. Fourteen model sets of baseline, 2, 3 and 5 year old Class II composite restorations from a Danish clinical trial were evaluated using both techniques. RESULTS: In general, after 5 years of function, the digitizing method (UMN) generated wear values that were twice as high, indicating that general wear of restorative materials is underestimated by the LF-method. CONCLUSIONS: The comparison clearly revealed the advantages and limitations of each technique. Evaluation of wear by means of the LF technique provides global semi-quantitative data on restoration margins relative to enamel, underestimating general wear of the restorative material. Advanced 3-D measuring techniques such as the UMN method provide extensive quantitative data regarding wear patterns of the entire occlusal surface, i.e. restoration and enamel. Such a highly accurate technique is capable of differentiation between wear behavior of restorative materials early on in clinical studies. In addition, through its digital alignment procedure, the UMN method provides data on accuracy of the replication process used in clinical studies.

Analysis of Variance↗

PubMed1998

Dimensional changes during veneering procedures on discoloured teeth.

OBJECTIVES: The aim of the study was to determine the dimensional changes of teeth when veneering procedures are involved. METHODS: Fifteen discoloured central incisors were selected from a group of 180 veneer restorations (VRs) composed of three different types of materials (direct resin composite, indirect resin composite and porcelain). Impressions and dies were made before treatment, after preparation and after placement and finishing the restoration. Contact stylus profilometry and subsequent analysis of the 3-D surface images provided quantitative data of the differences between the various treatment phases. RESULTS: The preparation reduction was the most for indirect resin composite VRs and the least for direct resin composite VRs. All veneer restorations showed nearly the same thickness and dimensional change after treatment, except one, which resulted in an additional increase of volume of the tooth. CONCLUSION: From the results of this study it is concluded that the dimensions of a discoloured tooth treated with a veneer restoration unintentionally increased, resulting in overcontour.

Analysis of Variance↗

PubMed1998

Enamel cracks. The role of enamel lamellae in caries initiation.

Lamellae or cracks are distributed throughout tooth enamel in both deciduous and permanent dentitions. While earlier authors postulated that lamellae may be pathways of entry for caries, no evidence was adduced and the theory appears to have been discounted. The present study seeks to show that, at least in some cases, lamellae are permeable to dyes, may be associated with caries initiated in the dentine, supporting the hypothesis of Hardwick and Manly of lamellae penetration by Streptococcus mutans and lactobacilli. The enamel lamellae are shown to be a permeable pathway allowing caries-producing bacteria access to the dentine-enamel junction. Caries can thus be established within the tooth without visible evidence at the surface.

Azo Compounds↗

PubMed1998

Release from alginate enhances the biological activity of vascular endothelial growth factor.

A primary factor which limits engineering tissues of substantial size is the lack of nutrients readily available to transplanted cells. One potential solution to this nutrient limitation is to encourage the rapid development of a vascular network within three-dimensional tissue engineering matrices. Vascular endothelial growth factor (VEGF) has been identified as a potent stimulator of angiogenesis in vivo. Though effective at stimulating endothelial cells to form blood vessels VEGF degrades rapidly. Spherical alginate beads (3.3+/-0.1 mm diameter) were examined as a means of delivering biologically functional VEGF at a controlled rate over extended times. The alginate beads demonstrated the ability to incorporate VEGF with an efficiency between 30 and 67%, depending on the processing conditions, and release it at a constant rate (5%/day) for up to 14 days in vitro. The released VEGF, when assayed for its ability to stimulate endothelial cells in culture, was found not only to be functional but more potent (three to five times) than the same mass of VEGF added directly to the culture medium. The release kinetics of freeze dried VEGF containing alginate beads were also examined and found to be comparable to non-freeze dried samples.

Alginates↗

PubMed1997

Electron probe microanalysis and transverse microradiography studies of artificial lesions in enamel and dentin: a comparative study.

In cardiology and research involving the interactions between restorative materials and dental hard tissues, it is important for small changes in tooth mineral content to be measurable. Currently, transverse microradiography (TMR) is the most accepted tool for the above purpose. Electron Probe Microanalysis (EPMA) can yield both qualitative identification of elements and quantitative compositional information. The purpose of this study was to compare the mineral distribution in well-defined artificial lesions, in dentin and enamel, by the use of both TMR and EPMA on the same sample. The good correlation between the two sets of data validates EPMA as a technique and helps in the interpretation of its results. The data from TMR analysis are expressed as vol% of mineral, while EPMA gives the levels of Ca and phosphate in wt%. The conversion between the two sets of data is complicated by the fact that local density is, as yet, unknown.

Analysis of Variance↗

PubMed1997

A study of glass-ionomer cement and its interface with enamel and dentin using a low-temperature, high-resolution scanning electron microscopic technique.

This report describes a method of immobilizing the water contained in glass-ionomer cement and dental hard tissues and stabilizing the delicate organic component of dentin. With this method, the intact interface between glass-ionomer and dental hard tissues can be observed under scanning electron microscope with few of the artifacts that are caused by the desiccation associated with conventional scanning electron microscopic studies. There was a distinct zone of interaction between the glass-ionomer cement and enamel and dentin. Under severe thermal stress, glass-ionomer cement failed cohesively, leaving an intact interface with enamel and dentin. Machine-mixed glass-ionomer cements displayed a high level of porosity. Some glass particles were separated from the matrix, and there was evidence that some are dislodged from the matrix during specimen preparation.

Dental Enamel↗

PubMed1996

The strength of auto-cured and light-cured materials. The shear punch test.

This paper examines the versatility of the shear punch test as described by Roydhouse and suggests that it should be considered as an alternative to the present standard compressive strength test for glass ionomer cements and flexural strength for composite resins. The shear punch test can be used for examining small thin specimens, about 1.0 mm thick and 8.0 mm in diameter, of both auto-cured and light-cured restorative materials such as composite resins and glass ionomer cements. The preparation of the specimen is simple and does not require a precision mould or subsequent machining to size except that, after curing, it may need to be abraded gently to obtain flat parallel test surfaces. The test apparatus consists of a punch approximately 3.0 mm in diameter opposing a true fitting matching die. The specimen is supported over the die section and the punch is advanced through it in a compression cage. The formula used to calculate the shear strength allows for variation in specimen thickness and thus provides comparative data between materials. It also allows examination of the effects of variations in manipulation, maturation and storage of each material. It is suggested this test should be considered as an alternative to the present compression and flexural strength tests because it would provide a single strength test for a range of restorative materials being manipulated under a variety of circumstances. It is of particular significance for testing the light-cured materials because it examines a specimen size below that of the diameter of the exit window of a normal clinical light curing unit and also at a thickness where depth of cure of the material is not a problem.

Composite Resins↗