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Biomedical subjects

M C Rees

Publications and source records attributed to M C Rees.

At least 37 records · Page 2Linked to original sources
PubMed1991

Variation during the menstrual cycle of immune cell populations in human endometrium.

Morphometric analysis and immunohistology of tissue sections have been used to assess variation, during the normal menstrual cycle, of the bone marrow-derived cell populations in human endometrium. Levels of T cells and macrophages were found to be relatively constant throughout the cycle. In contrast, numbers of large granular lymphocytes, identified as being CD56-positive, were generally low between days 10 and 19, but increased sharply in the latter part of the luteal phase, decreasing again after menstruation. This LGL population is known to be abundant in first trimester pregnancy decidua, and is presumed to play a role in early pregnancy success.

Antigens, CD↗
PubMed1991

Menopausal flush symptomatology and sustained reflex vasoconstriction.

Reflex vasoconstriction was examined using the digital vasoconstrictor response to ice application in 102 postmenopausal women. Women experiencing hot flushes tended to lack the normal vasoconstrictor response whereas women with lower flush symptomatology tended to have a vasoconstrictor response. There was a significant relationship between the percentage occurrence of a vasoconstrictor response and both the daily flush score (P less than 0.001) and subjective severity of flushes (P less han 0.001).

Adult↗
PubMed1991

Epidermal growth factor receptor in human uterine tissues.

Epidermal growth factor receptor (EGFR) was studied in human endometrium and myometrium collected throughout the menstrual cycle (five follicular, five luteal specimens) by immunohistochemistry and quantitative ligand binding. EGFR was principally present on the cell surface of glandular epithelium of the endometrium. Staining was no different between the follicular and luteal phases. There was more EGFR binding in endometrium than myometrium by quantitative ligand binding in membrane preparations: endometrium, median = 92.5 fmol/mg protein (range 25.3-294) n = 10; myometrium, median = 43.0 fmol/mg protein (range 23.4-57) n = 10. There was no difference in the level of binding in the follicular and luteal phases.

Binding Sites↗
PubMed1990

Human menstruation and eicosanoids.

Menstruation is an event restricted to humans and subhuman primates and in which uterine bleeding and contractions occur. Prostaglandins and leukotrienes are thought to play a major role in the normal process of menstruation as well as in the disorders menorrhagia and dysmenorrhoea.

Dysmenorrhea↗
PubMed1990

Gestrinone in the treatment of menorrhagia.

The role of gestrinone, 2.5 mg twice weekly, in treating proven menorrhagia (greater than 80 ml) was examined in 19 women. They were treated for five cycles (2 placebo, 3 active), taking one capsule twice weekly. Placebo had no effect on menstrual blood loss (MBL). On gestrinone 10 women became amenorrhoeic, in five MBL was markedly reduced (5-74 ml) and four did not respond. In three of the non-responders submucous leiomyomas were found at subsequent hysterectomy. Follow-up periods showed a persistent reduction in MBL for nine women in the first post-treatment menstruation.

Adult↗
PubMed1988

Effect of fenamates on prostaglandin E receptor binding.

The effect of fenamates on prostaglandin E receptor binding was examined in myometrial samples collected at hysterectomy. Sodium meclofenamate and mefenamic acid inhibited binding of PGE2 to its specific receptor in a dose-dependent manner, with an ED50 of 20 mumol/l and 200 mumol/l, respectively.

Dinoprostone↗
PubMed1988

Absence of sustained reflex vasoconstriction in women with menopausal flushes.

Reflex vasoconstriction was examined using the digital vasoconstrictor response to ice application in 10 flushing and six non-flushing menopausal women, 22 premenopausal women and eight men. Nine of the 10 women experiencing flushes lacked the vasoconstrictor response which was present in all the other groups examined, suggesting an abnormality of autonomic nervous control. Our observation is of particular interest since it has previously not been possible to apply any biochemical or biophysical tests which differentiate flushing from non-flushing subjects except during an actual flush.

Adult↗
PubMed1988

Increased uterine prostaglandin E receptors in menorrhagic women.

PGE receptor concentrations were measured in myometrial samples collected from 10 women at hysterectomy. Five women had normal measured menstrual blood loss (35-44 ml) and the remainder had unexplained menorrhagia occurring in the absence of any uterine, pelvic or general pathology, with losses ranging from 85 to 925 ml. Median PGE receptor concentrations were significantly higher in the women with menorrhagia (1077 fmol/mg protein) than in the women with normal menstrual blood loss (625 fmol/mg protein) and correlated with menstrual blood loss (P less than 0.02). These findings suggest that unexplained menorrhagia may simply be a constitutional variant in some women and that specific and potent PGE uterine receptor antagonists would furnish effective non-surgical treatment for unexplained menorrhagia.

Adult↗
PubMed1988

Leukotriene release by human fetal membranes, placenta and decidua in relation to parturition.

Complete placentas and membranes were obtained from women after uncomplicated singleton pregnancies. Six were collected after labour at term, six after preterm labour and six after elective Caesarean section at term. Leukotriene C4 (LTC4), leukotriene D4 (LTD4) and leukotriene E4 (LTE4) release was examined using a short-term incubation technique. Release by amnion was significantly higher than that by the other tissues for the three modes of delivery. Comparison of LTC4, LTD4 and LTE4 release by individual tissues with regard to the type of delivery showed no significant difference.

Cesarean Section↗
PubMed1987

Leukotriene release by endometrium and myometrium throughout the menstrual cycle in dysmenorrhoea and menorrhagia.

Endometrium and myometrium were collected at hysterectomy from 21 women with measured menstrual blood loss. Eight women complained of dysmenorrhea and the remaining 13 had pain-free periods. Specimens were obtained throughout the menstrual cycle (menstrual, n = 5; follicular, n = 4; early luteal, n = 3; mid-luteal, n = 5; late luteal, n = 4). Leukotriene C4, leukotriene D4 and leukotriene E4 release were examined using a short-term incubation technique. Endometrial leukotriene release, which was always significantly greater than myometrial release, changed throughout the menstrual cycle and the highest concentrations were found during menstruation. Endometrial, but not myometrial, leukotriene concentrations were significantly higher in tissues obtained from women with a complaint of dysmenorrhoea compared with those in tissue from pain-free women. No correlation was found between leukotriene release in either endometrium or myometrium and menstrual blood loss (range 15-457 ml).

Adult↗
PubMed1987

Immunohistochemical localization of relaxin, prolactin and prostaglandin synthase in human amnion, chorion and decidua.

Relaxin, prolactin and prostaglandin synthase were localized by the avidin-biotin immunoglucose oxidase method in human amnion, chorion and decidua. Specimens from ten normal spontaneous deliveries and four elective Caesarean section deliveries with no labour were compared. Relaxin was found more consistently in the cells of the chorionic cytotrophoblast than in the cells of the parietal decidua adherent to the fetal membranes. Only half the tissues after spontaneous delivery contained positive relaxin-stained cells, whereas all the tissues from elective Caesarean sections contained cells positively stained with antiserum to relaxin. In both series of tissues prolactin was localized predominantly in the parietal decidual cells and was very infrequently found in the chorionic cytotrophoblast. Polyclonal antiserum to prostaglandin synthase was used to identify those cells producing prostaglandin in amnion, chorion and decidua. The cells of the amnion and chorion showed positive immunolocalization with no differences between tissues collected before or after labour. Double immunostaining using avidin-biotin immunoperoxidase for prolactin, followed by avidin-biotin immunoglucose oxidase for prostaglandin synthase, produced identical results in the same series of tissues examined with the single-staining method.

Amnion↗