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M Cano

Publications and source records attributed to M Cano.

96 records · Page 6Linked to original sources
PubMedDate not supplied

Effect of sulfosulfuron on the urine and urothelium of male rats.

Sulfosulfuron, developed as a herbicide, caused increased microcrystalluria and the formation of urinary tract calculi when fed to male and female rats in a chronic 2-year study at doses of 5,000 ppm and 20,000 ppm. Hyperplasia was also seen in urinary bladders at 5,000 ppm and 20,000 ppm, almost exclusively in the presence of observable calculi/microcalculi. Urinary bladder tumors were found in 2 females in the 5000 ppm group, both in the presence of calculi. No increased microcrystalluria, calculi, or tumors were found at doses of 500 ppm and lower. In the current study, 5 groups of male Sprague-Dawley rats were fed sulfosulfuron at doses of 50, 500, 5,000, and 20,000 ppm for 10 weeks. Ten animals were co-administered 5,000 ppm sulfosulfuron with 12,300 ppm NH4Cl to determine if inhibition of the formation of calculi would prevent any urothelial effects of treatment with sulfosulfuron. Ten animals in the control group and in the high-dose sulfosulfuron group were fed only basal diet for an additional 10 weeks to determine if the effects of sulfosulfuron on the bladder epithelium were reversible. There was an increased incidence of microcrystalluria observed at 5,000 and 20,000 ppm. There was no increase in microcrystalluria observed in the urine of rats co-administered sulfosulfuron and NH4Cl. Urinary bladder calculi were found in the bladder of 1 animal fed 20,000 ppm. Examination by light microscopy showed diffuse papillary/nodular hyperplasia of the bladder epithelium in this animal. No increased microcrystalluria was observed after withdrawal of the chemical from the diet and the bladder epithelium was normal by light microscopy. The hyperplastic effects associated with the feeding of high doses of sulfosulfuron occur only with the appearance of urinary tract calculi. Based on these results and anatomical differences between rats and humans, it may be concluded that the hyperplastic and carcinogenic effects of sulfosulfuron in rats are high-dose, threshold phenomena that are not likely to occur in humans under environmentally relevant exposures.

Ammonium Chloride↗
PubMedDate not supplied

Evaluation of diet and dimethylarsinic acid on the urothelium of Syrian golden hamsters.

Few studies have examined the carcinogenicity of chemicals toward the urinary bladder in hamsters, and the effect of diet on hamster urine and urothelium has not been reported. Our laboratory recently began investigating the effects of dimethylarsinic acid (DMA) on the hamster bladder, and we noticed subtle urothelial changes even in controls. The possible effect of various diets on hamster urothelium was evaluated by feeding different diets to 4-week-old Syrian Golden hamsters for 5 weeks. The diets examined were Tekland 8656, Purina 5002, Purina 5L79, and NIH-07. Light microscopic examination showed a slight increase in urothelial hyperplasia in hamsters fed Purina 5L79. An increase in the incidence of urinary bladder necrosis, exfoliation, and mild hyperplasia were noted by scanning electron microscopy (SEM) with all dietary preparations except NIH-07. The constituents in the diets producing the urothelial alterations are not known at present, but NIH-07 diet was chosen for experiments to investigate the effects of DMA on the hamster bladder epithelium. Male and female 5-week-old Syrian Golden hamsters were fed 100 ppm DMA for 10 weeks. Examination of urinary parameters showed no treatment-related changes. Light microscopic examination and SEM revealed no changes of the urothelium of DMA-treated male or female hamsters.

Animals↗
PubMedDate not supplied

Extensive handling of rats leads to mild urinary bladder hyperplasia.

The urinary and urothelial effects of the frequent handling necessary for obtaining fresh-voided urine specimens were evaluated in 5-wk-old male F-344 rats fed control diet or diet containing 7.5% sodium saccharin. Frequent handling consisted of holding rats by the back of the neck in a position to obtain fresh-voided urine directly into centrifuge tubes 3 times per week for 10 weeks, whereas seldomly handled control rats received this treatment only twice during the entire 10 weeks. The urothelium of frequently handled rats fed control diet showed superficial necrosis and regenerative hyperplasia as observed by light and scanning electron microscopy. These changes were not observed in rats fed control diet that were seldomly handled. The necrosis and hyperplasia were not as pronounced in frequently handled rats fed control diet as in seldomly handled, sodium-saccharin-treated rats, but handling also potentiated the severity of the changes produced by sodium saccharin feeding. The urothelial exfoliation and consequent regenerative hyperplasia are likely secondary effects of stress.

Animals↗
PubMedDate not supplied

[Spontaneous non-hypertensive cerebral hematomas].

A retrospective study of 35 non-hypertensive intraparenchymal brain hematomas (NHBH) is made to evaluate their etiopathogenesis and the therapeutic approaches employed. A comparison is made with the hypertensive hematomas seen during the same period. Among the NHBH there were 5 vascular malformations (VM (14%), 5 hematomas secondary to brain tumors (14%), 2 amyloid angiopathies (6%), 2 cases of coagulation dysfunction (6%), 3 hematomas secondary to anticoagulation therapy (9%), and one hematoma in an intravenous drug abuser (3%). No etiological diagnosis was established in the remaining 17 cases (48%). The NHBH were predominantly lobar (43%), and incidence was greater among young patients. Mortality was 20%. All cases were diagnosed by brain CT, the latter suggesting pathology underlying the hematoma in 3 tumoral hematomas, in 2 VM confirmed arteriographically, and in one patient with suspect VM not confirmed by angiography. A total of 22 angiographies were performed: the latter were diagnostic in all VMs and negative in the tumor hematomas, amyloid angiopathies and in those hematomas of unknown etiology where angiography was performed. Fifteen patients were operated on. We recommend that in patients with brain hematomas but without antecedents of arterial hypertension or with an atypically situated hematoma, a careful examination should be carried out to discard all possible causes of brain hemorrhage, together with the early performing of contrasted CT scans and posterior angiography. If the latter prove negative, the patient should undergo a clinico-radiological follow-up, together with an histological study of the blood clot if operated on.

Adolescent↗
PubMedDate not supplied

[Use of ticlopidine in primary care].

OBJECTIVES: To find the reasons for prescription of Ticlopidine, which acts against the aggregation of platelets and is catalogued as "with hospital diagnosis", and to evaluate the criteria for its correct use and whether it is actually used in Spain to treat authorised symptoms. DESIGN: A descriptive, crossover and retrospective study. SETTING: Primary Care districts in the catchment area of the Virgen del Rocío hospital in Sevilla. PATIENTS: Those who requested permits for Ticlopidine prescriptions, documented by the clinical report. MEASUREMENTS AND MAIN RESULTS: The following data were recorded from the clinical reports presented when the Ticlopidine prescription permits were requested: patient's identification, diagnosis, pharmaceutical speciality, dosage, length of treatment and recommendation of haematological check-ups. Out of 407 reports available, the diagnosis corresponded to one of the authorised symptoms in 50.6% of cases. In a third of the patients the recommended dose was half that endorsed by the published clinical trials. Only in 2.7% of cases was mention made of the desirability of having haematological check-ups to forestall possible adverse reactions. The possibility of a counter-indication to Acetylsalicylic acid was only rarely mentioned. CONCLUSIONS: Ticlopidine is recommended in 50% of cases for symptoms for which it is not authorised in our country: moreover, in 35% of cases, at doses lower than those established as effective. The information given by the doctor who initiates the treatment is usually insufficient. There is a need to reassess whether cataloguing a medicine as "with hospital diagnosis" aids its rational use.

Aged↗