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M Casado

Publications and source records attributed to M Casado.

11 recordsLinked to original sources

L-glutamate transporter derived from mRNAs of primary glial cultures: expression in Xenopus laevis oocytes.

A high-affinity sodium-dependent L-glutamate transporter was expressed in Xenopus oocytes after microinjection of poly(A)+ RNA from primary astrocyte cultures from rat brain cortex. mRNA-induced L-glutamate transport was saturable by substrate and shows kinetic features similar to those found in intact glial cell preparations. L-Glutamate accumulation was prevented by rising the external K+ concentration or by coincubation with L-, D-aspartate or D-glutamate. After fractionation by sucrose density gradient, the mRNA encoding for the expressed L-glutamate transporter from glial cells was found in fractions containing messages of 2.05-2.9 kilobases (kb) in length.

Alkaline Phosphatase

Multiple linear cylindromas.

We report a case of multiple cylindromas with a linear arrangement on the lower right extremity. The patient had more than 100 tumors, but the scalp was spared. Some tumors showed overlapping features with eccrine spiradenoma. We believe this is the first report of multiple linear cylindromas.

Adenoma, Sweat Gland

[Prospective study of community-acquired pneumonias seen in a general hospital. Diagnostic errors].

BACKGROUND: The patients with community acquired pneumonias seen in an emergency service of a basic general hospital during one year were evaluated to assess their etiological, clinical and radiological features, and also to investigate the initial and final diagnosis of the disease, its evolution and the parameters associated with each microbiological type. METHODS: A medical team investigated daily the clinical records. For etiologic diagnosis, blood cultures, serological studies, urine counterimmunoelectrophoresis (CIE) and, in individualized patients, pleural fluid culture, bronchoaspirate and bronchoalveolar lavage were performed. The discordance between the initial clinical and radiological diagnosis and the microbiological results, and also the initial and final diagnostic errors were analyzed. RESULTS: 311 cases of pneumonia (150 adults and 161 children) were diagnosed. 95 (30%) had microbiological confirmation. Streptococcus pneumoniae was the most commonly isolated organism. Serologic studies were the diagnostic method with the highest yield. Complications developed in 28% of the patients and the mortality rate was 2%. There was a relationship between mortality and a high respiratory rate on admission. The initial-final diagnostic discrepancy was 43% in atypical pneumonias and 40% in bacterial pneumonias. CONCLUSIONS: Clinical parameters permitting the differentiation between atypical and bacterial pneumonia were not found. The initial diagnostic error was 12%, consisting of false positives in all instances, and the final diagnostic error included 15% false positives and 10% false negatives. Underlying diseases have a influence on the evolution of pneumonia. The mean respiratory rate on admission should be measured as a prognostic indicator. In the present study, urine CIE was a poorly sensitive method.

Adolescent

[Kaposi's sarcoma associated with chronic B lymphocytic leukemia. Coexistence of both diseases in skin lesions].

Kaposi's sarcoma is a rare malignancy. Its association with second primary cancers, especially of the lymphoreticular system, has been relatively frequently noted. We report a case of Kaposi's sarcoma (SK) in a 62 years-old women, affected by B-cell chronic lymphocytic leukemia (LLC B) for 22 month, that had received chemotherapy. Two skin lesions were seen on left lower limb. A biopsy revealed the coexistence of both diseases in the skin lesions. This finding is extremely rare. We review the possible etiopathogenic implications of this association and of the coexistence of both diseases in the same lesion.

Female

Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C.

The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), on high-affinity Na(+)-dependent gamma-aminobutyric acid (GABA) uptake were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with PMA led to concentration- and time-dependent decreases in the GABA transport in glial cells. This effect could be completely suppressed by addition of the PKC inhibitor H7. The PMA effects could be mimicked by oleoylacetylglycerol, the diacylglycerol kinase inhibitor R59022 and exogenous phospholipase C. Treatment with PMA did not affect GABA transport in neuronal cells.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine

Activation of high-affinity uptake of glutamate by phorbol esters in primary glial cell cultures.

The effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), a potent activator of protein kinase C, on high-affinity Na(+)-dependent glutamate transport were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with TPA led to concentration- and time-dependent increases in the glutamate transport that could be completely suppressed by the addition of the protein kinase C (PKC) inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine. The TPA effects could be mimicked by oleoylacetylglycerol and by the diacylglycerol kinase inhibitor R59022. The effects of TPA were potentiated by the Ca2+ ionophore A23187. Under the chosen experimental conditions TPA had no effect on glutamate transport in neurons. We conclude that PKC activates the sodium-dependent high-affinity glutamate transport in glial cells and that it has dissimilar effects on neurons and glial cells.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine

Acne naevus.

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Adult

Determination of monoamines in rat brain regions after chronic administration of lithium.

The effect of chronic administration of lithium on the concentration of biogenic amines and some of their metabolites in striatum, hippocampus, hypothalamus, pons-medulla and parietal cortex of rat were studied. Longterm lithium treatment modifies significantly the content of indoleamines in striatum and hypothalamus with minor changes in other structures. Catecholamine levels change after the treatment in striatum, hypothalamus, pons-medulla and parietal cortex. These results indicate that lithium treatment at therapeutic doses selectively modifies the catecholamine and indoleamine contents in discrete areas of the brain.

3,4-Dihydroxyphenylacetic Acid