Biomedical subjects
Publications and source records attributed to M Cerná.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
By means of sensitive and specific radiochromatographic methods the activities of adenosine deaminase and purine nucleoside phosphorylase in peripheral blood cells of patients with bronchogenic carcinoma were estimated. It has been shown that purine nucleoside phosphorylase activity was two- to three-fold higher in lymphocytes of patients with epidermoid as well as other types of bronchogenic carcinoma in comparison with a group of healthy individuals. Adenosine deaminase activity in lymphocytes was elevated only in the group of patient with nonepidermoid carcinoma. The authors suggest that especially the estimation of purine phosphorylase activity in lymphocytes would be a useful laboratory test in the study of immune responsiveness of patients with bronchogenic carcinoma.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
3-chloro-1,2-propanediol and 1,3-dichloro-2-propanol caused base substitutions in Salmonella typhimurium TA1535 both with and without metabolic activation. Metabolic activation seemed to act mainly by decreasing the toxicity of these compounds. A difference in the growth of the wild-type and repair-deficient strains of Escherichia coli was observed only for 1,3-dichloro-2-propanol with S9 mix. Esters of both chlorohydrines with fatty acids has smaller mutagenic effects than unesterified compounds.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The mutagenicity of 3-(5-nitro-2-furyl)acrylic acid (5-NFA) for Salmonella typhimurium tester strains TA100, TA98, TA1535 and TA1950 was studied by using the spot test, liquid-incubation test, microsomal assay, host-mediated assay and mutagenic analysis of blood and urine from treated mice. 5-NFA was dissolved in physiological saline or 10% ethanol. In the spot test, mutagenicity was found with TA100 and TA98 tester strains. A dose-related increase in mutation frequency was observed in the liquid-incubation test (at concentrations ranging from 1 to 50 micrograms 5-NFA/ml) with TA100 and TA1535; TA100 was the more sensitive. The use of ethanol reduced bacterial cell survival and increased the frequency of revertants. The Salmonella/microsomal assay showed no difference in mutagenic activity between the 5-NFA samples in tests with and without metabolic activation in vitro. In the host-mediated assay, mutagenicity of 5-NFA was detected after its applications in 3 fractionated doses. Subcutaneously applied 5-NFA was mutagenic after a total dose of 15 mg/kg, and after intragastric application of a total dose of 150 mg/kg. When blood samples from treated mice were analysed, the mutagenicity of 5-NFA at intragastric doses of 75, 150 and 300 mg/kg was observed for the tester strains TA100, TA98 and TA1950. The highest frequency of revertants was detected 15 or 30 min after administration of 5-NFA, the level being reached, according to the dose applied and strain used, after 1--2 h and confirmed in the course of 48 h. At the dose of 10 mg/kg, no mutagenic change was observed. Both tests on blood samples and host-mediated assay yielded identical results regardless of whether 5-NFA was dissolved in physiological saline or in 10% ethanol. Experiments in which mutagenic activity in urine was analysed demonstrated the mutagenicity of 5-NFA at the dose of 150 mg/kg with maxima after 24 and 48 h.
Human blood and urine mutagenicity of 3-(5-nitro-2-furyl)acrylic acid (5-NFA) was analysed by using Salmonella typhimurium indicator strains TA100 AND TA98 and the cytogenetic analysis of human peripheral lymphocytes. 8 human volunteers were given doses of 1 g 5-NFA per os. The mutagenic effect in blood was analysed after 0, 0.5, 1, 2 and 4 h, in urine after 0, 2 and 4 h. Cytogenetic analysis was done 0, 24 and 72 h after administration of 5-NFA. The experiment was repeated with 3 volunteers in the course of 96 h. When each of 8 volunteers consumed 1 g of 5-NFA, the mutagenicity was observed in 6 blood samples 1 h after exposure for strain TA98 (doubled number or revertants) and in all urine samples taken between the 2nd and 6th hours for both strains used. 7 volunteers given 10 mg 5-NFA in wine (2 sets) showed no mutagenicity of blood or urine for TA100 or TA98 indicator strains. These results are believed to indicate an enhanced elimination of 5-NFA from the human body.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.