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M Chevalier

Publications and source records attributed to M Chevalier.

32 records · Page 2Linked to original sources
PubMedDate not supplied

Determination of chloramphenicol residues by reverse phase high performance liquid chromatography in foies gras.

A reverse phase liquid chromatographic (LC) method for the determination of chloramphenicol (CAP) residues in foies gras was tested. The method was based on acetonitrile-sodium chloride extraction, sample clean-up with hexane and purification on a Sep-Pak C18 cartridge. Acceptable chromatographic separation was obtained for thawed samples with two sample clean-up steps with hexane, a purification step with acetonitrile + water (87.5 + 12.5) (v/v) before the elution of CAP residues by a mixture of acetonitrile + water (70 + 30) (v/v) with a chromatographic separation on Spherisorb ODS-2 (5 microns) column using sodium acetate buffer (pH 4.3) + acetonitrile (78 + 22) (v/v) as mobile phase. The detection limit of the method was 2.5 micrograms/kg. The mean recovery from spiked samples at the 10 micrograms/kg level was 55.7 +/- 8.7%.

Animals↗
PubMedDate not supplied

[Experimental study on the survival of Vibrio cholerae (El-Tor biotype) in sea-water].

Experimental studies on survival of Vibrio cholerae in untreated sea-water reveal that they remain alive at least one week. A somewhat longer survival (up to 3 weeks) could be observed if these bacteria were kept in sea-water sterilized by filtration. But if sea-water is autoclaved, cholera vibrios may keep their vitality for periods at least 6 months. Our observations lead to the conclusion that untreated sea-water may contains some filtrable thermolabile factor killing cholera vibrios. Yet these facts should not create false feelings of security. It is known, from recent epidemiological data that sea-medium as a whole may be the origin of cholera outbreak.

Seawater↗