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M Chicurel

Publications and source records attributed to M Chicurel.

15 recordsLinked to original sources
PubMed2000

Virology. Probing HIV's elusive activities within the host cell.

Once inside a host cell, HIV must make its way to the nucleus in order to replicate, but until now, its journey has largely been uncharted territory. As researchers begin to explore this landscape a common theme is emerging: Soon after viruses enter cells, they enlist components of their hosts to assist them in their journey. Now, in what is being called one of the most exciting frontiers in AIDS research, preliminary findings on how HIV enters cell nuclei are giving AIDS researchers new leads on possible drug targets to fight the ever-mounting epidemic.

Cell Membrane↗
PubMed2000

Cell biology. Color-changing protein times gene activity.

For years, researchers trying to capture the bustling activity of genes in living cells have mostly had to make do with snapshots, which clearly fell short of the mark. But now their frustration may be over. On page 1585, a team describes a new fluorescent protein that turns bright green when it is first made, then changes to red over several hours--providing the ability to witness how genes alter their activities over time.

Animals↗
PubMed1988

Modulation of macrophage lysosomal pH by Mycobacterium tuberculosis-derived proteins.

Macrophage lysosomal pH was significantly (greater than 1 pH unit) increased in a reversible, concentration-dependent manner characterized by a saturable and cyclic kinetics after exposure to culture filtrate protein extract derived from Mycobacterium tuberculosis. Lysosomal alkalinization peaked 30 min after administration of culture filtrate protein extract to cells of the macrophagelike cell line J774A.1. The alkalinization was reversible, and a second peak was observed approximately 60 min after incubation. Maximum lysosomal alkalinization increased as a function of culture filtrate protein extract concentration, reaching an apparent saturation level around 700 to 1,000 micrograms/ml, although the time course for this process was not significantly dependent on antigen concentration. The alkalinizing agent(s) was heat labile and produced a similar effect in cells which had a different lysosomal enzyme composition. Our observations are consistent with the presence of one or more mycobacterial antigens which have a pH-dependent affinity for lysosomal structures essential for lysosomal acidification and which are able to inhibit this lysosomal acidification.

Animals↗
PubMedDate not supplied

[Presentation of salmonellosis in the Tandil district, Argentina].

From January 1990 to July 1992 a prospective study on the salmonellosis form of presentation, reservoirs and transmission in Tandil was carried out. Forty strains of Salmonella enterica were isolated with the following distribution: 28 humans (17 children and 11 adults), 6 in foods (1 in raw milk, 3 in well water, 1 in home made mayonnaise and 1 in chicken salad) and 6 in animals (1 in cow, 2 in chicken and 2 in calves). Salmonella Enteritidis was the most frequent isolate. Disease was evident only in a few susceptible hosts and the epidemiological chain could not be determined in all the cases.

Adult↗