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Biomedical subjects

M Cobeljić

Publications and source records attributed to M Cobeljić.

At least 19 recordsLinked to original sources
PubMed1996

Enteroaggregative Escherichia coli associated with an outbreak of diarrhoea in a neonatal nursery ward.

Over a 9-day period in February 1995, 16 newborn babies (age range 2-11 days) and 3 infants (24, 47 and 180 days of age) in a neonatal nursery ward developed diarrhoea accompanied by pyrexia and weight loss. Known enteropathogens were not detected in their stools but Escherichia coli displaying aggregative adherence to HEp-2 cells (enteroaggregative E. coli) were found in 12 (63%) ill infants and in none of 5 well neonates (P = 0.02). The illness lasted 3-9 days (mean 5.2) in 16 babies, whereas in 3 neonates it showed a protracted course of 18-20 days. The source of infection and the mode of transmission remained unclear. The outbreak isolates manifested properties common in this new group of diarrhoeagenic E. coli: mannose-resistant haemagglutination, haemolysis on blood agar, and clump formation in liquid culture medium. They belonged to the O4 E. coli serogroup and expressed multiple antibiotic resistance.

Bacterial Adhesion↗
PubMed1995

Infrequent finding of verotoxin-producing Escherichia coli in diarrheal stools in Belgrade, Serbia.

Out of 2,638 patients, with acute diarrhea, verotoxin-producing Escherichia coli (VTEC) was isolated from the stools of 8 (0.3%). There was no visible blood in their stools and none of them developed other syndromes associated with VTEC infection. None of the isolated VTEC agglutinated with O157 E. coli antiserum, while only 3 strains hybridized with the DNA probe CVD 419, designed to detect VTEC. The data obtained suggest that VTEC is not an important causative agent of acute diarrhea in Belgrade.

Acute Disease↗
PubMed1991

The ability of rifampin-resistant Escherichia coli to colonize the mouse intestine is enhanced by the presence of a plasmid-encoded aerobactin-iron(III) uptake system.

Rifampin-resistant Escherichia coli are known to be poor colonizers of the animal intestine. In this report, we show that the colonizing ability of rifampin-resistant E. coli cells is increased dramatically in the presence of the aerobactin-mediated iron(III) uptake system. In contrast, the colonization by nalidixic acid-resistant E. coli does neither depend on the aerobactin-iron(III) nor on the dicitrate-iron(III) uptake system. Likewise, it does not depend on the production of the siderophore enterochelin.

Animals↗
PubMed1991

Haemolytic-uraemic syndrome associated with Aeromonas hydrophila enterocolitis.

Haemolytic-uraemic syndrome (HUS) associated with Aeromonas hydrophila enterocolitis is reported in a 23-month-old female infant. The A. hydrophila strain isolated from the patient's stool sample produced cytotoxin against verocells; increasing levels of cytotoxin-neutralizing antibody in the patient's sera were demonstrated, suggesting a recent infection. This report indicates that A. hydrophila should be suspected as a possible cause of HUS, and that this pathogen should be looked for in cases of post-diarrhoeal HUS.

Aeromonas↗
PubMed1990

Lack of association of Escherichia coli exhibiting both mannose-resistant haemagglutination and diffuse adherence to HEp-2 cells with acute diarrhoea in children.

Stool specimens from 631 children with acute diarrhoea and from 277 healthy controls were tested for the presence of non-enteropathogenic, non-enterotoxigenic Escherichia coli strains which mediated mannose-resistant haemagglutination of human erythrocytes (MRHA+). Fifty-nine (34.9%) of 169 isolated MRHA+ strains but none of 210 MRHA- strains exhibited diffuse adherence (DA+) to HEp-2 cells. DA+ strains were found in 37 (5.9%) children with diarrhoea and in 22 (7.9%) controls. MRHA+/DA+ strains in comparison to MRHA+/DA- strains significantly less frequently expressed P fimbriae (10.7 vs. 73.6%), haemolysin production (12.5 vs. 63.2%), and MRHA of other species erythrocytes (21.4 vs. 84%). These data demonstrate that E. coli which exhibit the diffuse pattern of adherence to HEp-2 cells also cause MRHA of human erythrocytes. Since these strains were found with similar frequencies in children with and without diarrhoea it seems that DA is not a marker of enteropathogenicity of E. coli.

Acute Disease↗
PubMed1989

The association of enterotoxigenic and enteropathogenic Escherichia coli and other enteric pathogens with childhood diarrhoea in Yugoslavia.

The presence of enterotoxigenic and enteropathogenic Escherichia coli (ETEC and EPEC, respectively) was investigated in stool specimens of 1082 preschool children with diarrhoea and in stools of 335 healthy controls in localities in southern Yugoslavia, as well as in 566 children with diarrhoea and in 231 controls living in northern part of the country, during the seasonal peak (August-November) of enteric diseases in 1986. ETEC were found in 114 (10.5%) children with diarrhoea and in 14 (4.2%) controls (P less than 0.001) in the southern part, and in 26 (4.6%) ill children and one (0.4%) well child (P less than 0.005) in the northern part of Yugoslavia. EPEC were isolated from stools of 85 (7.9%) children with diarrhoea and of 14 (4.2%) well children (P less than 0.05) in localities of southern Yugoslavia, and from 22 (3.9%) ill children and from 10 (4.3%) controls in northern Yugoslavia. Nineteen EPEC strains expressed localized adherence to HEp-2 tissue culture cells; all were isolated from stools of ill children. In southern Yugoslavia, where other enteropathogens were sought, the most commonly found agents in ill children were shigellae (17.5%), rotavirus (11.8%), ETEC, and EPEC. Potential pathogens were detected in 44.5% cases of sporadic diarrhoea and in 15.8% controls. This study revealed that ETEC were associated with acute diarrhoeal disease in Yugoslav preschool children. On the other hand, the diagnosis of EPEC-diarrhoea by routine determination of serogroup established the association of these agents with sporadic diarrhoea only in the 0-2 years age categories in all investigated localities. In the less developed southern part of Yugoslavia bacteria were the predominant causative agents of enteric illness during the seasonal peak of this disease.

Acute Disease↗
PubMedDate not supplied

[Isolation of Shigella boydii serotype 14 with altered biochemical characteristics in a water-borne epidemic of dysentery].

Two water-borne epidemics of bacillary dysentery have been described in a garrison in which the leading causative agent has been shigella boydii, serotype of 14 altered biochemical characteristics isolated for the first time in Yugoslavia. All the isolated strains of this shigella were mannitol negative and they degradated gylocose to acidity and gas already after 24 hours, and arabinose and sorbitol to acidity and gas after 48 hours. The clinical picture of the diseased was characteristic of bacillary dysentery and did not differ from clinical picture of patients in whom other serotypes of shigella were isolated.

Disease Outbreaks↗