PubMed Health⌕ Search

Biomedical subjects

M Coletta

Publications and source records attributed to M Coletta.

135 records · Page 8Linked to original sources
PubMedDate not supplied

Correlation between osteoarthritic cartilage damage and levels of proteinases and proteinase inhibitors in synovial fluid from the knee joint.

Matrix metalloproteinases (MMPs) in the synovial fluid are responsible for collagen breakdown during physiologic cartilage turnover and the pathologic destruction of the cartilage. We measured the levels of MMPs, specific tissue inhibitors of metalloproteinases (TIMPs), and interleukin-6 (IL-6) in synovial fluid from the knees of 36 patients with cartilage lesions subdivided according to severity based on arthroscopic findings. Lesions were classified as mild (group 1, edema with no disruption of the surface), moderate (group 2, open lesions without exposure of subchondral bone), or severe (group 3, exposure of subchondral bone). Zymography (gel electrophoresis in the presence of hydrolizable substrates) showed a 60-kd band in all samples. A second band (94-kd) was found exclusively in specimens from groups 2 and 3, and a third band (110-kd) was present only in group 3. Concentrations of 2 of the most important modulators of MMP activity, TIMP-1 and IL-6, were measured. TIMP-1 levels did not vary significantly with the severity of cartilage damage. Linear regression analysis revealed a significant positive correlation between TIMP-1 and IL-6 in groups 1 and 2. These data indicate that the severity of the cartilage damage corresponds with MMP activity. The correlation between IL-6 and TIMP-1 in groups with mild and moderate damage suggests a regulating mechanism that is absent in severe lesions.

Adult↗
PubMedDate not supplied

[Hirudin inhibition of human thrombin activity].

Thermodynamics and kinetics for hirudin binding to human alpha-, beta- and gamma-thrombin, under experimental conditions which mimic the in vivo conditions (i.e., pH = 7.35, T = 37.0 degrees C, I = 0.1 M), indicate that the inhibitor specificity for the three species of the enzyme is different. Such a finding agrees with the human thrombin:hirudin binding geometry as revealed by X-ray crystal studies. From the therapeutic viewpoint, thermodynamics and kinetics here reported indicate that the inhibitory activity of hirudin in vivo is more effective than that shown by antithrombin III, which is generally considered the most important plasma thrombin inhibitor.

Hirudins↗
PubMedDate not supplied

[Low levels of somatomedin C and short stature in the Silver-Russell syndrome].

The authors describe one case of Silver-Russell syndrome, emphasized the etiopathogenetic aspects connected to it. As regards the short stature, they have proved that the syndrome is related to low levels of somatomedin C (SmC). Moreover, they emphasized that ambiguous genitalia gets more and more frequently a peculiar features of syndrome.

Body Height↗
PubMedDate not supplied

[Case report of asymptomatic toxocariasis. A 24-month follow up].

An asympthomatic case of toxocariasis is described in a 9 years boy, who had been under follow-up observation for 24 months. He showed only a marked eosinophilia (42%) without any clinical alterations. The IgG antitoxocara dosage, performed by immunoenzymatic method, allowed to formulate the diagnosis. The oculistic checks, together with EEG carried out the time of diagnosis as well as at the end of follow-up did not show any alterations. No therapy was practised and the number of eosinophilis in to circulation went progressively down. At the end of follow-up the percentage of eosinophilis is 7%.

Animals↗
PubMedDate not supplied

[Variations in renal hemodynamics during acute renal insufficiency in anoxic neonates].

The purpose of this study is to verify if the increase of renal resistance, along with the consequent reduction of glomerular filtrate, and the activation of renina-angiotensin system, could be attributed to not only neonatal acute hypoxia but to other factors as well. These factors could provoke an increase in renal vascular resistance (R.V.R.), a reduction of renal blood flow (R.B.F.) and renal glomerular filtrate (R.G.F.) condition. These components are present in angiotensin as well as in endothelin (ET1), a potent peptide from vascular endothel with vasoconstricting action and whose secretion increases during hypoxia. The Authors have studied and compared two groups of newborns. The first group of newborns was affected by perinatal asphyxia or hypoxia of variable seriousness. It included 24 newborns with gestational age between 37 and 41 weeks and with a birth weight between 3.200 gms and 3.500 gms. The second, control group, was made of an identical number of newborns of the same gestational age with a weight adequate to the birth age. The dosage of the ET1 was evaluated on the plasma, using the RIA method. The diagnostic criteria for this evaluation included clinical and biohumoral evidence (tab. 1). Between the two groups, group 1 with anoxic syndrome showed the more intense increase of ET1. This increased is due to either an increase value of plasmatic creatinine that is present in normal situations or in physiopathologic role played by the ET1 in course of acute renal failure after asphyxia.

Acute Kidney Injury↗
PubMedDate not supplied

[Parasitic arthritis: a case report].

In Protozoan-type infections muscular and/or articular manifestations are not unusual. They are directly correlated to the presence of the parasite in the organism. In such cases a good diagnostic support can be given by the demonstration of the parasitic infection, the inefficacy of the classical therapy with FANS or steroids, and the good results obtained with a specific antiparasitic therapy. The most significant data that emerge from this description regard the possibility that when an arthritis or an arthralgia cannot be defined a parasitic infection has also to be excluded.

Animals↗