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M Corrales

Publications and source records attributed to M Corrales.

17 recordsLinked to original sources
PubMed1990

Hyaluronic acid (Sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens.

The effects of Sperm Select (Pharmacia AB, Uppsala, Sweden), a hyaluronic acid medium, on the motility and membrane integrity properties of sperm were studied. In 15 normospermic specimens after overnight incubation, the motility parameters in the control versus the Sperm Select group were as follows (mean +/- SEM): motility, 18.8% +/- 2.8% versus 27.4% +/- 2.9%; velocity, 21.5 +/- 2.4 versus 27.2 +/- 2.2 microns/s; linearity, 3.8 +/- 0.3 versus 4.4 +/- 0.2; lateral head displacement, 1.5 +/- 0.2 versus 1.9 +/- 0.1 microns; and tail beat/cross frequency, 8.8 +/- 1.3 versus 10.8 +/- 1.4 Hz. The density of motile sperm was 10.8 +/- 2.3 versus 18.5 +/- 2.5 X 10(6) sperm/mL. Finally, the velocity coefficient, the multiple of the sperm motility and linear velocity, was 4.6 +/- 1.1 versus 8.1 +/- 1.4. However, we found no Sperm Select related differences when testing sperm membrane integrity with hypoosmotic swelling and supravital staining. Thus, Sperm Select improves the retention of sperm motility (most prominently velocity) apparently due to a direct action of hyaluronic acid on sperm metabolism or contractility rather than to preservation of sperm membrane integrity. In 20 oligospermic specimens, Sperm Select caused similar improvements in sperm motility, and the duration of motility could be predicted from the degree of enhancement in sperm velocity after short-term Sperm Select exposure. A modified Sperm Select protocol is described that further increases motile sperm yield without a centrifugation step.

Bisbenzimidazole↗
PubMed1988

Correlation between sperm creatine phosphokinase activity and sperm concentrations in normospermic and oligospermic men.

Toward the development of biochemical probes for the assessment of sperm function we have measured the activities of sperm creatine-N-phosphotransferase (CPK). There was a highly significant inverse correlation (P less than 0.001 in all comparisons) between sperm CPK activities and sperm concentrations in specimens of normospermic and oligospermic men with greater than 30 million sperm/ml (0.106 +/- 0.01 SEM, N = 90, expressed as CPK U/100 million sperm), 20-30 million sperm/ml (0.333 +/- 0.07 SEM, N = 30) and 10-20 million sperm/ml (0.583 +/- 0.12 SEM, N = 30) when compared with the CPK values of the less than 10 million/ml specimens (2.242 +/- 0.46 SEM, N = 30). Furthermore, the distribution of CPK activities within these four groups showed that 96%, 67%, 43%, and 4% of the samples, respectively, were in the less than 0.250 CPK U/100 million sperm normal range (mean + 2 SD of the greater than 30 million sperm/ml group). However, there was no relationship between sperm CPK activities and the values of sperm motility (P greater than 0.15) or morphology (P = 0.38) in the samples. The migrated sperm fractions (significantly improved in motility and velocity parameters) showed CPK activities lower than the initial semen specimens (P less than 0.01, N = 150). In fact, in some oligospermic men the CPK activities of the migrated sperm fractions were within the range of normospermic samples.(ABSTRACT TRUNCATED AT 250 WORDS)

Creatine Kinase↗
PubMed1988

Sperm creatine phosphokinase activity as a measure of sperm quality in normospermic, variablespermic, and oligospermic men.

We have found a significant inverse correlation between sperm concentrations and sperm creatine N-phosphotransferase (CPK) activities in oligospermic and normospermic human specimens. In the present work, we carried out serial CPK determinations to assess whether there is a relationship between fluctuating sperm concentrations and sperm quality in consistently oligospermic and variablespermic (sperm concentrations are occasionally in the greater than 20 million/ml range) husbands of 65 couples (23 normospermic men/51 samples, 25 consistently oligospermic men/80 samples, and 17 variablespermic men/68 samples). The sperm CPK activities were significantly lower in the normospermic vs. the oligospermic or variablespermic groups (p less than 0.001), but there were no differences between the latter two (p greater than 0.25). The mean CPK values of migrated sperm fractions in both the oligospermic and variablespermic populations were improved (at least 20% decline in CPK values) compared to those of the initial specimens (1.27 +/- 0.38 vs. 0.68 +/- 0.37 and 0.77 +/- 0.32 vs. 0.46 +/- 0.24 SEM U/100 million sperm, respectively, p less than 0.001 in both pairs) and the incidence of the "failed-to-improve" samples was also similar in the two groups (44/36 vs. 45/23, p greater than 0.2). The lack of differences in the mean CPK activities, in the distribution of CPK values under and over 0.250 U/100 million sperm level, and in the ratio of migrated samples with improved or with failed-to-improve CPK activities suggests that sperm quality is not different between men who are consistently oligospermic and those who occasionally produce normospermic specimens.(ABSTRACT TRUNCATED AT 250 WORDS)

Creatine Kinase↗
PubMed1976

The third ventricle. Normal anatomy and changes in some pathological conditions.

An anatomical study of the normal anterior inferior part and the floor of the third ventricle has been made and its radiological appearance shown. Specimens and casts were analyzed and correlated with ventriculograms using watersoluble contrast media. The detailed appearance of the optic recess and sulcus of Monro, which has not been well described in the neuroradiological literature, is illustrated and detailed. The changes in the optic recess in hydrocephalus are discussed. Deformities of the different segments of the floor as a result of tumors of the posterior fossa and hydrocephalus are shown.

Cerebral Ventricle Neoplasms↗
PubMed1975

The chain guide technique for selective ventriculography.

A technique for selective ventriculography using a chain as a guide, utilizing gravity has been previously described by the author. Additional details of manipulation and injections are now reported. The cisterns have also been reached with the technique and narrow spaces as aqueductal stenosis has been passed through with the chain.

Cerebral Ventriculography↗
PubMedDate not supplied

Sperm creatine kinase activity in fertile and infertile oligospermic men.

The authors examined the value of sperm creatine-kinase (CK) activity parameters to predict sperm fertilizing potential of oligospermic men. Two patient groups from our intrauterine insemination program were studied: fertile oligospermic (32 men/46 specimens) and infertile oligospermic (19 men/82 specimens). In the initial specimens, the CK activities were (mean + SEM IU CK/10(8) sperm): 0.53 +/- 0.09 and 1.17 +/- 0.19 (P less than 0.001). The corresponding values in the swim-up fractions were 0.32 +/- 0.06 and 0.67 +/- 0.08 (P less than 0.001). In a subset of samples by fertile (N = 33) and infertile (N = 66) oligospermic men who had close to identical sperm concentrations (11.9 +/- 0.9 vs. 11.9 +/- 0.5 million sperm/ml) and motility values (23.7 +/- 1.7 vs. 23.0 +/- 1.3%), the CK activities were significantly lower in the fertile group in both the initial (P = 0.02) and in the swim-up (P = 0.002) samples. A logistic regression analysis of all 160 study samples (including 21 normal men/32 samples) further demonstrated that CK activities were predictive of fertilizing potential, whereas sperm concentrations of the samples provided no additional contribution. Sperm CK and similar biochemical markers will facilitate selection of men for various approaches in assisted reproduction.

Creatine Kinase↗