Microtiter plate assay for sexual agglutination in the yeast Hansenula wingei.
A fast method is described for determining the mating type of yeast spore clones independent of genetic complementation between auxotrophic markers.
Biomedical subjects
Publications and source records attributed to M Crandall.
A fast method is described for determining the mating type of yeast spore clones independent of genetic complementation between auxotrophic markers.
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Late reactions consisting of fever, malaise, and swelling at the site, 4 to 6 hr after injections of Alternaria extract occurred in several patients receiving immunotherapy with Alternaria. These patients had in common serum IgG precipitins and exquisite leukocyte histamine release sensitivity to Alternaria. Such precipitins were 3 times more frequent in patients receiving Alternaria immunotherapy than a control group of patients receiving immunotherapy with other antigens. A prospective study revealed that 5 of 23 Alternaria-sensitive persons had precipitins before immunotherapy and another 6 developed precipitins during therapy. Only one of the 23 experienced a late Alternaria reaction. Thus, precipitins to Alternaria are common and do not seem to be the basis for the late reactions we observed. The finding of precipitins does not contraindicate immunotherapy.
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