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M Cresti

Publications and source records attributed to M Cresti.

24 records · Page 2Linked to original sources

The SH-SS exchange reaction between the Ellman's reagent and protein containing SH groups as a method for determining conformational states: tubulin.

Reactivity of tubulin SH groups, estimated by the slope to the curve in the SH-SS exchange reaction with 5-5' dithiobis (2-nitrobenzoic acid), was compared with that of bovine serum albumin (BSA) and studied in presence of various ligands. A small part of tubulin SH groups (12%) showed a higher reactivity, while the remaining portion had a smaller reactivity than that of BSA. The SH group reactivity of tubulin but not the total amount (12.8 mu/mole) diminished when assayed with colchicine and MgCl2 (0.1 and 0.2 mM, respectively); 0.2 mM CaCl2 increased the reactivity at the maximum level. On the basis of the biological role of tubulin SH groups and of the opposite effects exerted by Ca++ and Mg++ on the protein, the results presented here seem to indicate a correlation between conformational states of tubulin and its biological functions.

Animals↗

The pollen calendar of Siena (Central Italy).

The atmospheric pollen count at Siena was carried out for 4 years (1978-1981) to determine the average quantity of airborn pollen and the families, genera or species which contribute to such pollen dispersal. The pollination period stretches from February to mid October. The highest pollination period is from May to July when the most important pollen producers are Poaceae, Urticaceae, Pinus, Quercus, Oleaceae and Castanea sativa. Also in March there are high pollen production values due to Cupressaceae, Corylaceae, Alnus and Ulmus.

Italy↗