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M Crone

Publications and source records attributed to M Crone.

At least 37 records · Page 2Linked to original sources
PubMed1982

Specificities of anti-rabbit VH antibodies in mammalian and fowl antisera.

Antibodies against rabbit VH-determinants were produced by immunizing mammals and birds with rabbit IgG, IgA or VH. Most of the anti-VH produced in mammals was specific for allotype VH-determinants, but small amounts of antibody against non-allotype VH-determinants were also produced. By contrast the immunization of chickens led to the production of predominantly non-allotype VH antibody. Further analysis of the antibody specificity revealed the production of antibody against VH-determinants available in the intact immunoglobulin (H- + L-chain) as well as antibody reacting with determinants only accessible in the absence of L-chain. Antibody of the latter specificity prevailed after immunization with VH. The purified VH was not deficient in antigen determinants when compared with VH in the intact immunoglobulin (IgA). Unexpectedly, none of our chicken anti-rabbit VH antisera showed crossreactivity with VH from other mammalian species.

Animals↗
PubMed1982

The major histocompatibility complex of outbred chickens. I. Analysis of the B13 haplotype by serology and cellular reactions.

The F antigens, which are serologically defined Class I gene products of the major histocompatibility complex in chickens (the B complex), were analysed in outbred birds. Private specificities of the F13 antigen from the inbred CC strain were detected in 20 outbred chickens by a haemagglutination technique. In the GVH-inhibition-release test F13 antigens from outbred and inbred chickens were identical. The L antigens, which are the Class II antigens of the B complex, were detected with specific anti L13 alloantisera by indirect immunofluorescence. Antisera defining the L13 antigen(s) of the inbred CC line reacted with all F13 positive outbreds. As a test of one-way direct compatibility of the inbred and outbred animals typing F13, graft versus host reactions were performed, injecting blood of F13 positive outbreds into inbred B13/13 eggs. No GVH stimulation attributable to MHC determinants was found. In MLR, responder cells from outbred MHC heterozygous chickens, which typed F13/x, were stimulated by inbred F13/13 homozygous typing cells, and weak, but significant, reactions were found. Further analysis in the accompanying paper, however, revealed no difference in the lymphocyte activating determinants (Lads) of inbred and outbred birds typing F13 and L13. No individual has yet been found which carries one of these antigens in the absence of the other.

Animals↗
PubMed1981

[Results of an open study and a controlled comparative study of feprazone and naproxen in patients with rheumatoid arthritis or osteoarthrosis (author's transl)].

The so-called non-steroid antirheumatics have considerable significance in medical armamentarium for controlling polyetiological complexes of diseases. The numerous new developments are designed to reduce the side effects, some of which seem inevitable with increased efficacy. In this two-part study the authors deal with the new Feprazone. Prior to a double-blind randomized comparative study against Naproxen, an open study on effect and tolerance was performed in ten rheumatic patients over three months. In the second part a controlled study was carried out in 30 patients for four weeks following this treatment. The patients half of whom were each given 600 mg Feprazone or 750 mg Naproxen showed a definite improvement in the rheumatic symptoms as compared with their initial situation. A difference in therapeutic efficacy and tolerance was only noticeable in a slight trend in favour of Feprazone.

Adult↗
PubMed1981

Evidence for two populations of B-L (Ia-like) molecules encoded by the chicken MHC.

Two specific alloantisera detecting B-L (Ia-like) antigens on chicken lymphocytes of the B6 and B15 haplotypes were found to cross-react strongly. Anti-B-L6 and anti-B-L15 alloantisera both reacted with B-L molecules on B6 and B15 lymphocytes as demonstrated by immunofluorescence and SDS-PAGE analysis of 125I-labeled B-L antigens isolated by incubation with anti-B-L alloantisera. Absorption studies showed that the anti-B-L alloantisera reacted with at least two kinds of antigenic determinant, one set shared by B-L6 and B-L15 molecules and another set specific for each haplotype. In spite of the absence of genetic evidence for more than one B-L locus in the chicken B complex, it was shown by sequential antibody incubations that these two different B-L antigenic determinants are associated with at least two separate species of B-L molecules, indicating the presence of at least two B-L loci within the MHC of the chicken.

Animals↗
PubMed1981

Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG.

Eggs from immunized chickens may provide a convenient and inexpensive source of antibodies. We describe two simple and efficient methods for purification of IgG from yolk. The antibody is rendered useful for most currently employed immunological techniques. Amounts of antibody corresponding to almost half a litre of antiserum may be recovered from a chicken in one month.

Animals↗
PubMed1981

B-L antigens (Ia-like antigens) of the chicken major histocompatibility complex.

This paper reviews the present knowledge of B-L antigens encoded by the chicken B complex as regards to the following aspects: (1) identification and cellular expression, (2) structural studies, (3) evidence for two distinct populations of B-L antigens, (4) mapping of B-L loci of the B complex, (5) B-L and immune response, and (6) the role of the B-L antigens for the control of mixed lymphocyte reactions (MLR) and graft-versus-host (GVH) reactions. It is concluded that B-L antigens of the chicken exhibit extensive homology with mammalian Ia antigens. A genetic map of the B complex is presented.

Animals↗
PubMed1981

The still elusive T cell receptor: on the possibility of a common V-gene pool for B- and T-cell-antigen receptor molecules.

The contention that VH constitutes a part of T-cell receptors for antigens was probed by purifying rabbit T cells and analysing these cells for non-immunoglobulin VH, i.e. VH not associated with L chain. A number of anti-VH antisera were employed for this purpose, the most important being goat antiserum, reacting with common a1 allotype determinants (allotype determinants expressed on free VH and H chain as well as on intact immunoglobulins), rat antibody against common non-allotype VH determinants (VH framework determinants expressed on VH and H chain as well as on intact immunoglobulins) and chicken antibody against unmasked non-allotype determinants (VH determinants accessible only in the absence of L chain). VH and L chain was quantified by radioimmunoassays on extracts and supernatants from unstimulated T cells as well as from T cells stimulated by concanavalin A and by allogeneic cells. Absolute depletion of Ig-containing and -producing cells was not achieved but in no case was an excess of VH over L chain observed. This indicated that all detected VH originated from cells of the B lineage. The cells were also cultured in the presence of labelled amino acids followed by analysis of detergent extracts and supernatants by immunoadsorption and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) fluorography. Again, no evidence for T-cell VH could be found. Affinity purified anit-VH antibody was used to label viable rabbit T cells through the use of secondary fluorescence-labelled anti-immunoglobulin antibody. No VH-specific labelling of T cells could be observed. Mixed lymphocyte cultures were carried out in the presence of affinity-purified anti-VH antibodies. No inhibition of the reaction could be discerned. The failure to detect T-cell VH is in agreement with the recent finding that the VH-genome in T cells is not rearranged in a functional manner similar to that in B cells.

Animals↗
PubMed1977

Interaction between a synthetic polypeptide, TIGAL, and Fc receptors on non-bursa-derived chicken lymphoid cells.

Autoradiographic studies have shown that radioiodinated TIGAL binds in vitro to a small but varying fraction of lymphoid cells from bursectomized, agammaglobulinemic chickens, whereas no binding of radioiodinated TGAL or a variety of other radioiodinated antigens can be observed. The binding of [125I]TIGAL is inhibited by antigen-antibody complexes. Radioiodinated antigen-antibody complexes are bound to a similar proportion of the lymphoid cells from bursectomized chickens, and this binding is inhibited by preincubation of the cells with unlabeled TIGAL but not with TGAL. These results indicate a cross-reaction at the level of Fc receptors between determinants on TIGAL and on IgG.

Albumins↗
PubMed1976

Allo-aggression in chickens. II. Cellular expression of the AA gene products.

B1-anti-B2 and B2-anti-B1 allo-antisera were raised by i.v. immunization of homozygous B1/1 and B2/2 chickens, respectively, with B1/2 cells of the following sources: peripheral blood leucocytes (PBL), erythrocytes, thymus cells and bursa cells. Purified suspensions of these all produced anti-B allo-antibodies which could both haemagglutinate red blood cells and strongly inhibit the GVH reactivity of blood lymphocytes of the original donor type. Absorption studies of the sera using cells of the same four sources showed that they could all completely remove the GVH inhibitory antibodies. A fact of particular importance is that B-haplotype determined antigens which are common to erythrocytes and lymphocytes seem to include the gene products of the allo-aggression locus, as earlier defined (Simonsen 1975).

Animals↗
PubMed1975

Antigen binding by non-bursa-derived chicken leukocytes.

Antigen-binding peripheral blood leukocytes (PBL) from normal and bursectomized agammaglobulinemic chickens were labeled by incubation in vitro with radioiodinated antigen at 4 degrees C in the presence of sodium azide. [125I]TGAL-binding cells could be detected by autoradiography of PBL from normal, unimmunized chickens at a frequency of 1 to 4 labeled cells per 10(4) leukocytes. No [125I]TGAL-binding cells were found in PBL from bursectomized chickens, even after incubation with 25 mug/ml of labeled antigen followed by prolonged autoradiographic exposure. The binding to normal PBL was specific as judged by inhibition with unlabeled TGAL but not with unlabeled TIGAL. The binding was, furthermore, inhibited by preincubation with rabbit anti-chicken L chain antibody but unaffected by normal rabbit IgG. [125I]TIGAL was, in contrast, found to bind to PBL from both normal and bursectomized chickens at a frequency of 6 to 80 labeled cells per 10(4) leukocytes. The labeling was specific, since it was inhibited by cold TIGAL but not by cold TGAL. The binding of [125I]TIGAL to PBL from bursectomized chickens showed from none to slight inhibition on preincubation of the cells with anti-L chain antibody, whereas preincubation with normal rabbit IgG resulted in almost complete inhibition. To our knowledge this is the first demonstration of antigen binding to PBL from agammaglobulinemic chickens.

Agammaglobulinemia↗