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Biomedical subjects

M Csik

Publications and source records attributed to M Csik.

13 recordsLinked to original sources
PubMed1990

Mutagenicity of emission and immission samples around industrial areas.

Urban air particulates (suspended particles and settling dusts), as well as dusts emitted by a Söderberg aluminium production plant and a coal-burning power plant, in the industrial town of Ajka were analysed for polycyclic aromatic hydrocarbon (PAH) content (liquid chromatography) and mutagenicity (Salmonella microsome test). The same parameters for air particulates from Pápa (a town of similar size without much heavy industry) were analysed and compared to those from Ajka. The dust content, PAH concentration and the mutagenic potency of the air in Ajka were higher than in Pápa. Mutagenicity of airborne particulates showed a clear seasonality with a winter maximum and a summer minimum in both towns. The mutagenic potency of air correlated well with the benzo[a]pyrene (BP) and PAH content in Ajka but not in Pápa. The amounts of extractable organic material and mutagenic potency, as calculated for unit quantity of airborne particulate matter, were higher in the Pápa samples. Similar differences between the two towns were observed in the case of deposited dust. As regards the emission dusts, the aluminium plant emissions are responsible for much more of the mutagenicity of the urban air than the power plant emission, which is the main component of air dust pollution in Ajka.

Air Pollutants↗
PubMed1989

Genotoxicity of selected herbicides.

Twenty-two herbicides were studied in 67 tests for induction of DNA damage, gene mutation and chromosomal changes in vitro and in vivo. Triazine and urea-type herbicides were found to be inactive in all but one test. Of 4 thiocarbamates, molinate and vernolate caused chromosomal changes, namely increased incidence of sister chromatid exchanges and chromosomal aberrations in vitro and increased frequency of micronucleated polychromatic erythrocytes in mouse bone marrow. These compounds, however, did not cause gene mutation and only molinate gave equivocal positive result in bacterial repair test. Out of 11 miscellaneous herbicides, ethofumesate, alachlor, dichlorprop and fluorodifen proved to be positive only in one or two tests. In the light of clastogenicity of some thiocarbamates, serious consideration should be given to start animal carcinogenicity studies with these chemicals.

Animals↗
PubMed1989

Mutagenicity and PAH content of airborn particulates and of fallen dusts from two Hungarian towns and emission samples from aluminum reduction and power plants.

Urban air particulates (suspended particles and settling dust), furthermore dust emitted by a Soderberg aluminum reduction plant and a coal burning power plant from an industrial town, Ajka (30,000 inhabitants) were analysed for PAH content (liquid chromatography) and mutagenicity (Salmonella microsome test). Air particulates from Papa--a town of similar size without considerable heavy industry--and corresponding plant emission from Inota, a third town in the study, served as controls. The dust content and the PAH concentration, as well as the mutagenic potency of the air in Ajka were higher than in Papa. Mutagenicity of the airborne particulates showed a clear seasonality with a winter maximum and a summer minimum in both towns. The mutagenic potency of air correlated well with the air BaP and total PAH content in Ajka, but not in Papa. The amounts of extractable organic material and mutagenic potency as calculated for unit quantity of airborne particulate matter was higher in the Papa samples. Similar differences between the two towns were observed in the case of fallen dust, too. On the basis of examination of emitted dust, it can be stated, that in the mutagenicity of urban air, the aluminum plant emission plays a considerably higher role than the power plant emission, which is the main component of air dust pollution in Ajka.

Air Pollutants↗
PubMed1987

Different electrophoretypes of human rotaviruses in Hungary.

Polyacrylamide gel electrophoresis of rotaviral double-stranded ribonucleic acid (RNA) extracted directly from faecal specimens collected in three different parts of Hungary was applied to characterize and distinguish 21 randomly selected viral isolates. This technique made it possible to define 7 different electrophoretypes. Of the isolates 11 exhibited an identical "long" electrophoretic migration pattern. "Short" RNA pattern was found in two cases, and one atypical rotavirus was also revealed. This is the first description of rotavirus RNA electrophoretypes in Hungary.

Child, Preschool↗
PubMed1987

Mutagenicity studies on terbacil, an uracil-analogue pesticide.

The possible mutagenic effect of the uracil analogue pesticide Terbacil was examined on Drosophila melanogaster and in mammalian cell culture. Terbacil did not prove to be mutagenic in the sex-linked recessive lethal mutation test, and it did not cause an increase in sister chromatid exchange in CHO cells. It cannot be considered a chromosome-mutagenic compound. Considering the procaryote studies known from the literature, it is assumed that Terbacil does not represent a genotoxic hazard for the persons involved either in its production or its use.

Animals↗
PubMed1986

Virulence factors of Escherichia coli. III. Correlation with Escherichia coli pathogenicity of haemolysin production, haemagglutinating capacity, antigens K1, K5, and colicinogenicity.

A total of 1156 Escherichia coli strains including 489 faecal, 384 urinary, 283 other extraintestinal isolates was compared for haemolysin production (Hly), mannose resistant haemagglutinating activity (MRA), presence of antigens K1 and K5 and colicinogenicity (Col). K1 capsule which was demonstrated only in a few serogroups (O1, O2, O7, O18) occurred more frequently among extraintestinal (32.1%) than among faecal (4.3%) or urinary (7.3%) isolates. In the incidence of antigen K5 there was no difference between faecal and urinary (3.3%; 3.1%) or between urinary and other extraintestinal (5.3%) isolates belonging mainly to serogroups O2, O6, O18 and O75. Col+ isolates occurred frequently in all samples (23.5% of faecal, 31.7% of urinary and 43.4% of other extraintestinal strains), they being significantly more frequent in serogroups O1, O2, O7, O18 than in others. A close association existed between K1+ and Col+ properties, mainly (24.4%) among strains isolated from extraintestinal sources other than urine. The frequent coexistence of K1+ and Col+ in serogroups O1, O2, O7, O18 offers a further explanation for the extraintestinal pathogenicity of these serogroups. Neither Hly+ and K1+, nor Hly+ and Col+ were associated. MRA+ and K1+ correlated mainly in serogroups O1, O2 but never occurred simultaneously in serogroup O18. Connection between MRA+ and Hly+ was not associated with other virulence factors (K1, Col). The results showing a close connection among certain serogroups (O1, O2, O4, O6, O7, O18) and certain markers of pathogenicity (MRA, Hly, K1, Col) support the concept that E. coli strains have a clonal connection.

Antigens, Bacterial↗
PubMed1984

Virulence factors of Escherichia coli. I. Mannose resistant haemagglutinating capacity is associated with serogroup but not with site of infection.

Different serogroups of Escherichia coli strains originating from faeces (patients with enteritis 244, healthy individuals 225), urine (pyelonephritis 111, cystitis 130, asymptomatic bacteriuria 59) and other extraintestinal sources (blood 30, cerebrospinal fluid 15, wound 13, autopsy material 9, umbilicus 8, vagina 20, throat 13 and nose swabs 5) were examined for mannose resistant haemagglutination of human A erythrocytes (MRHA hum.). The most frequent serogroups were O1, O2, O4, O6, O7, O18 and O75 uniformly among faecal (26.5%), urinary (34%) and other extraintestinal (57.9%) strains. Haemagglutinating activity was significantly more frequent in these serogroups than in others (p less than 0.001). There was no significant association between MRHA positivity and origin of the serogroups. It has been concluded that MRHA hum. property of an E. coli strain depends on its serogroup rather than its origin. As these serogroups are the most frequent in the intestinal flora, it is assumed that their physiological function is to colonize the bowel and their pathological significance is to provide a source for extraintestinal infection.

ABO Blood-Group System↗
PubMed1982

Inhibition by an antioxidant of the carcinogenic effect of nitrosomorpholine formed in vivo.

In earlier studies, it was found that the new antioxidant, MTDQ-DA ((6,6-methylene-bis)2,2-dimethyl-4-methane-sulphonic acid sodium salt-1,2-dihydroquinoline) (Patent No. 3172) prevented in vivo nitrosation and the acute hepatotoxicity of morpholine plus sodium nitrite in rats. The aim of the present experiments was to examine whether MTDQ-DA could decrease the frequency of tumours induced by simultaneous administration of morpholine and nitrite in the lung adenoma assay. Forty CFLP mice were used in each group and they were killed 8 months after treatment. In group A, treated with 1000 mg/kg morpholine i.g., and in Group B (untreated control) a similar frequency of lung adenomas could be observed (0.13 adenomas/mouse). After the combined i.g. treatment in Group C (MOR, 1000 mg/kg + nitrite, 125 mg/kg), the incidence of lung adenomas was found to be 16.58/mouse. A single dose of MTDQ-DA (500 mg/kg) added to the group C treatment reduced the lung adenoma frequency to 2.47/mouse (Group D). As MTDQ-DA shows neither toxic nor mutagenic effects and inhibits the in vivo nitrosation in both hepatotoxicity and lung adenoma assays, it may be considered to be a potential food additive.

Animals↗
PubMed1980

Endogenous formation of N-nitrosomorpholine from a new fungicide.

Trimorphamide [N-(1-formamido-2,2,2-trichloroethyl)morpholine], with an acute LD50 of 990 mg/kg, is a systemic fungicide under development. To examine its possible nitrosation in vivo, CFY rats were treated i.g. with 326 mg/kg trimorphamide, suspended in 40 mg/kg sodium nitrite. after 1 hour, another 40 mg/kg sodium nitrite was given i.g. and the animals were killed an hour later. The nitroso derivative was extracted by dichloromethane from the gastric content, after steam distillation. The samples were analysed by gas chromatography (GC) and mass spectrometry (MS). N-Nitrosomorpholine (NMOR) was identified by comparison with authentic samples, both by GC and MS, using retention times and electron impact mass spectra. Quantification was carried out by GC-MS-specific ion monitoring, using the m/e 116 peak. The yield of NMOR ws found to be 0.65%. By the use of the Ames test, NMOR formed in vivo proved to be strongly mutagenic for the strains TA 1535 and TA 100, both in presence and absence of S-9 fraction. Tumour-bearing animals, treated by sodium nitrite plus trimorphamide, are being analysed.

Animals↗
PubMed1975

Induction of malignant lymphomas in swiss mice by n-nitroso compounds formed in vivo.

Malignant lymphomas were induced in Swiss mice treated intragastrically with methyl-2-benzimidazole carbamate (MBC, BCM, Carbendazim) and given sodium nitrite in their drinking water. The tumours appeared between 82 and 164 days after the beginning of treatment. In 30 mice given MBC and sodium nitrite, 10 lymphosarcomas were found. In the proliferating tumour cells, intracytoplasmic type-A virus particles were demonstrated by electron microscopy. No tumours were found in animals given MBC only. The findings demonstrate the possibility of in vivo formation of N-nitroso derivatives from MBC.

Animals↗
PubMedDate not supplied

[A full-term second abdominal pregnancy].

A case is described of abdominal pregnancy in a 27-year-old patient with a previous salpingectomy of the right tube performed because of tubal pregnancy. The patient was hospitalized in the 22nd to the 28th week of pregnancy on suspicion of imminent abortion. Clinical and ultrasound examinations indicated intrauterine pregnancy, by just before the expected date of delivery, clinical symptoms suggested abdominal pregnancy. The oxytocin test was negative. Following laparotomy a live normal girl was born, weighting 3200 g. The placenta was inserted at the front wall and the right horn of the uterus from the outer side. In the right horn there was an opening at the site of the oviduct excision. Because of profuse bleeding, the uterus was amputated supravaginally.

Adult↗