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M D APPLEMAN

Publications and source records attributed to M D APPLEMAN.

At least 19 recordsLinked to original sources
PubMed1964

IMPROVED STAPHYLOCOCCUS MEDIUM NO. 110.

Most media have been unreliable when used to determine the number of coagulase-positive staphylococci in meat pot pies in the presence of overwhelming numbers of Bacillus cells. Various metabolic inhibitors were tested to determine whether they would suppress the growth of Bacillus cells without appreciably affecting the staphylococcal growth. The use of Staphylococcus Medium No. 110 modified by the addition of 0.75 mM sodium azide appears to be practical for the isolation of small numbers of coagulase-positive staphylococci from frozen meat pot pies.

Bacillus↗
PubMed1963

Toxic effect of staphylococcal lysins for goldfish.

Goldfish died within 24 hr after intraperitoneal injections of 0.2 ml of Seitz filtrates of hemolytic Staphylococcus aureus cultures grown on Dolman and Wilson medium under increased CO(2) pressure for 72 to 96 hr. Two lethal toxins differing in heat sensitivity, antigenicity, and degree of toxicity were demonstrated. Studies of the relationship between the lethal factors and the hemolysins in the filtrates suggested that alpha- and beta-lysin were responsible for the lethal effects. Filtrates of nonhemolytic staphylococcal cultures were innocuous. Goldfish were suitable animals for detecting toxicity in staphylococcal culture filtrates and for quantitative studies of the toxins. The results were highly reproducible.

Animals↗
PubMed1963

DEGRADATION OF ERGOTHIONEINE BY CELL-FREE EXTRACTS OF ALCALIGENES FAECALIS. II. PRODUCTION OF GLUTAMIC ACID.

Booth, James S. (University of Southern California, Los Angeles) and Milo D. Appleman. Degradation of ergothioneine by cell-free extracts of Alcaligenes faecalis. II. Production of glutamic acid. J. Bacteriol. 85:654-657. 1963.-On the basis of oxidation and paper chromatographic procedures, glutamic acid was identified as the end product of ergothioneine degradation by cell-free extracts of Alcaligenes faecalis. Hydrogen sulfide and ammonia yields were determined. Several differences between the metabolism of whole cells and cell-free extracts were noted. Cleavage of the imidazole ring by cell-free extracts appeared to be hydrolytic rather than oxidative.

Alcaligenes↗
PubMed1962

Complete reduction of tellurite to pure tellurium metal by microorganisms.

Tucker, Fayne L. (University of Southern California, Los Angeles), John F. Walper, Milo Don Appleman, and Jerry Donohue. Complete reduction of tellurite to pure tellurium metal by microorganisms. J. Bacteriol. 83:1313-1314. 1962-The black precipitate produced in the presence of potassium tellurite by growing cells of Streptococcus faecalis N83 and Corynebacterium diphtheriae was shown, by X-ray diffraction analysis, to consist of metallic tellurium. The metal was not complexed, to any significant degree, with any organic material.

California↗