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Biomedical subjects

M D Barros

Publications and source records attributed to M D Barros.

11 recordsLinked to original sources
PubMed2001

Pulmonary function response to EDTA, an additive in nebulized bronchodilators.

BACKGROUND: Some nebulized bronchodilator solutions contain additives, such as EDTA, benzalkonium chloride (BAC), or both. OBJECTIVE: Although BAC-induced bronchoconstriction has been well documented in patients with asthma, there is no information on the effects of EDTA on FEV(1) when inhaled in the amounts that would be administered during emergency department treatment of asthma. METHODS: Eighteen subjects with stable asthma and airway responsiveness to methacholine were randomly assigned to inhale up to four 600-microg nebulized doses of EDTA, BAC (positive control), and normal saline (placebo) in a double-blind crossover manner on separate days. FEV(1) was measured 15 minutes after each dose. Treatments were repeated every 20 minutes until FEV(1) decreased by 20% or greater or a maximum of 4 doses were administered. RESULTS: Mean +/- SD maximum percent decrease in FEV(1) was 1.8% +/- 5.8% after EDTA, 16.6% +/- 13.9% after BAC, and 3.6% +/- 8.2% after placebo (P <.001); there was no significant difference between EDTA and placebo. CONCLUSION: The amount of EDTA contained in maximum recommended doses of nebulized bronchodilators does not induce bronchospasm. In contrast, BAC induces clinically important bronchospasm, which could decrease the efficacy of a bronchodilator during an emergency.

Adolescent↗
PubMed2001

[Child and adolescent mortality due to external causes: trends from 1979 to 1995].

OBJECTIVE: To analyze the magnitude and trends of mortality due to external causes in specific age groups, from 0 to 19 years old in a population living in the city of Recife, Brazil, from 1979 to 1995. METHODS: Data from the Mortality Data System of the Ministry of Health and Health Department of Pernambuco State was used. The studied population, aged 0 to 19 years old, represented 41.8% of total city population in 1991. A time series exploratory ecological model was created to analyze trends in mortality coefficients due to external causes. Using simple linear regression these coefficients were assessed in specific age groups categorized by gender. RESULTS AND CONCLUSIONS: In the time series, mortality coefficients due to external causes showed an increasing trend, in particular due to homicides among adolescents, where the coefficient increased on average 3.05 per year, yielding a relative increase of 601, 3% over the study period. In 1995, more than 90% of these homicides were perpetrated with fire weapons. These data reinforce the seriousness of this problem and the need to deal with it, taking into account the difficulties in determining the causes of violence.

Accidents, Traffic↗
PubMed2001

Serodiagnosis of chronic Chagas infection by using EIE-Recombinant-Chagas-Biomanguinhos kit.

A kit based on an enzyme immunoassay, EIE-Recombinant-Chagas-Biomanguinhos, developed by the Oswaldo Cruz Foundation, was evaluated for the serodiagnosis of chronic Chagas disease. Evaluation was performed with 368 serum samples collected from individuals living in an endemic area for Chagas disease: 131 patients in the chronic phase with confirmed clinical, epidemiological, and serological diagnosis (indirect immunofluorescence, indirect hemagglutination or enzyme-linked immunosorbent assay) and 237 nonchagasic seronegative individuals were considered negative control. The EIE-Recombinant-Chagas-Biomanguinhos kit showed high sensitivity, 100% (CI 95%: 96.4-100%) and high specificity, 100% (CI 95%: 98-100%). The data obtained were in full agreement with clinical and conventional serology data. In addition, no cross-reaction was observed with sera from patients with cutaneous (n=14) and visceral (n=3) leishmaniasis. However, when these sera were tested by conventional serological assays for Chagas disease, cross-reactions were detected in 14.3% and 33.3% of the patients with cutaneous and visceral leishmaniasis, respectively. No cross-reactions were observed when sera from nonchagasic seronegative patients bearing other infectious disease (syphilis, n=8; HTLV, n=8; HCV, n=7 and HBV, n=12) were tested. In addition, sera of patients with inconclusive results for Chagas disease by conventional serology showed results in agreement with clinical evaluation, when tested by the kit. These results are relevant and indicate that the referred kit provides a safe immunodiagnosis of Chagas disease and could be used in blood bank screening.

Adolescent↗
PubMed2001

[Underlying cause of death from external causes: validation of official data in Recife, Pernambuco, Brazil].

OBJECTIVE: To validate the underlying cause of death recorded on the death certificates for individuals under 20 years of age who died from external causes in 1995 in Recife, Pernambuco, Brazil. METHODS: We divided the study into two stages, coding and validation. In both stages we compared the official data concerning causes of death to the data we obtained during our study. We grouped the death certificates into 5 broad categories according to the cause of death; we later subdivided them into 14 categories. We also individually compared the death certificates applying the four-digit system of the International Classification of Diseases, Ninth Revision (ICD-9). We assessed the agreement between the official data and our data in terms of sensitivity and the kappa coefficient. We took as the standard the categorization of the cause of death that we had made during our investigation. RESULTS: In the coding stage, considering all the external causes of death, the overall agreement between the official data and our study data was 94% for the 5 categories, 92% for the 14 categories, and 81% for the four-digit ICD-9 system. In the validation stage the overall agreement was 94% for the 5 categories, 91% for the 14 categories, and 73% for the four-digit ICD-9 system. CONCLUSIONS: Our results suggest that for the death certificates to be reliable, the Institute of Legal Medicine must fill them out following recommended standards. In addition, hospitals and police departments must use greater care in completing the transfer slips that accompany the bodies that are sent to the Institute. More accurate data need to be generated and disseminated for a society to better understand its patterns of violence.

Adolescent↗
PubMed1997

Ocular-specific delivery of timolol by sequential bioactivation of its oxime and methoxime analogs.

S-(-)- Timolol maleate was oxidized, using the modified Pfitzner-Mofatt method, to the corresponding keto analog, which was then coupled with either hydroxylamine or methoxyamine in the same reaction medium. The products separated, timolone oxime (TO) or timolone methoxime (TMO), were found to be a mixture of both E and Z isomers with the Z isomer in higher concentration. Both isomers could be separated on silica column. No isomerization of any of the isomers could be detected whether in buffers or biological fluids. TMO salts were found to be stable in slightly acidic buffer. The Z isomer of TMO is more stable than the E isomer. Both TO and TMO showed pronounced reduction of the intraocular pressure (IOP) in normotensive rabbits, when instilled into the conjunctival sac. Reduction of IOP caused by either TO or TMO was higher than the reduction produced with the same dose of timolol maleate. Equal doses of any of the TMO isomers or the mixture of isomers gave almost the same percent reduction of IOP. TMO and TO did not show cardiovascular effects when administered intravenously to rabbits or rats. Both are good candidates to be used for topical management of glaucoma without producing systemic side effects.

Administration, Topical↗
PubMed1997

Ocular-specific chemical delivery systems of betaxolol for safe local treatment of glaucoma.

Novel ketomethoxime (BMO) and oxime (BO) analogs of betaxolol (B) were prepared through the oxidation of betaxolol, followed by quenching of the ketone with the appropriate oxyamine. The Z isomers were kinetically favored and thermodynamically more stable. Isomerization to reach an equilibrium mixture of Z/E was observed for all pure isomers in buffers. Equilibration is much faster, however in biological fluids. Ocular administration of any of the oxime derivatives, delivers betaxolol specifically to the eye tissues, with the highest concentration in the iris ciliary body. Both BMO and BO, when applied topically, showed marked reduction of intraocular pressure (IOP) in normotensive rabbits. No effect on isoproterenol-induced tachycardia in rabbits and rats were observed, even after iv. administration. Very mild eye irritation, which was less than that of betaxolol hydrochloride, was observed particularly with BMO maleate, which is an excellent candidate for safe treatment of glaucoma.

Administration, Topical↗
PubMed1992

Mutational analysis of a plant heat shock element.

A total of 32 mutations were generated within the TATA-proximal site 1 (-72 to -47) of soybean heat shock gene Gmhsp17.5E in order to functionally define the optimal configuration of sequences within the heat shock element (HSE). Mutants were tested in vivo utilizing sunflower tumors transformed by a T-DNA based vector. Promoter activity was determined by S1 nuclease hybrid protection analysis of tumor transcripts. A total of five repeats (5'-nGAAn-3' or 5'-nTTCn-3') which comprise the HSE at site 1 were required for full transcription induction by heat stress. Analysis of non-conserved bases flanking the central trinucleotide block indicated that 5'-aGAAg'-3' is the optimum sequence for the 5 bp repeat.

Base Sequence↗
PubMedDate not supplied

[Fulfilling of variables in the declarations of external cause of death of children and adolescents in Recife from 1979 to 1995].

This article analyzes the completion of death certificates related to external causes in children and adolescents residing in Recife, Pernambuco State, Brazil, from 1979 to 1995. The analysis focused on the extent to which the following variables were filled in: personal data (occupation, level of schooling, medical care, confirmation of diagnosis by post-mortem, and type of violence) and place (location and municipality of both occurrence and death). Using the chi-squared method, quantitative and/or qualitative flaws were found in most of the variables analyzed. In 1995, the "schooling" and "medical care" variables were recorded in only 5.7% and 17.9% of cases, respectively. Mismatches were observed between data on place of death and medical care, as well as between place of accident and number of accidents. The results suggest a dissociation between the objective of including the variable in the death certificate and its social function. The study provides the public sector with support for improved collecting and critical analysis of data in the mortality information system.

Adolescent↗
PubMedDate not supplied

[Risk factors, clinical and laboratory aspects of asthma in children]

OBJECTIVE: To evaluate the clinical and laboratory aspects and the risk factors associated with asthma in children treated at the Pediatric Outpatient Clinic of Hospital Universitário Júlio Müller. METHODS: A case-control study including 59 asthmatic children (cases) and 104 nonasthmatic children (controls). The following factors were considered for risk analysis: parentś level of education, domestic exposure to allergens, passive smoking, breast feeding, low income, and family history of allergy. Samples of blood were collected for hemogram and to determine the total serum IgE as well as the one specific to allergens. Immediate hypersensitivity skin tests were performed with puncture for the detection of the following allergens: house dust mite, animals, molds, and cockroaches with positive (histamine) and negative controls (physiologic solution). A logistic regression model was used to calculate the odds ratio (OR) and 95% confidence intervals (95%CI) adjusted for risk factors and for confounding factors. RESULTS: Among the risk factors studied, sex, parentś low level of education, low income, length of the breast feeding period, and passive smoking were not associated with the presence of asthma. The domestic exposure to allergens was similar in both groups except for the higher frequency of pets at the homes of control patients (chi-square=16.9; P<0.05). Paternal history of rhinitis was the only association with asthma (OR=3.33; 95%CI: 1.03-11.17; P<0.05). The asthmatic children presented higher frequency of positive reactions to skin tests than the controls, mainly to house dust mites: Dermatophagoides pteronyssinus (69.5%), Dermatophagoides farinae (59.3%) and Blomia tropicalis (59.3%); cockroaches: Periplaneta americana (59.3%), and cat: Felis domesticus (37.3%), with OR between 11.2-21.0; P<0.05. Eosinophilia and serum levels of total IgE were more elevated in the group of asthmatic children (P<0.05). The positivity of the specific IgE test for Dermatophagoides pteronyssinus and Blomia tropicalis was higher in the cases than in the controls (P<0.05). The multivariate analysis showed that sensitization to the allergens produced by cockroaches (OR=9.26; 95%CI: 2.59-33.4), animals (OR=3.93; 95%CI: 1.05-14.67) and house dust mites (OR=3.74; 95%CI: 1.18-11.8) were the most important risk factors for asthma. CONCLUSIONS: The sensitization to indoor allergens, mainly to house dust mites, cockroaches, and cats showed a strong association with asthma in this study.

Journal Article↗
PubMedDate not supplied

Study of colostrum of a patient with selective IgA deficiency.

In a study of immunological factors in colostrum, we detected an 18-year old primipara with selective IgA deficiency and with a history of 3 previous hospitalizations for bronchopneumonia. Peripheral blood studies showed: IgG = 1240 mg%; IgM = 160 mg, and undetectable IgA on two different occasions. B lymphocyte measurement by immunofluorescence for membrane immunoglobulins, T lymphocyte determination by rosette formation with sheep red cells, and T lymphocyte determination with monoclonal antibodies of the Ortho series showed normal results. Salivary IgA was undetectable. The colostrum results were: undetectable IgA (mean, 19.2 g/l); IgM, 22,2 g/l (mean, 2.3 g/l); IgG, 1.3 g/l (mean, 0.7 g/l), and lysozyme, 214.2 mu/ml (mean, 157.1 mu/ml). The very high IgM levels detected in the colostrum of these patients are interpreted to be a compensatory factor for the lack of IgA.

Adolescent↗