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Biomedical subjects

M D Darrow

Publications and source records attributed to M D Darrow.

2 recordsLinked to original sources

Ordering and understanding the exercise stress test.

The exercise stress test is a useful screening tool for the detection of significant coronary artery disease. Documentation of the patient's symptoms, medications, past and current significant illnesses, and usual level of physical activity helps the physician determine if an exercise stress test is appropriate. The physical examination must include consideration of the patient's ability to walk and exercise, along with any signs of acute or serious disease that may affect the test results or the patient's ability to perform the test. The test report contains comments about the maximal heart rate and level of exercise achieved, and symptoms, arrhythmias, electrocardiographic changes and vital signs during exercise. This report allows the clinician to determine if the test was "maximal" or "submaximal." The quality of the test and its performance add to the validity of the results. The conclusion section of the test report indicates whether the test results were "positive," "negative," "equivocal" or "uninterpretable." Further testing may be indicated to obtain optional information about coronary artery disease and ischemic risk if the test results were equivocal or uninterpretable.

Contraindications↗

Characterization of yeast iso-1-cytochrome c mRNA.

The iso-1-cytochrome c mRNA has been identified by hybridization of a 32P probe prepared from a plasmid containing the iso-1-cytochrome c gene to RNA size-fractionated on agarose gels and transferred to paper. A hybridization band was visible with RNA prepared from wild type cells, but not with RNA prepared from an iso-1-cytochrome c deletion mutant. RNA prepared from cells containing a nonsense mutation in the iso-1-cytochrome c gene showed reduced levels of hybridization. The RNA that hybridized to the probe was 700 +/- 50 nucleotides in length and was polyadenylated. The cellular levels of this RNA were repressed by glucose, and this repression was achieved within 5 min after glucose addition to a derepressed culture. No precursors of this RNA were detected in wild type cells or in an RNA1 mutant, temperature-sensitive for RNA metabolism. The length of the 3' noncoding region of this RNA was determined to be 200 +/- 25 nucleotides (excluding the poly(A) tail) and the 5' noncoding region was estimated to be about 120 nucleotides in length.

Base Sequence↗