PubMed Health⌕ Search

Biomedical subjects

M D McNeely

Publications and source records attributed to M D McNeely.

14 recordsLinked to original sources
PubMed1990

The optimum urine collections for the detection and monitoring of Bence Jones proteinuria.

Twenty patients with malignant disease and Bence Jones (BJ) proteins were studied to determine the optimum urine collections for the detection and monitoring of light chain proteinuria. A 24-hour urine protein collection was followed by individual collections of each sequentially voided specimen over the same time interval. Samples were analyzed quantitatively for protein, and protein electrophoresis was performed on each specimen. Only one patient had BJ protein nondetectable by protein electrophoresis in the early morning specimen. Six patients had one or more random specimens (excluding the early morning specimens) absent for BJ protein on protein electrophoresis. Three patients had nondetectable protein on electrophoresis of the 24-hour specimen despite having some random specimens positive. All random specimens with protein values exceeding 0.20 g/L had BJ protein visibly detectable on electrophoresis. Thirteen specimens with protein less than 0.05 g/L still had BJ protein detected by electrophoresis. There was a linear relationship between the early morning protein concentration and the total 24-hour urinary protein production. The authors conclude that early morning specimens or 24-hour urine collections are preferable for the detection and monitoring of light chain proteinuria. These collection methods are not mutually exclusive because there are individual patients who will be negative in one collection but positive in the other.

Aged↗
PubMed1983

Computerized interpretation of laboratory tests: an overview of systems, basic principles and logic techniques.

The ever-increasing volume and complexity of laboratory testing demands that interpretive reporting schemes be initiated. However, because of the massive volume of laboratory data, computers must be employed in this task. To design such programs, a clear understanding of the diagnostic process is required. There are numerous factors which must be respected for reports to be designed in an optimal fashion. Reports can vary in complexity from simple normal range listings to elaborate mathematical modelling of physiologic processes. A variety of well-studied logic techniques can be employed. These features have been studied during the development of the LABDOC computerized reporting system.

Chemistry, Clinical↗
PubMed1977

Creatine kinase and its isoenzymes in the serum of women during pregnancy and the peripartum period.

In serum obtained from 28 women before, during, and after normal labor and delivery, creatine kinase activity was seen to be distinctly elevated immediately after labor and 24 h later, but had returned to normal six weeks later. In most cases the increase was due to the MM isoenzyme and was attributed to skeletal-muscle damage associated with labor. In 15 cases, the BB isoenzyme was observed, and in three patients the MB isoenzyme. Cord blood, which contains all three isoenzymes, may be the source of the MB and BB isoenzymes. Uterine muscle contains exclusively BB isoenzyme, and therefore uterine muscle damage is a likely source of the serum BB isoenzyme. The relationship among these isoenzymes in serum after delivery must be recognized, to avoid misdiagnosing myocardial infarction at this critical time.

Creatine Kinase↗
PubMedDate not supplied

Automated bichromatic analysis of serum ceruloplasmin.

An automated micromethod is described for measurement of serum ceruloplasmin by assay of its p-phenylenediamine oxidase activity using the Abbott bichoromatic analyzer. Ten mul of serum are mixed with 250 mul of p-phenylenediamine (PPD) substrate (9.2 mmole per liter) in acetate buffer (0.1 mole per liter, pH 5.45). Spectrophotometric measurements of the rate of formation of the purple oxidation porduct of PPD are performed after a 10 min delay for thermal equilibration at 37 degrees and for avoidance of the lag-phase of the enzymatic reaction. The coefficients of variation of replicate analyses of normal serum by this technique are 1.1 percent (within-the-run) and 3.3 percent (day-to-day). Measurements of ceruloplasmin concentrations in serums from 75 patients by this automated method provided close correlation with measurements by a manual reference procedure (correlation coefficient=0.973). The mean concentration of ceruloplasmin in serums from 64 healthy men was 29 mg per dl (central 95th percentile limits=22 to 40 mg per dl).

Autoanalysis↗