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M De Waele

Publications and source records attributed to M De Waele.

At least 73 records · Page 4Linked to original sources
PubMed1985

Unusual nonimmunoglobulin-containing inclusions in a case of follicular large cell lymphoma.

Intracytoplasmic inclusion bodies were found in a case of follicular large cell lymphoma. They did not react with anti-immunoglobulin antisera and showed no enzyme reactivity. On electron microscopy the inclusions consisted of loosely packed fibrillar material not surrounded by a membrane or by rough endoplasmic reticulum. They were found only in the large lymphomatous cells. Immunocytochemistry showed a reactivity of these cells with anti-HLA-Dr and the OKT10 monoclonal antibodies. The nature of the inclusions remains unknown. They differ significantly from those described in the literature in cases of B-cell lymphoproliferative disorders.

Antibodies, Monoclonal↗
PubMed1985

Activated T-cells with suppressor/cytotoxic phenotype in acute Toxoplasma gondii infection.

The mononuclear cells in the peripheral blood of 17 adults with acute phase toxoplasmosis were characterized with monoclonal antibodies. The absolute number of lymphocytes was increased by the presence of high numbers of T-cells with a surface phenotype of T-cytotoxic/suppressor cells (OKT3+, OKT8+). Shortly after the onset of the infection these cells expressed activation antigens (OKT10+, OKIa+). These changes were more pronounced in patients with lymphadenopathy, but were also present in those without symptoms of the disease. The activation antigens disappeared in the first weeks after onset of the disease but the reversed OKT4/OKT8 ratio persisted for 2 to 4 months. It generally normalized in parallel with clinical recovery of the patients. In one patient, however, a reversed OKT4/OKT8 ratio was present for more than 1 year after the disappearance of the symptoms.

Adult↗
PubMed1984

Intracellular inclusion bodies in 14 patients with B cell lymphoproliferative disorders.

Two types of intracytoplasmic inclusion were detected by immunofluorescence microscopy in 12 patients with chronic lymphocytic leukaemia and two patients with a leukaemic phase of well differentiated lymphocytic lymphoma. Further analysis with light- and electron microscopy, showed that most inclusion bodies were rod-like crystalline structures. However, in three patients they consisted of amorphous vesicular precipitates. Immunological studies revealed the presence of immunoglobulins of the same class and type at the cell surface as well as in the inclusion bodies. The monoclonal immunoglobulins were all of lambda type except in two cases. The origin of immunoglobulin inclusion bodies in B cell malignancies is discussed in relation to published data and our own observation in one patient followed during treatment.

Aged↗
PubMed1983

Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.

Colloidal gold was used as a marker for the light microscopic detection of lymphocyte cell surface antigens with monoclonal antibodies. Suspensions of peripheral blood leukocytes were first incubated with monoclonal mouse antibodies and then with colloidal gold-labeled goat anti-mouse antibodies. The cells were fixed and cytocentrifuge preparations or smears were made. Granulocytes and monocytes were then labeled by the cytochemical staining of their endogenous peroxidase activity. Lymphocytes reacting with the monoclonal antibody had numerous dark granules around the surface membrane. With electron microscopy, these granules appeared as patches of gold particles. This immunogold staining method proved to be a reliable tool for the enumeration of T-lymphocyte subpopulations in peripheral blood. The results were almost identical to those obtained with immunofluorescence microscopy. The procedure can also be applied on small volumes of capillary blood. This constitutes a good microtechnique for the determination of lymphocyte subsets in children.

Adult↗
PubMed1983

Cytochemical profile of immunoregulatory T-lymphocyte subsets defined by monoclonal antibodies.

Immunogold staining in combination with enzyme cytochemistry was used to determine the cytochemical profile of the immunoregulatory T-lymphocyte subpopulations defined by the monoclonal antibodies OKT3, OKT4, OKT8, and OKM1 in normal peripheral blood. Leukocyte suspensions were first incubated with the monoclonal mouse antibodies and then with colloidal gold-labeled goat antimouse antibodies. The cells were fixed and cytocentrifuge preparations were made. Cytochemical reactions for the detection of peroxidase, acid alpha-naphthyl acetate esterase, acid phosphatase, and beta-glucuronidase were performed on these preparations. Under light microscopy, lymphocytes reacting with the monoclonal antibodies had numerous dark granules around their surface membrane. In the cytoplasm the intracellular enzymatic activities were stained. The T-lymphocytes were characterized by a dot-like activity for the three enzymes. No significant difference could be found between the cytochemical profile of the T-helper (OKT4 positive) and T-cytotoxic suppressor cell populations (OKT8 positive). A few cells with lymphocyte morphology reacted with the OKM1 monoclonal antibody. Their cytochemical characteristics were different from those of mature T-cells (OKT3 population) or mature monocytes. From the comparison of their cytochemical characteristics, we can conclude that there is little correlation between the immunoregulatory T-lymphocyte subsets defined by these monoclonal antibodies and those defined by Fc receptors.

Acid Phosphatase↗
PubMed1982

Intracellular immunoglobulin distribution of bone marrow plasma cells as a diagnostic aid for primary amyloidosis.

In order to classify the underlying disorder in four patients with biopsy-proven amyloidosis without overt monoclonal gammopathy, the cytoplasmic immunoglobulin (Ig) distribution of bone marrow plasma cells was evaluated using direct immunofluorescence microscopy. This procedure revealed the presence of a monoclonal proliferation of light chain containing plasma cells, and thus led to the diagnosis of immunoglobulin-related amyloidosis.

Aged↗
PubMed1982

Colloidal gold as a marker for the light microscope detection of leukocyte cell surface antigens with monoclonal antibodies.

Colloidal gold was used as a marker for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies. Leukocytes were first incubated with monoclonal mouse antibodies, and then with colloidal gold-labeled goat-anti-mouse antibodies. The cells were fixed and stable preparations were made. Positive cells displayed numerous dark granules all over their surface membrane. In electron microscopy, these granules appeared as patches of gold particles. This immunogold staining method proved to be a reliable tool for the enumeration of T-lymphocytes and their subclasses. Accurate cell recognition, based on morphology and cytochemistry, permitted rapid cell counting. The procedure was also applied on small volumes of whole blood. This approach was used as a microtechnique for the enumeration of lymphocyte subpopulations in pediatric patients. In normal peripheral blood, the intracellular enzymatic activities of the immunogold-labeled lymphocytes were detected by the classical cytochemical reactions. The staining patterns for acid alpha-naphthyl acetate esterase, acid phosphatase and beta-glucuronidase were identical to those found in smears of unlabeled cells. The cytochemical profile of the OKT-defined normal lymphocyte subpopulations was established.

Animals↗
PubMed1982

Two monoclonal antibodies (OKIa1 and OKT10) for the study of the final B cell maturation.

OKT10 is a monoclonal antibody which recognizes an antigenic structure of 45,000 daltons which is present on precursor T and B cells, activated T cells and some monocytes. In this study, this antibody was shown to react strongly with the surface membrane of plasma cells. In addition, OKT10 can be used to study final B cell maturation since the OKT10 antigen is expressed on mature lymphocytes and lymphoplasmacytic cells. Acquisition of OKT10 reactivity occurs in an inverse or opposite relationship to the presence of Ia-like antigenic expression. Therefore, these two antigens can be employed as complementary opposite reactants for the study of mature B cell malignancies, such as CLL, multiple myeloma, and Waldenström malignancy.

Animals↗
PubMed1981

Waldenström-like lymphoma with monoclonal IgG1-kappa: evidence of common clonal origin.

A patient with a pleomorphic lymphoplasmacytic bone marrow infiltration, a peripheral lymphocytosis and a monoclonal IgG1-kappa gammopathy is described. An antiidiotype serum, raised against the monoclonal serum protein showed a common clonal origin of the pleomorphic cell population. In the light of recent report on B-cell neoplasms associated with M-components, a hypothetical scheme of the cellular clonal event is proposed.

Aged↗
PubMedDate not supplied

Paravertebral schwannoma with high uptake of fluorodeoxyglucose on positron emission tomography.

A paravertebral mass was discovered in a 27-year-old woman, while investigating a painful shoulder and arm. CT, MRI and fine needle aspiration cytology (FNAC) pointed in the direction of a benign mass, but positron emission tomography (PET) showed a high uptake of [(18)F]fluorodeoxyglucose (FDG), which was indicative of a malignant lesion. Pathological analysis of the thoracoscopically resected tumour gave us the final diagnosis of a benign schwannoma. This report demonstrates that a high uptake of FDG in a non-malignant mediastinal tumour is possible.

Adult↗
PubMedDate not supplied

Gas chromatographic determination of drug levels for the evaluation of a combined hemoperfusion-hemodialysis treatment.

Recently, the use of a prolonged and combined procedure of hemoperfusion (HP) and hemodialysis (HD) was considered as a treatment for severely intoxicated patients caused by drug overdose. Working in cooperation with different hospitals, this laboratory has been involved with the toxicological analysis of biological samples taken during such a treatment. This paper describes the use of a combined HP/HD treatment, especially the evaluation of the system, by giving the analytical results obtained by gas chromatography. The methods utilized enable this application for routine toxicological analysis.

Adult↗
PubMedDate not supplied

Application of the combined use of fused silica capillary columns and NPD for the toxicological determination of codeine and ethylmorphine in a human overdose case.

The applicability of capillary gas chromatography to the toxicological analysis of extracts of human tissue samples was investigated for codeine and ethylmorphine in a case of drug overdose. After column extraction, the samples were injected onto a fused silica capillary column, using a direct injection technique. The nitrogen-phosphorus detector provided excellent stability and sensitivity without the need for extensive clean-up procedures.

Adult↗