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Biomedical subjects

M Deguchi

Publications and source records attributed to M Deguchi.

At least 55 records · Page 3Linked to original sources

Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.

We have recently isolated a rat homologue of the Caenorrhabditis elegans lin-10 product. Although rat lin-10 is expressed in the cytosol and membrane fractions of various tissues, it is distributed only in the membrane fraction in brain where it is enriched in the synaptic plasma membrane and postsynaptic density fractions. We have isolated here a rat lin-10-interacting protein from rat brain and identified it to be neurabin-II/spinophilin, which has recently been isolated as a protein interacting with protein phosphatase I and F-actin. Neurabin-II/spinophilin is ubiquitously expressed but enriched in brain, especially in the synaptic plasma membrane and postsynaptic density fractions. We discuss the physiological significance of the interaction of rat lin-10 with neurabin-II/spinophilin.

Actins↗

Molecular cloning and characterization of rat lin-10.

In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 encodes a small protein with one PDZ domain. The lin-10 product has no homology to known proteins. Here, we have cloned a rat homologue of the lin-10 product and characterized it. Rat lin-10 is ubiquitously expressed in various rat tissues and distributed in both the cytosol and membrane fractions. In brain, however, rat lin-10 is distributed only in the membrane fraction and enriched in the synaptic plasma membrane and postsynaptic density fractions. These results suggest that rat lin-10 is involved at least in synaptic functions in brain.

Animals↗

HLA-DRB1 alleles contribute to determining the prognosis of Japanese diabetes mellitus positive for antibodies to glutamate decarboxylase.

Diabetes mellitus positive for antibodies to glutamate decarboxylase is heterogeneous as far as the degree of impairment of endogenous insulin release, though antibodies to glutamate decarboxylase are the most useful marker for future insulin deficiency. To investigate what determines the prognosis of diabetes mellitus positive for antibodies to glutamate decarboxylase, we measured HLA-DRB1 alleles in three groups: 77 cases of insulin-dependent diabetes mellitus (IDDM), 44 of non-insulin-dependent diabetes mellitus (NIDDM) with secondary failure of oral hypoglycemic therapy, and 22 of NIDDM well controlled by diet and/or sulfonylurea agents. The proportion of susceptible and resistant alleles to IDDM determined the degree of insulin deficiency, and comparison of IDDM to NIDDM well controlled by diet and/or sulfonylurea agents revealed significant differences in DRB1*0405 (P < 0.05; RR = 2.82 and RR = 0.89, respectively) and DRB1*1502 (P < 0.001; RR = 0.02 and RR = 2.19, respectively). This study revealed that HLA-DRB1 alleles contribute to determining the prognosis of Japanese diabetes mellitus positive for antibodies to glutamate decarboxylase.

Adolescent↗

Increased proliferation of myoblasts after cyclic plasma perfusion of tumor-bearing rabbits.

Rabbits implanted with VX2 carcinoma and treated by cyclic plasma perfusion showed lower weight loss and longer survival than untreated rabbits. The urinary 3-methylhistidine/creatine ratio was less elevated in the treated rabbits than in the untreated rabbits. Myoblasts from untreated rabbits with VX2 carcinoma showed lower proliferation than those from normal rabbits, while those from rabbits treated by cyclic plasma perfusion showed intermediate proliferation. Furthermore, the DNA synthesis rate of myoblasts from treated rabbits was much better maintained than in the case of untreated rabbits.

Animals↗

Detection of human papillomavirus type 33 DNA in extragenital Bowen's disease with the polymerase chain reaction.

BACKGROUND: Data on the association of human papillomavirus (HPV) infection with extragenital Bowen's disease are very scarce. OBJECTIVE: To evaluate the prevalence of HPV infection in extragenital Bowen's disease showing histologically a number of koilocytes in the lesional epidermis, we studied formalin-fixed, paraffin-embedded tissues of 9 such cases. METHODS: HPV DNA was studied in such samples by the polymerase chain reaction (PCR) and in situ hybridization. RESULTS: Despite negative results with in situ hybridization, the PCR with HPV type 33 primer detected the presence of virus DNA in 2 out of 9 cases. CONCLUSION: As far as we know, this is the first report of the detection of HPV type 33 DNA in the lesional skin of extragenital Bowen's disease. The prevalence of HPV infection in extragenital Bowen's disease may be higher than expected, especially in cases histologically showing many koilocytes in the lesional epidermis.

Aged↗

Frequency of chromosome 7 gain in human breast cancer cells: correlation with the number of metastatic lymph nodes and prognosis.

Trisomy 7 has been reported in various malignant neoplasms, but there are no reports in breast cancer. In order to evaluate the contribution of chromosome 7 gain to breast cancer, we investigated the relationship of numerical abberation of chromosome 7 with clinicopathological variables and prognosis in seventy-nine breast cancer cases (invasive carcinomas) using the technique of fluorescence in situ hybridization (FISH) on paraffin-embedded sections. A significant correlation of the frequency of cells with extra copies of chromosome 7 (percent polysomy 7 cell score) was found with tumor size, regional lymph node status, tnm stage, histological extension, estrogen receptor (ER), and DNA ploidy. The number of metastatic lymph nodes was positively correlated with percent polysomy 7 cell score (correlation coefficient=0.623, p < 0.01). Furthermore, cases with a high percent polysomy 7 cell score had a shorter disease-free survival and overall survival times, especially in the lymph node-positive group. It was demonstrated that percent polysomy 7 cell value was closely associated with lymph node metastasis and prognosis and might be a useful prognostic predictor of breast cancer patients.

Adenocarcinoma↗

Strongyloidiasis associated with nephrotic syndrome.

We report a nephrotic syndrome patient with eosinophilia who developed ileus, epigastralgia and malabsorption due to strongyloidiasis which became symptomatic by steroid therapy. The patient was then treated with thiabendazole and recovered. A percutaneous renal biopsy revealed minimal change nephrotic syndrome. This renal injury may be brought on by severe infection of Strongyloides stercoralis. It is important to rule out strongyloidiasis prior to corticosteroid therapy to patients from eosinophilia endemic areas.

Abdominal Pain↗

Usefulness of metyrapone treatment to suppress cancer metastasis facilitated by surgical stress.

BACKGROUND: One causative factor of tumor metastasis enhanced by surgical stress is thought to be hypersecretion of endogenous glucocorticoids. This study evaluated the effectiveness of metyrapone treatment and adrenalectomy in preventing the harmful effects of glucocorticoids in the enhancement of tumor metastasis resulting from surgical stress. METHODS: The effect of dexamethasone on pulmonary metastasis of MRMT-1 cells and on the number of peripheral lymphocytes was evaluated in rats. To evaluate the suppressive effect of adrenalectomy and metyrapone treatment on operation-induced enhancement of metastasis, several parameters such as induction of pulmonary metastasis, serum corticosterone levels, and the number of blood lymphocytes and apoptotic thymocytes were determined. RESULTS: With dexamethasone treatment, the number of peripheral lymphocytes rapidly decreased; in contrast, pulmonary metastasis increased. The serum corticosterone level was doubled at 1 hour, apoptotic thymocyte numbers were increased about sevenfold at 3 hours and about fourfold at 6 hours, and blood lymphocyte numbers were decreased at 3 hours after laparotomy, which facilitated about a 10-fold increase in the pulmonary metastasis. These changes were almost completely suppressed by preoperative adrenalectomy and metyrapone treatment. CONCLUSIONS: Preoperative metyrapone treatment, which suppresses hypersecretion of endogenous glucocorticoids as a result of operation, modulates the enhancement of cancer metastases and may be an effective treatment.

Adrenalectomy↗

Effects of plasmapheresis in a rabbit model of cancer cachexia.

We developed a model of cancer cachexia by transplanting VX2 tumors into rabbits and investigated the effects of plasmapheresis in this model. AIS activity was identified in supernatants obtained from VX2 tumor by phenyl Sepharose chromatography. Fractions eluted with 0.5 M NaCl showed the ability to damage the red blood cell membrane and suppress immunity. Rabbits exhibited marked weight loss, anemia and immunodeficiency 40 days after transplantation of VX2 tumors. Plasmapheresis inhibited plasma-induced damage of the red blood cell membrane and suppression of immunity. Twice-weekly plasmapheresis resulted in gradual normalization of cellular immunity. Weight loss and survival were also improved. No side effects were observed.

Animals↗

Posterolateral fusion for isthmic spondylolisthesis in adults: analysis of fusion rate and clinical results.

This is a retrospective study of 83 consecutive adult patients with isthmic spondylolisthesis who underwent identical decompressive surgery combined with posterolateral spine fusion. We sought to determine factors that affect the fusion rate and clinical outcomes for adult patients with isthmic spondylolisthesis. The outcome of operative treatment for isthmic spondylolisthesis in adults has been poorly documented, as opposed to the treatment of children and adolescents. From 1989 to 1994, 83 consecutive adult patients (age 19-66 years; average, 38 years) underwent surgical treatment consisting of the Gill procedure and posterolateral fusion for isthmic lumbosacral spondylolisthesis. Seventy-three patients (46 men and 27 women) were available for an average of 3.8 years' follow-up (1.0-7.4 years). Thirty-eight underwent one-level fusion, and 35 underwent two-level fusions. Pedicle screw instrumentation was performed in 69 patients. A postoperative questionnaire including the Roland index, clinical charts, and radiographs were reviewed by an independent observer to assess the postoperative course, clinical results, and fusion status. Twenty-five variables were evaluated to determine which affected the fusion and success rates. Primary radiologic fusion and clinical success rates were 78 and 71%, respectively. There was a strong positive correlation between radiologic fusion and clinical success. Overall, single-level fusions showed an 82% fusion rate, and two-level fusions, a 74% rate. For two-level fusions, a significantly higher fusion rate was achieved with a rigid pedicle screw-fixation system than a semirigid system (79 vs. 57%). For smokers, cessation from smoking postoperatively did not increase the fusion rate, and patients who continued to smoke after surgery showed a significantly higher rate of pseudarthrosis. Worker's compensation status did not affect clinical results significantly. Patients who continued to take nonsteroidal antiinflammatory drugs (NSAIDs) >3 months postoperatively showed significantly lower fusion and success rates (44 and 37%). Single-level lumbar fusion for isthmic spondylolisthesis was equally effective with either rigid or semirigid pedicle screw instrumentation. For multilevel spine fusion in isthmic spondylolisthesis, rigid pedicle screw-fixation systems resulted in a high fusion rate. A smoking history or NSAIDs use postoperatively had strong negative influences on the fusion and clinical success rates.

Adult↗

Natural course of experimental scoliosis in pinealectomized chickens.

STUDY DESIGN: Pinealectomy induces experimental scoliosis in chickens. This study analyzed the natural course and characteristics of the scoliosis that developed after pinealectomy. OBJECTIVES: To investigate the natural course of experimental scoliosis after pinealectomy in chickens and determine its similarity to idiopathic scoliosis in humans. SUMMARY OF BACKGROUND DATA. Pinealectomy affects the systemic hormonal balance and consistently induces progressive scoliosis in chickens. METHODS: Thirty five chickens were divided into two groups, a pinealectomy group (n = 25) and a control group (n = 10). At the age of 3 days, all chickens in the pinealectomy group underwent surgery. Spinal radiographs of all chickens were taken at 2-week intervals until the age of 16 weeks. RESULTS: There was no alteration of spinal alignment in the control group. Seventeen chickens in the pinealectomy group had scoliosis (17 degrees-85 degrees) that featured a three-dimensional spinal deformity consisting of both lateral curvature and vertebral rotation with rib humps. The scoliosis progressed to become slight, moderate, or severe as the chickens grew older. Pinealectomized chickens showed several other differences from chickens of the normal control group, such as poor weight gain, underdeveloped cockscombs, and late onset of egg laying. CONCLUSIONS: The scoliosis developing in chickens after pinealectomy was similar to human idiopathic scoliosis, and thus seems to be a useful model of idiopathic scoliosis.

Age Factors↗

Alkaline phosphatase expression in cultured endothelial cells of aorta and brain microvessels: induction by interleukin-6-type cytokines and suppression by transforming growth factor betas.

Alkaline phosphatase (ALP) activity is markedly high in endothelial cells of the blood-brain barrier (BBB) type but absent from or low in those of the non-BBB type. Interleukin 6 (IL-6) has been identified as a glial cell line-derived factor that induces high ALP activity in cultured aortic endothelial cells. In the present study, we examined the effect of IL-6-type cytokines and transforming growth factor betas (TGF-betas) on ALP expression in cultures of calf pulmonary aortic endothelial (CPAE) cells and porcine brain microvascular endothelial (PBME) cells. Leukemia inhibitory factor, ciliary neurotrophic factor, and oncostatin M, which are known as IL-6-type cytokines, induced high ALP expression in the CPAE cells but not in the PBME cells. ALP levels in these cells were markedly suppressed by culture with TGF-betas. However, in cultured PBME cells, IL-6 and a derivative of cyclic adenosine monophosphate significantly increased ALP activity. Our findings raise the posibility that local concentrations of IL-6, IL-6-type cytokines, and TGF-betas affect the ALP levels in the endothelial cells of aorta and brain microvessels under normal development and also under inflammatory conditions.

Alkaline Phosphatase↗

Angle-splitting ostectomy for reducing the width of the lower face.

A patient with a large, squarish, or broad face who desires a small, round, or slender face can undergo mandibular contouring surgery to reduce the width of the lower face. The successful correction of a prominent mandibular angle by conventional angle ostectomy has been reported. However, in the majority of patients with a widened facial appearance, both the mandibular angle and part of the mandibular body anterior to it are protuberant laterally, so both must be resected. At our clinic, operations to reduce the width of the lower face have been performed on 76 patients (9 males and 67 females) during the last 5 years. Initially, we employed a combination of shaving of the lateral cortex and multistaged curved cuts of the mandibular angle. This operative technique, however, required a high level of skill and was time-consuming. A retrospective study of the results revealed the following additional drawbacks. The objective of this procedure is, in a sense, to create morphologically analogous mandibles in all patients, but on the one hand, the resulting relief of the mandibular angle tended to lack individuality, and on the other hand, the primary purpose of reducing the width of the lower face was not fully accomplished in some patients. With angle-splitting ostectomy, which we performed at a later date, however, the width of the lower face was efficiently reduced, producing a natural relief of the mandibular angle. If the surgeon in charge has precise anatomic knowledge and performs this on the basis of an accurate preoperative diagnosis and an operative plan, he or she will encounter no particular difficulties during the operation. There were no complications, such as hematoma, infection, or asymmetry, and aesthetic benefits were achieved even in patients with facial asymmetry.

Adolescent↗

Sweet's syndrome in acute myelogenous leukemia showing dermal infiltration of leukemic cells.

We encountered a 76-year-old woman with acute myelogenous leukemia (AML) who developed Sweet's syndrome. A biopsy specimen taken from her skin lesion on the upper arm showed an infiltration of numerous neutrophils intermingled with leukemic cells. As far as we know, this is the first report of Sweet's syndrome showing a phenotypically identified leukemic cell infiltration of AML determined as M2 in the French-American-British classification.

Aged↗

Correction of experimental scoliosis by rib resection in the transverse plane.

Experimental scoliosis with the potential for marked progression was treated by rib resection on the concave side of the curve, and the alterations of the rib cage and vertebrae in the transverse plane were investigated. Twenty-four chickens were divided into four equal groups (groups R, P, PR, and C) and pinealectomy was performed at 3 days of age in groups P and PR. In group R, three unilateral ribs were resected at the age of 4 weeks. In group PR, three ribs on the concave side of scoliosis were resected at 4 weeks of age if scoliosis of > 20 degrees developed before the age of 4 weeks. Group C served as a control. Spinal radiographs and computed tomography scans at the apical vertebrae were taken at 20 weeks of age, and spinal deformities were evaluated. Scoliosis developed markedly in groups R and P, whereas it was mild in group PR. The apical vertebrae rotated to the convex side of the curve in all groups, in the same way as it would in human idiopathic scoliosis. In group PR, the Cobb angle and the rotation angle of the apical vertebra were symmetrically suppressed. This study indicated that rib resection might control the progression of scoliosis not only in the frontal plane but also in the axial plane when it was done on the concave side of the scoliosis. Although this experiment succeeded in chickens, application in humans is uncertain.

Animals↗

[Small increase of CR1 and CR3 by C5a-receptors on polymorphonuclear leukocytes in systemic lupus erythematosus].

One of the anaphylatoxins, C5a, is known to increase the expression of the complement receptors, CR1 and CR3, on PMNs which play important roles in the phagocytosis. We measured the expression of these receptors before and after the stimulation with C5a and C5a-receptors (C5aR) on PMNs in patients with systemic lupus erythematosus (SLE). PMNs from 16 patients and 11 normal controls were tested. All the patients with SLE were administered with prednisolone orally and were in the inactive stage. The CR1 expression in SLE was significantly weak (p < 0.01) before and after stimulation with 4.55 nM (50 micrograms/ml) of C5a. There was no significant difference of CR3 expression before stimulation. However, after the stimulation with C5a, the increase of CR3 on PMNs from SLE was significantly small (p < 0.01). C5aR on PMNs showed no difference between the two groups. However, the expression of C5aR was significantly suppressed in patients treated with a high dosage of prednisolone (> = 10 mg/day) compared to those with a low dosage of prednisolone (< 10 mg/day). There was no significant difference of CR1 and CR3 expression between these groups. It is concluded that the increase of CR1 and CR3 on PMNs by C5a in small in SLE, of which impaired increase is not due to C5aR on PMNs, and that the expression of C5aR is suppressed by prednisolone.

Antigens, CD↗

Contact dermatitis of the ear due to a rubber earplug.

We report a case of contact dermatitis of the ear canal due to rubber earplugs. A 31-year-old Japanese woman had a 2-year history of eczema confined to her ears. Applications of various topical steroids had shown no beneficial effect upon her lesions. Considering contact dermatitis as a possible diagnosis we performed patch tests. Positive eczematous patch test results were obtained with mercaptobenzothiazole, tetramethylthiuram disulfide and her rubber earplugs, which she had used whenever she took a bath. The eruption cleared after the patient stopped using the rubber earplugs.

Adult↗

[Demonstration and characterization of CD 46, membrane cofactor protein, in gastric cancer tissue].

CD 46, membrane cofactor protein, is a membrane protein which shows different expression and phenotypes with the organ or cell in the same individual. Previously, we reported that gastric CD 46 was expressed strongly in the mucosal epithelium, mucosa and endothelial cells of vessels in the submucosal layer. Western blot analysis revealed that gastric CD 46 was expressed as one broad band with a molecular weight ranging from 60 kDa to 69 kDa, which was different from that of lymphocytes. In this study, we investigated the differences in expression and characterization of CD 46 in gastric cancer obtained by surgery as compared with non-cancerous mucosa. Expression of CD 46 was greater in gastric cancer than in non-cancerous mucosa. In some cases, the phenotypes of CD 46 in gastric cancer were different from those in the non-cancerous tissue.

Aged↗