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Biomedical subjects

M Doenhoff

Publications and source records attributed to M Doenhoff.

27 records · Page 2Linked to original sources
PubMed1978

Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. 2. The time at which resistance to reinfection develops.

Mice developed a partial resistance against homologous challenge with Schistosoma mansoni as early as two weeks after primary infections of 35 to 75 cercariae, and the degree of protection increased to an apparent maximum by 6 weeks. Animals given a primary infection of only 25 cercariae required a longer period to acquire maximum resistance.

Animals↗
PubMed1978

Studies on the host-parasite relationship in Schistosoma mansoni-infected mice: the immunological dependence of parasite egg excretion.

CBA mice deprived of their T cells by means of thymectomy and anti-thymocyte serum and subsequently infected with Schistosoma mansoni were found to have substantially fewer parasite eggs in their faeces than similarly infected immunologically-intact control animals. The number of parasite eggs deposited in the tissues of T-cell deprived mice was by comparison only marginally lower than in control mice. Administration of serum obtained from normal mice with chronic S. mansoni infections partially restored the egg excretion rate in infected deprived mice, and also resulted in an increased number of eggs being deposited in the liver and intestine of these animals.

Animals↗
PubMed1975

The effect of Rauscher and Moloney leukaemia virus on amyloid development in casein-treated CBA mice.

Infection of adult CBA mice with Rauscher or Moloney leukaemia virus concomitantly with caseination significantly accelerated spleen amyloid development in irradiated, bone-marrow protected mice, but had no effect on untreated, adult thymectomized or thymectomized irradiated mice. Spleen tissue of mice infected with Moloney virus had the highest titre in the mice with accelerated amyloid development.

Amyloidosis↗
PubMed1974

Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens.

Human lymphocytes from spleen and tonsils have been cultured with a variety of polyclonal mitogens. Cultures consisted of either unseparated T and B cells or alternatively purified T or B lymphocytes. The purity of the starting cell populations and the origin of activated lymphoblasts was analyzed with a panel of seven markers which discriminate between T and B cells. The selectivity of the lymphocyte responses was influenced by cell populations in a given culture, the mitogen used, and to a limited extent on culture conditions. Purified T lymphocytes from tonsil and spleen responded to phytohemagglutinin (PHA), pokeweed mitogen (PWM), and staphylococcal enterotoxin B (SEB). Purified B cells from spleen responded well to PWM, weakly to SEB and lipopolysaccharide, but not at all to PHA. Tonsil B cells responded weakly to PWM and SEB but not to PHA. Some B lymphocytes do respond to PHA in the presence of activated T cells. These results are discussed in relation to previously reported selective responses of human cells and parallel studies in animal species.

B-Lymphocytes↗
PubMedDate not supplied

The association of steroids and schistosomicides in the treatment of experimental schistosomiasis.

The best therapeutic approach to acute schistosomiasis (Katayama fever) is still unsettled. In this paper we report a synergistic effect between schistosomicides and steroids in the treatment of the early stages of Schistosoma mansoni infection in the mouse. CBA mice infected with 150 S. mansoni cercariae were treated with oxamniquine or praziquantel and dexamethasone or prednisolone. The rate of parasite egg excretion by treated mice and appropriate controls was monitored, and the mice were perfused 43 d after infection for estimation of worm burdens and tissue egg densities. Mice treated with schistosomicides alone or with schistosomicides plus steroids had worm burdens of similar size. Significant reductions in egg counts were, however, recorded in faeces, and in the intestines and livers (with consequent reduction in liver pathology), of mice treated with schistosomicide and steroid, when compared to mice treated with schistosomicide alone or steroid alone. The apparent inhibition of fecundity of S. mansoni by combining these drugs has clear implications for treatment of the Katayama syndrome.

Animals↗