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Biomedical subjects

M Drăgan

Publications and source records attributed to M Drăgan.

8 recordsLinked to original sources

[Use of Drăgan's high-speed PBT staining method in oral cytology examinations (author's transl)].

Drăgan's high-speed PBT (polychrome blue tannin) staining method enables cellular elements to be detected in oral smears in one minute at most. The basic dyes in the polychrome blue solution allow the acid-group-containing substrate to be detected in the nucleus, in the cytoplasm of epithelial cells and leukocytes, as well as in the elements of the flora. The chromatin shows a distinct basophilic, orthochromatic reaction. The fine, reddish-violet metachromatic granules in the cytoplasm are of a glycolipid nature and occur with the tonofibrils. The keratohyalin granules show a basophilic orthochromatic or metachromatic, homogeneous or granular reaction in relation to the chemically acid portions of their structure.

Carcinoma, Squamous Cell

Bacterial immunofluorescence in the intestinal juice of patients with nonspecific chronic enteropathy.

The bacterial immunofluorescence (BIF) in the intestinal juice was studied by the method of Virginia Thomas in a group of 26 patients with nonspecific chronic enteropathy (NCE). Our investigation revealed BIF in jejunal juice in 16 of 26 patients with NCE. The BIF test may be useful for the estimation of immune response to pathogenic germs at the level of the intestine.

Bacteria

Trichomonas vaginalis--identification by rapid BPT-Drăgan staining.

The blue-polychrome-tannin rapid staining (BPT-Drăgan, 1968) applied on fresh and dried vaginal smears makes possible the identification of different forms of Trichomonas vaginalis (TV) parasite. The nucleus is coloured in blue-violet and the cytoplasm in various degrees of blue-green, violet or bichromatical, with blue and violet zones at the cell periphery. In the cytoplasm, the staining allows the identification of many granules and vacuoles. All the stainable substrates have an acid character and represent morphohistochemical aspects which reflect the parasite metabolism in different functional and evolutive stages. Our results allow the recommendation of the method in the screening actions of the TV identifications.

Animals