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Biomedical subjects

M Dukić

Publications and source records attributed to M Dukić.

At least 19 recordsLinked to original sources
PubMed2006

Oxidative stress in the rats brain capillaries in sepsis--the influence of 7-nitroindazole.

As a part of blood-brain barrier, brain capillaries participate in pathophysiological events during systemic inflammation. We investigated the effects of 7-nitroindazole (7-NI), selective neuronal nitric oxide synthase (NOS) inhibitor, to oxidative status (OS) of brain capillaries. Adult Wistar rats were randomized at groups: control group (CG) (sham operated), sepsis group (GS) (cecal ligation and perforation with inoculation of Escherichia coli (ATCC 25922), 7-NI group (G7-NI), (30 mg/kg b/w i.p.) and 7-NI + sepsis group (G7-NIS), (7-NI was applied 30 minutes before operation). Lipid peroxidation index (LPI), nitrite concentration, superoxide dismutase (SOD) activity and superoxide anion (O2*-) content were determined 3, 6, 24 and 48 hour in each group. Cerebral capillaries were separated from non-vascular brain tissue using sucrose gradient. Compared to controls, LPI, nitrite and O2*- increased at SG. In the G7-NIS, LPI reached control values at the 24th and 48th hour, while nitrite were decreased at the 3rd and 24th hour, compared to controls. In the same group, O2*- decreased at the 3rd, 6th and 24th hour, although SOD showed variable activity. The systematic nNOS inhibition with 7-NI forces OS on early terms of sepsis, but lately it contributes to the normalization of OS in cerebral capillaries.

Animals↗
PubMed1989

[Hereditary and congenital defects in children in the regions of Indija-Stara Pazova and Pancevo-Kovin].

An analysis of frequency and range of hereditary and congenital defects was performed in all infants born in the period from January 1 to December 31, 1986 in the Ward for Neonates at the Department of Gynecology and Obstetrics in Panĉevo and in the out-patient maternity home in Indija and Kovin. In Panĉevo 2.559 (1.286 m. and 1.273 f.) newborn infants were born, i.e. there were 2.314 (1.178 m. and 1.136 f.) mature children and 245 (108 m. and 187 f.) premature children. In Indija 367 (180 m. and 187 f.) newborn infants were born, i.e. there were 355 (174 m. and 181 f.) mature children and 12 (6 m. and 6 f.) premature children. In Kovin 146 (65 m. and 81 f.) newborn infants were born, i.e. 150 (82 m. and 68 f.) mature and 10 (1 m. and 9 f.) premature children. A total 3.072 (1.531 m. and 1.541 f.) newborn infants in all three places were born. In all three places there was a total of 2.805 (1.417 m. and 1.387 f.) mature and 267 (115 m. and 152 f.) premature children. In Panĉevo 68 children were born with defects (40 m. and 28 f.), i.e. 2.66% (3.11% m. and 2.20% f.). In Indija only one defect was noted in one female child (0.53% related to female newborns).(ABSTRACT TRUNCATED AT 250 WORDS)

Congenital Abnormalities↗
PubMed1978

[Assessment of the state of the fetoplacental unit before induction of labor and its outcome].

By a prospective study the authors tried to assess the state of the fetoplacental unit in pathologic pregnancies. Depending on the given situation, the following analytical procedures were used: oxytocin test (OCT), placental beta-1-glycoprotein (SP-1), amnioscopy, and analysis of the liquor amnii ingredients (L/S relationship). By combining the above quoted procedures and taking into account obstetric factors (pelvic measures, cervical maturity), the assessment was made of the state of the fetus and the placenta, of the maturity grade and gestation age, and of the condition of the fetus for delivery by the planned induction of uterine contractions. Statistical analysis (the kappa 2 test) has shown that there is no difference in the results concerning the parameters observed, which speaks for good agreement between them. In the authors' opinion, none of the methods used is an absolutely valid test but their combination and dynamic observation along with a certain clinical experience, could lead to right conclusions about the state of the fetoplacental unit before induced labour.

Adult↗
PubMed1975

[Anamnestic reaction].

From 402 pregnant women immunized with blood-group antigens, the anamnestic serologico-immunological reaction has been confirmed in 11 cases (2,7%). Because of the absolute low number of the cases that have been followed up no suggestions are suggested but only observations made with the invitation to professionals to follow up, in an organized form, this interesting phenomenon in blood group immunology.

Antibody Formation↗
PubMedDate not supplied

Oxidative stress in the thalamus of Wistar rats treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

Experimental parkinsonism was induced in adult Wistar rats by selective nigrostriatal neurotoxine, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in a single dose of 0.09 g/kg, by unilateral intrastriatal application using stereotaxic instrument. Control group included rats treated with 0.9% saline solution in the same manner. Animals were sacrificed by decapitation seven days after the treatment. Total glutathione was measured in the crude mitochondrial fraction of thalamus and striatum. Total glutathione content, as a measure of reduced cell atmosphere, was mutually decreased in the thalamus and striatum of MPTP-treated animals, compared to controls: thalamus ipsi- = 24.8 +/- 3.11, contralateral = 26.81 +/- 5.31; striatum ipsi- = 19.96 +/- 4.13, contralateral = 17.3 +/- 4.09 nmol/mg prot. Mutually depleted glutathione content in the thalamus and contralateral striatum, the structures distant from ipsilateral treated striatum, could indicate on spatial propagation of oxidative stress, not only in the selective vulnerable dopaminergic nigrostriatal neurons, but in the structures included in the motor and cognitive loops of basal ganglia.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMedDate not supplied

Effects of nerve growth factor on antioxidative system in the thalamus of MPTP treated Wistar rats.

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism is one of the most useful models for the study of that disease. It has been suggested that MPTP-induced neurotoxicity may involve the production of reactive oxygen species. MPTP was applied intracerebrally, unilaterally, in the striatum in single dose of 0.09 g/kg b.w. The second group was treated both with MPTP and nerve growth factor (NGF) in dose of 7 ng/ml. NGF was applied immediately after the neurotoxin. Control group was treated with 0.9% saline solution in the same manner. Animals were decapitated 7 days after the treatment. In the group treated with MPTP, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) was decreased in ipsilateral thalamus, compared to control values as well as to the contralateral thalamus. In the same structures superoxide anion production was increased, compared to controls. Following the application of both MPTP and NGF, the activity of SOD and GSH-Px remained on control values, while the superoxide anion content was decreased, compared to controls. These results indicate a temporal and spatial propagation of oxidative stress and spread protective effects of NGF on the thalamus, the structure that is distant, but very tightly connected with striatum, the place of direct neurotoxic damage.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMedDate not supplied

Cytochrome C oxidase activity and total glutathione content in experimental model of intracerebral aluminum overload.

Treatment of Wistar rats with aluminum chloride causes astroglial and neuronal cell damage in the selective brain regions of association cortex and hippocampus, seen in patients with Alzheimer's disease. Adult Wistar rats were treated with unilateral intrahippocampal injection of AlCl3 in one single dose of 3.7 g/kg b.w. Control group of animals was treated with 0.9% saline solution likewise. Animals were sacrificed by decapitation seven days after the treatment. Activity of cytochrome C oxidase (COX) and total glutathione content were measured in the ipsi- and contralateral hippocampus and forebrain cortex. Activity of COX was mutually decreased in the hippocampus (ipsi- 30%, contra- 34%), as well as in the forebrain cortex (ipsi- 44%, contra- 47%), compared to controls. These decrease could indicate a deficiency in reducing equivalents with concomitant altered proton gradient and function of electron transport chain, as well as decreased ATP synthesis. Content of glutathione, a clue antioxidative factor, was decreased for about 50% in all examined structures, primary suggesting an impaired regeneration of reduced glutathione. Such distribution of diminished antioxidative defense could be the consequence of the specific brain distribution of transferrin receptors, which was also a main protein carrier for Al. Furthermore, at the cellular level Al could impede glycolysis with consequent decreased production of reducing equivalents which were necessary for glutathione synthesis/reduction, as well as for proton gradient and functionality of electron-transport chain.

Aluminum Chloride↗
PubMedDate not supplied

[Value of the fetal biophysical profile in intensive monitoring of high risk pregnancy].

The fetal biophysical profile (FBP) is a combination of both acute and chronic condition markers of the fetus in the uterus (fetal movements, fetal breathing movements, amniotic fluid volume, maturity of the placenta, intestine distension, cardiotocographic "non stress test", cardiotocographic "oxytocin stress test" cardiotocographic "physical stress test") presented numerically, is an irreplaceable method for monitoring the condition of the fetus in the uterus. In 271 women with high risk pregnancies at the gestational age of 34-44 weeks, depending on the FBP value, the time and mode of pregnancy termination were chosen. There is a significant difference regarding the outcome of pregnancy (general bad outcome--p less than 0.001, mortality--p less than 0.004, low 5 min Apgar score--p less than 0.002, fetal distress during labour--p less than 0.001) depending on the FBP value (normal or pathological) and the connection of the pathological FBP values with the low fetal biochemical profile values (estriol in 24th urine less than 40,000 n mol/day and HPL less than 5 mg). Sensitivity (46.4%), specificity (99.2%), the prognostic value of the pathological test PVP (86.7%), and prognostic value of the normal test - PVN (94.1%) show a high FBP value as a diagnostic test. It is necessary to stick to the protocol while using this test which is very simple, cheap and not at all harmful.

Apgar Score↗